Pcr Flashcards by Finlay Herschell

Q

What temperature is denaturing

A

95

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Q

What temperature is annealing

A

55 degrees

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Q

What temperature is annealing

A

55 degrees

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Q

What temperature is extension at

A

75 degrees

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Q

Pcr conrolls

A

Positive : DNA template that is know to have sequence of interest

Negative: lack of either DNA template or DNA polymerase

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Q

P

A

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