Parasitology Flashcards

(71 cards)

1
Q

Parasite definition

A

Dependance on another for food and shelter

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2
Q

Endo vs Ecto parasites

A

Endo (inside)
- protozoa
- worms
Ecto (outside)
- insects
- arthropods

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3
Q

Cestodes cause problems for which host?

A

intermediate

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4
Q

What are the 5 types of hosts?

A
  • definitive host (where parasite becomes sexually mature)
  • intermediate host (temp environment for parasite to complete lifecycle)
  • paratenic (transfer host) (remains alive until can infect another host but doesn’t undergo development)
  • vectors (intermediate hosts and carriers)
  • reservoir hosts (harbour infection transmitted to humans)
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5
Q

What are parasite factors?

A

Dose
Virulence (ability to cause damage)
infectivity

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6
Q

What are host factors?

A

Innate resistance (genetic ususally)
previous exposure
passive immune status (neonates)
age
Reproductive status (preg, lactating vs non preg, sterile vs intact)

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7
Q

What are environmental factors?

A

Animal stocking density
animal movemnt between groups (rotational vs continous grazing)
Quarantine of new stock
Houseing (ventilation, sanitation)
Enviro conditions (temp, humidity, rainfall)
Nutrition (protein, energy and macromineral/ micromineral balance)

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8
Q

Host factors affecting parasitism?

A

age
exposure
amount of parasites
genetics
periods of stress
preg/lactating
management factors

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9
Q

Definitive host

A

parasite reaches sexual maturity and undergoes repro in this host

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10
Q

Reservoir host

A

non human definitive host which maintains parasite for possible human infection

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11
Q

Endoparasite

A

inside
protozoa
worms

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12
Q

Ectoparasite

A

outside
insects
arthropods

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13
Q

How is pathology produced?

A

production of something toxic or foreign material excereted
migration through tissues
host reaction (allergies/inflammation)

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14
Q

Host specific parasites

A

Narrow host range
well adapted
niche relation

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15
Q

Non host specific parasites

A

broad host range
possibility of paratenic hosts (resoivoirs)
zoonotic possibility

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16
Q

Arthropod

A

joined limbs
hard exoskeleton
head, thorax and abdomen
90% of all species on planet

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17
Q

Ectoparasite path

A

direct trauma
removal of metabolites and blood
allergic irritaion and dermititis
interference with functioin or behaviour

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18
Q

Ectoparasite vectors

A

Mozzies
Tsetse fly
Bufflo fly
Blackflies
Midges, snadflies
fleas
ticks (arachnid)

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19
Q

Types of vectors

A

Mechanical (infection directly to host –> not essential for lifecycle)
Biological (undergo development or multiply (essentail for life cycle

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20
Q

WHat are the HOT rumunanet nematodes

A

H barbers pole
O small brown stomach worm
T Black scour worm

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21
Q

What are the general features of parasitism (DAD)

A

Diarrhoea
Anaemia
Death

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22
Q

Conditions for encystment

A

loss of food source
* dessication
* increase in tonicity (salts)
* change in O2 concentration
* change in pH
* temperature change
* or terminal stage of a life cycle (ie. oocyst coccidia)

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23
Q

4 major protozoa groups

A

Amoebas
Flagellates
Cilliates
Sporozoa

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24
Q

How is malaria transmitted

A

Sporozoited injected with saliva
enter circulation
trapped in liver (recpetor ligand)

