Patient Preparation

Question 1

Abscess superficial

Answer 1

Wipe area with sterile saline or 70% alcohol.

Question 2

Superficial deep

Answer 2

Wipe area with sterile saline or
70% alcohol.

Question 3

Blood or Bone Marrow Aspirate

Answer 3

Disinfect veni-puncture site with 70% alcohol.

Question 4

Amniotic, abdominal, ascites (peritoneal), bile, joint (synovial), pericardial, pleural

Answer 4

Disinfect skin with iodine preparation before aspirating specimen.

Question 5

Bone

Answer 5

Disinfect skin before surgical procedure.

Question 6

Cerebrospinal Fluid (CSF)

Answer 6

Disinfect skin with iodine or
chlorhexidine before aspirating specimen.

Question 7

Inner ear

Answer 7

Clean ear
canal with mild soap solution.

Question 8

Outer ear

Answer 8

Wipe away crust with sterile saline.

Question 9

Conjuctiva

Answer 9

None

Question 10

Aqueous/vitreous fluid

Answer 10

Prepare eye for needle aspiration.

Question 11

Corneal scrapings

Answer 11

Clinician should instill local anesthetic before collection.

Question 12

IUD

Answer 12

Disinfect skin before removal.

Question 13

IV catheters, pins

Answer 13

Disinfect skin with alcohol before removal.

Question 14

Gastric aspirate

Answer 14

Collect in early AM before patient eats or gets out or of bed.

Question 15

Gastric biopsy

Answer 15

None

Question 16

Rectal swab

Answer 16

None

Question 17

Stool (feces)
routine culture

Answer 17

None

Question 18

O&P

Answer 18

Collect three specimens every other day at a minimum for outpatients:
hospitalized patients (inpa-tients) should have a daily specimen collected for 3 days; specimens from inpatients hospitalized more than 3 days should be discouraged.

Question 19

Bartholin cyst

Answer 19

Disinfect skin with iodine preparation before collection.

Question 20

Cervix

Answer 20

Remove mucus before collection of specimen.

Question 21

Endometrium

Answer 21

None

Question 22

Urethra

Answer 22

Collect 1 hour after patient’s last urination.
Remove exudate from urethral opening.

Question 23

Vagina

Answer 23

Remove exudate.

Question 24

Prostate

Answer 24

Clean urethral me-atus with soap and water and massage the prostrate through the rectum.

Question 25

Urethra

Answer 25

None

Question 26

Hair, Nails, or Skin Scrapings (for fungal culture)

Answer 26

Nails or skin: wipe with 70% alcohol

Question 27

BAL, BB, BW

Answer 27

None

Question 28

Sputum, tracheal aspirate (suction)

Answer 28

Have patient brush teeth and then rinse or gargle with water before col-lection.

Question 29

Pharynx

Answer 29

None

Question 30

Nasopharynx

Answer 30

None

Question 31

Tissue

Answer 31

Disinfect skin

Question 32

CVS

Answer 32

None

Question 33

Straight catheter (in and out)

Answer 33

Clean urethral area (soap and water) and rinse (water).

Question 34

Suprapubic aspirate

Answer 34

Disinfect skin

Question 35

Cul-de-sac

Answer 35

None