pbr322

Question 1

Describe the nomenclature of PBR322.

Question 2

What is the significance of ORI ?

Question 3

List out the two most important features of PBR322.

Question 4

How many restriction sites are in PBR322 ?

Question 5

How does PBR322 work as a cloning vector ?

Question 6

List out the selectable markers in PBR322

Question 7

Write the use of PBR322.

Question 8

What is PBR322 plasmid ?

Question 9

Define PSC101.

Question 10

How is Ti plasmid used in Genetic Engineering ?

Question 11

What is the function of Ti Plasmid ?

Question 12

Sketch out the Ti plasmid vector

Question 13

Mention the use of Ti plasmid in Genetic Engineering.

Question 14

Why are bacteriophage used as vectors ?

Question 15

What are bacteriophage vectors ? Name two commonly used phage vectors

Question 16

Why are Plasmids and bacteriophages used as cloning vector ?

Question 17

How can bacteriophage be used to clone a gene ?

Question 18

State the advantages of lambda phage vector.

Question 19

Define M13 Phage vector.

Question 20

Denote the structure and function of bacteriophage.

Question 21

Define lambda phage vector

Question 22

Define cosmid Vector.

Question 23

Differentiate between plasmid and cosmid.

Question 24

Write the advantages of the use of a cosmid vector.

Question 25

Define YAC.

Question 26

Define BAC.

Question 27

Differentiate between YAC and BAC.

Question 28

Write the component's of Yeast artificial chromosome.

Question 29

How cosmids are constructed ?

Question 30

Define PJB8 vector.

Question 31

Define PYAC3 vector.

Question 32

Write the full form of ATCC abd MTCC.

Question 33

What is DNA ?

Question 34

What is RNA ?

Question 35

Define Gene.

Question 36

Define Genome.

Question 37

Define gene cloning.

Question 38

Define rDNA Technology.

Question 39

How is a recombinant DNA produced?

Question 40

Define gene Therapy.

Question 41

Define genomic library.

Question 42

Discuss the principle of genetic engineering.

Question 43

Identify the applications of rDNA technology in agriculture.

Question 44

Which method is used for strain improvement ?

Question 45

How can microbial strain be improved ?

Question 46

Write the characteristics of strain improvement.

Question 47

Which technology has a great application in strain improvement.

Question 48

What are the main characteristics of industrial strains ?

Question 49

How microbes are useful in industrial production ?

Question 50

List out the common industrial product of microbes.

Question 51

Write the difference between mutation and rDNA technology.

Question 52

How can recombinant DNA be used in industry ?

Question 53

What are the useful products of recombinant DNA ?

Question 54

List out the pharmaceutical products of recombinant DNA.

Question 55

How is genetic engineering used in industrial biotechnology ?

Question 56

How recombinant DNA technology is used in medicine ?

Question 57

How is insulin produced by rDNA technology ?

Question 58

What is the function of restriction enzyme in rDNA Technology ?

Question 59

Define vector

Question 60

List the types of vector

Question 61

Identify the role of plasmids in Genetic Engineering.

Question 62

Name commonly used vectors in Genetic Engineering.

Question 63

Differentiate between pathogens and vectors

Question 64

How do cloning vectors work ?

Question 65

List out the three main components of a cloning vector.

Question 66

Why are vectors important in cloning?

Question 67

How is DNA inserted into a cloning vector ?

Question 68

Why plasmid is used as a cloning vector ?

Question 69

List the different types of cloning vectors.

Question 70

Define cloning vector.

Question 71

Define expression vector.

Question 72

Distinguish between cloning vector and expression vector

Question 73

Who discovered plasmid in biotechnology ?

Question 74

Write the importance of plasmid in biotechnology.

Question 75

Why are plasmids essential for rDNA technology?

Question 76

Define Genetic Engineering.

Question 77

Define PSC101.