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25
Which malaria is the worst and why?
Falciparum (constant high fevers that barely come down)
26
What is a pellicle
combination of plasma membrane and thin, translucent, secreted envelope IN FLAGELLETE
27
Tritrichomonas foetus causes what in cows
infertility abortion up to 5 motnhs after breeding
28
What is the gold standard?
The “artificial insemination” industry prescribes for bovine trichomonosis a rigorous protocol of six weekly T. foetus negative cultures for bulls older than 365 day of age. GOLD STANDARD
29
Water borne disease
Cryptosporidiosis caused by Cryptosporidium spp. feacal oral diarrheoa NO TREATMENT
30
Food borne disease
Trichinellosis (trichinosis) caused by Trichinella spp. through meat (worms) reproduce in body and babies go into muscles Pigs intermediate host
31
What are examples of secondary tests
Oxidase Catalase O/F (Oxidative/fermentation
32
What are examples of tertiary tests
Acetoin CAMP Carb fermentation Clumping Enteropluri lactose fermentation motility Oxi/ferm sorbitol sucrose urea trehalose
33
How would you best control strangles
Quarantiine
34
What is an obligate parasite?
a parasite that depends on a host for nourishment, repro, habitat and survival. Can't survive away from host.
35
What is a saprophyte
An organsim that gets its nourishment from dead or decaying organisms Fungi
36
Give an example of an obligate parasite
Flea, tick, louse, blood fluke, takeworm etc.
37
Classes of Mycoses
– Opportunistic mycoses – Dermatophytes – Systemic mycoses – Mycotoxicoses
38
Opportunistic mycoses
saprophytes mucosal commensals Chronic lesions (reponse resembles that to foreign bodies)
39
Aspergillus
Opportunistic mycoses Ubiquitous saprophytic moulds Cause respitory disease Outbreaks occur when large numbers of conidia are inhaled or penerate eggs
40
Aspergillus fumigatus
conidial nd hyphal adhesions triggery proinflammatory cytokines (cell wall) exoenzymes catalase siderophores melanin (free radical scavenger) Caseating tubercle-like lesion in which hyphae may be seen Horses - topical tratment (antifungal) Clean environment, sanitise and ventiation (all animals) Cause encephilitis in eggs
41
Cryptococcus neoformans
Yeast – Spherical cells single bud attached by a slender stalk – Surrounded by a polysaccharide capsule – Grow rapidly (2-4 days) on blood agar or Sabouraud agar – Creamy white colonies variably mucoid – Worldwide distribution – Serotypes A – C. neoformans var grubii – Serotypes B & C – C. neoformans var gattii – Serotype D – C. neoformans var neoformans – Ecological niche * Grows well in bird poo – C. neoformans var. gattii = Certain eucalyptus species provide saprophytic niches – Tropism for nervous tissue – Virulence – Capsule * Inhibits phagocytes * Activates the alternative complement pathway * Suppresses leucocyte migration * Depresses antibody synthesis – No evidence of transmission between animals – Environmental exogenous infection – Inhalation * Nasal, sinuses and brain – Direct entry via wounds – Granulomatous lesions – Disease usually occurs in animals with cell mediated immunity deficit (except C. neoformans var. gattii) – Young – AIDS patients – FIV in cats – Koalas ?? * Housing in niches * Koala susceptibility – C. neoformans var. gattii virulence – Diagnosis – Latex cryptococcoal agglutination test (LCAT) * Detects the presence of capsular antigen in serum or CSF * Crypto-LA test
42
Dermatophytes
Fungi associated with the skin - Microsporum * M. canis → carnivores (cats and dogs) * M. gypseum → horses - Trichophyton * T. equinum → horses * T. verrucosum → cattle
43
Classification of Dermatophytes
Geophilic – Inhabit the soil – Infections of animals due to prolonged exposure of contaminated soil – Sporadic – Not easily spread from animal to animal (not very contagious) – Hair in soil enhances infectivity – Infections promoted by wet conditions and biting insects Zoophilic – Specialised skin parasites of animals – Some easily infect species other than preferred host – Young animal in close contact ✓ * Contagious – High humidity and temperature✓ – Skin trauma and poor nutrition ✓ – Concurrent disease ✓ Anthropophilic – Specialised skin parasites of humans – Survive briefly in soil – May infect animal
44
Dermatophyte pathogenesis
– Entry via skin abrasion – Conidia (asexual spores) can survive for long periods in moist environments – Up to 3 years in some species – Hyphae invade walls of hair follicle. – Hyphal tip penetrates the hair cortex both by pressure and by hydrolysing the keratin – Secondary bacterial infection of follicles – Antigens are highly allergenic and induce a delayed hypersensitivity reaction
45
Dermatophytes Diagnosis
– Microscopy examination of skin scrapings – Culture – Sabouraud agar – Dermatophyte Test Medium
46
Dermatophytes: Treatment, prevention and control
– UV light can have some curative effect (sunlight) – Some may be self-limiting – Clipping hair – Topical treatment – Iodine – Imidazoles (inhibit ergosterol synthesis) – Antisepetic rinses and antifungal shampoos – Prolonged treatment necessary – Does not stop spread – Does prevent re-infection – DISINFECT THE ENVIRONMENT !!! – Bleach, Iodine and most common disinfectant – Inanimate objects (fomites), bedding and rugs etc.. – Drying and exposure to sunlight !!! – **Isolation if zoophilic !!
47
Microsporum canis
– Zoophilic – Natural host is the cat – Ringworm in cats and dogs – Can infect many species including humans – Zoonosis – Carriers in cats and dogs – ~ 50% will fluoresce under Wood’s lamp – Colony appearance – Fluffy, white to yellow flat colony with radiating edges – The reverse surface exhibits a lemony to chromic yellow pigment – Microscopic features – Spindle shaped thick walled macroconidia
48
Microsporum gypseum
