past questions Flashcards
(126 cards)
1
Q
this gland is not a specific pheromone producing gland:
- Rabbit face gland
- Gerbil’s umbilical gland
- Dogs cicumanal gland
- Hamster’s hip gland
A
Rabbits face gland
2
Q
which is not a method for genetic quality control of lab animals:
- Skin transplantation
- SSLP (micro - satelite)
- Biomedical markers
- Back gross breeding
A
Back gross breedig
3
Q
name a drawback of preference tests?
A
animals choose on the basis of short term conditions
4
Q
which measurement is used to express energy requirement of laboratory animals?
A
Metabolic body weight
5
Q
Sentinels are?
A
animals used to evaluate a group’s health status
6
Q
which method would you use to get 1ml of blood from a rat?
A
cardiac puncture
7
Q
you cannot buy this diet from a commercial producer:
- Commercial pellet
- chemically defined diet
- liquid diet
- gel
A
chemically defined diet
8
Q
which is not an alpha 2 agonist:
- xylazine
- detomidine
- Tolmetine
- romofidine
A
Tolmetine
9
Q
which is not an inherited disease of a rat?
- Deafness
- Hypo-acidosis
- diabetes
- obesity
A
Hypo-acidosis
10
Q
the basic method of rodent phenotyping is to look for?
A
chromosoma test
11
Q
which is Not part of refinement of an animal experiment?
- alternatives to animal use
- genetic homogeneity
- health status
- proper defined diet
A
alternatives to animal use
12
Q
which animals are covered by 63/2010 EEC directive?
A
Vertebrates and cephalipodes
13
Q
GLP not concerned about conditions under which laboratory:
- studies are planned
- are built
- reasearch is performed
- studies are recorded and reported
A
are built
14
Q
which species can die after oral penicilin administration?
A
guinea pig
15
Q
what is an important mechanism of nutrigenomics ?
A
changing the signal pathway
16
Q
acute toxicity test has an invasiveness category of _ pain?
A
severe
17
Q
what is the maintenance energy requirement of lab animals?
A
0.45 MJ DE x W0.75
18
Q
this disease is not a zoonosis?
- lymphatic choriomenigitis of mice
- salmonellosis
- listeriosis
- ringtail
A
ring tail
19
Q
what is the aim of recommended nutrient allowences
A
to ensure the reproduction and longevity of the animals
20
Q
which cannot be a stage of mouse genital and urinary syndrome:
- Ulceration
- bacteraemia
- inflammation
- mating overload
A
ulceration
21
Q
Quarantine period is not defined by:
- Health status of the animal
- species
- number of animals
- purpose of use
A
number of animals
22
Q
which species has tight skin, so cannot grab it without causing pain to the animal?
A
Guinea pig
23
Q
which is not a physical euthanasia method?
- T61
- cervical dislocation
- maceration
- decapitation
A
maceration
24
Q
which species is not a member of rodentia genus?
- golden hamster
- marmoset
- gerbil
- guinea pig
A
marmoset
25
OECD do _not_ deal with questions about
* validation of in vitro methods
* GLP
* co-ordination of toxicological databases
* validation of national laws
validation of in vitro methods
26
Gerbil is a good model for
Epilepsy aorta
27
this animal cannot be examined by ultrasound:
* Rabbit
* guinea pig
* golden hamster
* dog
Golden hamster
28
which is _not_ true for chronic mycoplasmosis of mice
* agent is mycoplasma pulmonis
* zoonotic
* 12-18 day old puppies are most susceptible
* can appear with sendai virus
zoonotic
29
what lab animal is closest taxonimically to the syrian hamster?
Gerbil
30
what lab animal is good to use for auditory research?
Chinchilla
31
which is a breeding technique which is _not_ used to maintain an out-bred stock
* brother sister mating
* random breeding
* MAI
* cross-breeding
brother sister mating
32
A contaminated breeding line should be:
* euthanized
* put under negative pressure cabin
* treated with antibiotics
* individually marked
euthanized
33
in reproductive toxicology, _not_ acceptable treatment
* used only for parents before mating
* between implantation and foetogenesis
* between foetogenesis and parturition
* from parent before mating till separation from dam
only used for parents before mating
34
which part of the mouse can be used for intra-dermal injection
ear
35
pasturella pneumotropica does _not_ attack the
* uterus
* prostate
* brain
* lungs
Brain
36
environmental enrichment should _not_ be done
* just for human fun
* for the benifit of the animals
* with the sense of animals
* with some unpredictability
just for human fun
37
how would you restrain a gerbil
hand around the chest
38
who will decide on institute level if an animal experiment was necessary or not
ethical commite (animal welfare body)
39
it is _not_ a zoonosis
* Hantaan virus disease
* sendai virus disease
* salmonellosis
* lymphocytic - chorio meningitis (LCM)
sendai virus disease
40
female rat will reach breeding maturity at ?
