PERIPHERAL BLOOD FILMS

Question 1

PERIPHERAL BLOOD FILMS:
Should be made within _____ after blood collected using EDTA.9/L

Answer 1

3-4 hours

Question 2

Possible problems that can be encountered in certain conditions:
EDTA-induced platelet ____
EDTA-induced platelet ____

Answer 2

satellitosis
clumping

Question 3

Most convenient and most used technique type

Answer 3

Wedge method

Question 4

Requires at least two glass slides

Answer 4

Wedge method

Question 5

Wedge method
dimensions:

Answer 5

75 x 25 mm or 3-inch x 1 inch

Question 6

Wedge method
Size of drop of blood:

Answer 6

2-3 mm

Question 7

Wedge method:
Distance of blood from edge of slide:

Answer 7

1 cm

Question 8

Wedge method:
Angle of pusher slide:

Answer 8

30-45 degrees

Question 9

< 30 degrees angle of smear

Answer 9

Thinner

Question 10

> 30 degrees angle of smear

Answer 10

Thicker

Question 11

Wedge method
Length:

Answer 11

2/3 or 3/4 of slide

Question 12

Wedge method
Drying of smear:

Answer 12

Small electric fan

Question 13

Widely used stains:

Answer 13

Polychromes stains

Question 14

basic dye stains acidic cellular component (e.g.
nucleic acids)

Answer 14

Methylene blue

Question 15

acidic dye and stains basic cellular components (e.g. hemoglobin)

Answer 15

Eosin

Question 16

Fixative:

Answer 16

Methanol

Question 17

aka Romanowsky stain

Answer 17

Wright’s stain

Question 18

Methyl alcoholic solution of:

Answer 18

Eosin
Methylene blue (50-75%)
Azure B

Question 19

Buffer solution (K+ phosphate / Na phosphate, pH ____)

Answer 19

6.4

Question 20

Too alkaline buffer

Answer 20

excessive blue stain

Question 21

Prolonged staining time

Answer 21

excessive blue stain

Question 22

Inadequate washing

Answer 22

excessive blue stain

Question 23

Thick films

Answer 23

excessive blue stain

Question 24

Too acidic buffer

Answer 24

excessive pink stain

Question 25

Insufficient staining time

Answer 25

excessive pink stain

Question 26

Prolong washing

Answer 26

excessive pink stain

Question 27

Mounting coverslips before they are dry

Answer 27

excessive pink stain

Question 28

HPO WBC estimation can be performed (Factor used:____)

Answer 28

2000

Question 29

HPO Ave. of 5 WBCs/HPF x 2000 =

Answer 29

10,000/uL

Question 30

OIO “_______” track pattern is preferred to minimize distribution errors

Answer 30

Battlement

Question 31

Platelet estimation can be performed (Factor used: _____)

Answer 31

20,000

Question 32

T or F Males have less RBC and Hct than females due to hormones (testosterone)

Answer 32

F More