– Geophilic – Natural soil saprophyte – Infects a wide range of animals and man – Lesions highly inflammatory – Colony appearance – Powdery cinnamon surface – The reverse surface may or may not be pigmented – Microscopic features – Thin walled symmetrical spindle shape macroconidia
49
Trichophyton equinum
– Zoophilic – Horses – Very rarely in humans – Young horses most susceptible – < 4 years old – Most cases occur particularly in humid weather during autumn and winter – Affected animals must be isolated – Contaminated harness and grooming gears disinfected – Bleach (0.5% sodium hypochlorite) – Colony appearance – White, cottony with a yellow edge – Reverse is yellow to red-brown – Microscopic features – Macroconidia rare – Microconidia spherical to pyriform (pear-shape)
50
Trichophyton verrucosum
– Zoophilic – Cattle – Can infect humans – Can survive for many years in farm buildings – May grow on semi-dried faecal material – Calves most susceptible - Lesions around the face and eyes – Most cases occur in the winter – Groups of animal affected – Contagious – Usually, self-limiting – Topical treatment – Colony appearance – Deeply folded, white to brilliant yellow – Grow slowly requires thiamine and inositol – Microscopic features – Macroconidia rare – Microconidia ovoid to pyriform (pear-shape)
51
Mycotoxicoses
– Mycotoxins – Low molecular weight, non-antigenic substance , many are heat-stable – Formed on feeds following growth of fungi under favourable temperature and moisture – Exposure by ingestion (mostly) – Toxins are secondary metabolites – Formed during the stationary phase of culture – Growth may occur on crops or in poorly stored feed – Pigs, dairy and feedlot cattle – Optimum conditions for toxin production vary between species – Varying host susceptibility with age – Disease manifestation – Acute disease with overt signs – Chronic with reduced growth rate or reproductive efficiency – Increase susceptibility to other disease – Diagnosis can be difficult – Absence of specific signs in chronic disease – Uneven distribution of toxin in feed – Instability of some toxins and lack of detection methods – Fusarium graminearum – Aspergillus flavus – Perennial rye grass staggers – Claviceps spp. – Pithomyces chartarum
52
Aspergillus flavus
- produce extremely toxic aflatoxins, the most toxic being aflatoxin B1 – A. flavus is ubiquitous and can grow and produce toxin on most solid foodstuffs – Considerable variation in species susceptibility to toxin – Humans cause of food poisoning – Outbreaks occur particularly in warm moist conditions – The toxins inhibit RNA and protein synthesis and cause hepatic centrilobular necrosis and fatty infiltration – Acute disease may cause sudden death, or there may be less acute forms with haemorrhage, jaundice and anorexia – Chronic disease may cause reduced feed conversion and enhanced susceptibility to concurrent disease. – Intoxication of sows may cause abortion with few other signs – Aflatoxins are highly carcinogenic – Control and prevention – Minimise storage time (time from harvesting to feeding) – Monitor feed – Ammonia gas to detoxify contaminated feed – Dilute infested feed to reduce concentration (no longer acceptable in EU)
53
Aflatoxin detection
– Feed and tissue – Thin-layer chromatograpy * Examined under UV * B1 and B2 is blue – High performance liquid chromatography – Immunoassay – Biological assay * Ducklings * Chicken embryos * Shrimp larvae * Trout embryos
54
Conditions for encystment
* loss of food source * dessication * increase in tonicity (salts) * change in O2 concentration * change in pH * temperature change * or terminal stage of a life cycle (ie. oocyst coccidia)
55
Isospora suis (Cystoisospora suis)
neonatal porcine coccidiosis * pale, watery diarrhoea * decreased growth * 5-15 day old piglets * high morbidity & low mortality
56
WHat does Neospora caninum do to which animal
Abortion in cows * no clinical signs in cows that abort * mostly in the second trimester of pregnancy * autolysis but no gross pathological lesions * seropositive congenitally infected heifers have a 7.4 times increased risk of abortion in their first pregnancy
57
Toxoplasmosis
Brain cyst Epidemiology * domestic and wild cats main transmitters * oocysts in cat faeces most likely source (children) * raw or undercooked meat (adults) * causes abortion in sheep death in kangaroos Multiple births in cattle
58
What are nematodes?
roundworms
59
How do nematodes infect?
Feacal oral injection
60
Trichostrongyloid nematodes (genrea of importance)
HOT *Haemonchus (“Barber’s pole”) *Ostertagia (“small brown stomach worm”) *Trichostrongylus (“black scour worm”)
61
Trichostrongylid nematodes- general features of parasitism
Ill thrift- poor condition, lethargy Scouring- poor condition, fly strike Anaemia- pale mm, bottle jaw, lethargy Basically DAD: Diarrhoea, Anaemia & Death
62
Trichostrongylid nematodes- host factors
* Age (young and lactating most susceptible) * Nutrition (protein enhances immunity) * Stress * PPR (around birth and lactation) * Resistance due to the immune response (IR) * Resilience – the ability to produce under parasitism
63
What are trematodes?
Flukes
64
WHat are cestodes
Tapeworms
65
Where do trematodes live
water
66
Where do cestodes live
prey intermediate host exploite predator prey relationship
67
Where do nematodes live?
grass and soil
68
Other features of Trematodes
1. Associated with water 2. Indirect life cycles – snails as IH; 3. Many have wide host range (F.hepatica) 4. Most persist for years in the host so... 5. Cause chronic disease
69
What causes bottle jaw?
Liver fluke
70
Diagnosis and control of Trematodes (flukes)
* Signs are weakness, pale mucous membranes due to anaemia (blood loss) and often jaundice (yellowing) * Drenching (TCBz) * Non-chemical control (pipe water and keep dams/creeks fenced) * Vaccination (exptn) * Selective breeding (n’yet) * Biological control (of IH?)
71
Cestode life cycle
From rabbit to dog when dog eats rabbit Rabbit gets it from faecal eggs shead on ground