1 month
41
the basic method for rodant sexing?
looking for ano-genital distance
42
the recommended nurtient allowences
ensure the optimum functioning of the animals
43
maintenance energy of lab animals and for extrapolation od dosage use?
w0.75
44
the main body of laboratory animal science in the world is?
CLAS
45
what does 'environmental enrichment' mean in the laboratory
to give bedding material or toys to experimental animals if possible
46
feeding of rancid and/or vitamin E deficient feed may result in
dermatitis
47
zebradanio is an?
omnivore
48
it is an alternative use of animals in experiments
database search and meta- analysis
49
sub-cutaneous injury has a severity category of _ pain
minor
50
what are the three Rs described by Russel and Burch
Reduction
Replacement
refinement
51
it is _not_ true about euthanasia
* must be performed by a researcher or other trained person
* should be used if an animal would suffer in an experiment
* should be planned in experimental protocol
* can be used to get large amount of blood out of an animal
can be used to get a large amount of blod out of an animal
52
what does 'Knock out animal' mean
transgenic animal
53
which method would you use to get a drop of blood from mice
Retro-orbital puncture
54
which name indicated out-bred stock
Ir: NMRI
55
you cannot use this method for rat euthanasia?
* Ketamine
* Decapitation
* cervical dislocation with weight limit
* CO2
Ketamine
56
what is the best equipment to maintain a transgenic colony in
base colony in different countries
57
which species has only provoked ovulation
Ferret
58
what does expression 'inbred strain' mean
brother and sister mating through atleast 20 generations
59
which species is the best for atherosclerosis research
WHHL - rabbit
notes - gerbil
60
you can find a 'sacculus rotundus or ampulla ilei' in the
rabbit
61
this gland is a specific pheromone producer gland
hamster's lateral gland
62
which lab species has a gall bladder
golden hamster
63
what does SPF mean
species pathogen free
64
how would you restrain a rat for per-os dosing
by grabbing the back of the neck (scruff)
65
which sentence is _not_ true for environmental enrichment
* device in case that animal can control
* all posibilities should be done to improve animal's well - being what the experiment allows
* always should be evaluated on cross-benifit base
* a nest material is an example of that
device in case that animal can control
66
what are gnotobiotic (=axenic) animals
Germ free
67
what does rederivation mean?
production of germ-free strain by embryo transfer
68
in the practice, it is not possible to produce germ free colony from this species
* rat
* golden hamster
* rabbit
* duck
duck
69
which would be the best feeding regime in an experiement
Ad lib
70
what is well being
state of physiological and psycological harmony of the animal with its internal and external environment
71
which vein can be used for IV injection in a ferret
V. jugularis externa
72
how would you restrain a rabbit
bu grabbing as much skin on the back as possible
73
you _cannot_ use this method for euthanasia on new-born, nest living animals
* cutting of neck
* cervical dislocation
* CO2 inhalation
* electric stunning
CO2 inhalation
74
which method would you use to get 10ml of blood from an adult rat?
preparation of abdominal aorta
75
which mouse strain is sensitive to Pneumocystis carni
Swiss
76
which is _not_ necessary before transport of rodent?
* fasting
* clinical examination
* ear printing
* acclimatisation
fasting
77
when this phenomenon disappears, the narcosis ends with death
corneal reflex
78
it is _not_ true about euthanasia:
* should be cheap
* should achieve rapid unconciousness and death
* should be safe for user
* should be simple for administer
should be cheap
79
which one is not true about the co-isogenic strains:
* the new gene is a product of naturat maturation
* it can only be maintained in a homozygous form
* the name contains the strain/sub - strain and the symbol of the mutant gene
* the co - isogenic strain only differs by one gene from the original
it can only be maintained in a homozygous form
80
a dog is not a good model for?
