PCR

Question 1

What is a polymerase chain reaction (PCR)

Answer 1

-In viro amplification of DNA fragments
-Technique used to amplify one sample of DNA thousands of times to create a large enough DNA sample for extensive analysis

Question 2

Uses of PCR

Answer 2

1. parental tests
2. Detecting mutation
3. Forensics

Question 3

Step 1 of PCR

Answer 3

Denaturation
-DNA fragments, primers, DNA polymerase added
-Heat DNA sample to 95
-Hydrogen bonds break, separating the DNA strands

Question 4

Step 2 of PCR

Answer 4

Anneal of DNA
-Temp is cooled to 55, so 2 primers bind to separate DNA strands
-Primer attachment signals DNA polymerase where to start synthesizing new DNA

Question 5

Step 3 of PCR

Answer 5

Extension
-Temp up to 72
-2 DNA polymerase attaches to primers on the 2 strands and moves along the strand
-Creating new complementary DNA as they move along

Question 6

In vivo vs in vitro clonng

Answer 6

in vivo
+Useful to introduce a gene in another organism
+Very accurate-mutations rare
+Produces large quantities of transformed organisms