per Flashcards
1
Q
what are the components of a pcr
A
template dna
dna polymerase
reaction buffer
mg2+
dNTPs
pcr primers
2
Q
what is the ideal template dna
A
high molecular weight
fully purified
between 500-2500 pg
3
Q
dntps
A
deoxynucleotide triphosphates
4
Q
annealing
A
binding to target dna sequence to form a small region of dsDNA
5
Q
pcr optimisation
A
- alter annealing temperature
- alter primer concentration
- alter mgcl2 conc
- dntp concentration
6
Q
what controls are needed
A
environmental control
method control
pcr negative control ( water)
pcr positive control ( dna of known quality and quantity)
7
Q
what are multiplex pcrs
A
target several different single coy targets at the same time
8
Q
what are the benefits of using multiplex PCRs
A
saves time, precious dna , and reagents, which makes it cheaper