* tumour - cancer research
* Leptospirosis
* epilepsy
* cardiovascular atherosclerosis
cardiovascular atherosclerosis
81
what is the liveweight of a micro pig
20-45kg
82
experimental design _cannot_ be
* cross over design
* latin square design
* cross breeding
* factorial design
cross breeding
83
the basic circadian rhythm is dependent on?
gene expression of a protein
84
melatonin is produced by the?
pineal gland
85
this species us used mostly in viral respiratory research
ferret
86
a chronic stress can cause this type of behaviour
Apathy/ aggression
87
well-being _cannot_ be measured by
* Clinical assesment
* pysiological parameters
* grimace score
* reproductive state
reproductive state
88
which enviroment is good for animals
classical music
89
which tissue is least sensitive to suffering
brain tissue
90
the naked mole rat has an extra-ordinary long lifespan because of
special hyaluronan which prevent cancer
91
the co-efficient on inbreeding
means the fraction of the genes that get fixed in a homozygous state
92
it is an inherited disease in some mouse strains
deafness
93
which is a member of the pox viridae
Ectomelia virus
94
what causes ringtail
too low relative humidity
95
which is _not_ part of refinement of animal experimentation
* hair colour
* health status
* analgesia
* environmental enrichment
hair colour
96
what is the hearing range of rodents
0.5/1-100/120 kHz
97
which species has one pair of teats
guinea pig
98
which species has a 'peg' (double incisor teeth)
Rabbit
99
the two important words for animals concerning pain feeling ( weiss - trial)
predictability and controlability
100
mouse pox is caused by
Ectomelia
101
this is not a physical euthanasia method
* T61
* cooling in refrigerator
* cervical dislocation
* decapitation
cooling in refrigerator
102
feature of the african clawed frog ( xenopus)
shows mating behaviour after Hcg injection
103
which is _not_ a feature of the axolotl
* carnivorous
* capeable of regenerating lost limbs
* has a long lifespan ( 8-10 years)
* reaches adulthood after a complete metamorphosis
reaches adulthood after a complete metamorphosis
104
laboratory animals - except the guinea pig usually adjust their feed intake to satisfy their?
energy requirement
105
which species can die after oral antibiotic treatment
rabbit and guinea pig
106
what is the role of PPAR
to burn fats
107
which of the following has _no_ anti-cancer effects
* green tea
* resveratrol
* Iron
* CLA
iron
108
what has an important role in the mechanism of epigenomics
Methylation of DNA
109
what is the lifespan of Zebra fish
6-8 years (according to dick wad)
3-5 years according to internet
110
which has _no_ role in epigenomics
* sulfurinization
* change of base in DNA
* DNA - methylation
* Histon acetylation
change of base in DNA
111
the role of _ is a fine regulation of the protein synthesis in the cell:
miRNA/ siRNA
112
in some cases the epigenetic changes may be inherited
if the effect(s) acts during germ-cell proliferation ('time window')
113
you can find neoteny in
Axolotl
114
pheromones are detected by
VNO
115
you can prevent constant estrus by using GnRH agonist implants in which species
Ferrets
116
the cephalopods protection
protected by the 2010/63/EU directive since 2013
117
what is true about animals in an outbred stock
they are anisogenic
118
chromorhydachyrrhea is caused by
vitamin A deficiency
119
it can pass through the uterine wall
mouse mammary tumour
LCM
120
carbohydrate ingestion stimulates
the fatty acid synthase gene expression
121
drug that may increase the mitochodrial number
resveratrol
122
what is the most used model for drug metabolism studys in humans
Macaco
123
describe the naked mole rat as an animal model
* have a gene which concerves collagen
* do not age
* do not get tumours
* lifespan of 20-30 years
* complex social system and hierachy
* best model for aging and cancer studies
124
explain the pain scoring system for glires
describe the evaluation of the glires pain categories
* 0 points = none
* 1 point = mild
* 2 points = moderate
* 3 points = severe
* based on apperance, behavioir, pulse, appetite/ drinking, cardiovascular, digestive and nervous systems
* 0-4 = normal
* 5-9 = needs analgesia
* 10-14 = moderate pain, possibly need to terminate trial
* 15-20 = not physiological, stop the trial
125
explain the epigenetics mechanism of action
* modifications - e.g. methylation, histone acetylation, which protect DNA and modify which genes are expressed or silence
* Does not change genome itself
* may be inherited
* influenced by outside stimuli e.g. nutritional status
126
give the meaning of the pyrmid conception with examples. shortly describe the pyramid conception, with examples
* computer - synthesize compounds
* bacteria - test for teratogenicity
* cell culture - eliminate harmful compounds
* invertebrates
* fish
* mammals
* non-human primates
* humans
