Permanent blood smear Flashcards
What are blood smears used for?
visualization of leukocytes differential cont, phagocytosis.
Which staining is used for cell cytoplasm in Pappenheim?
May-Grünwald
Which staining is used for cell nuclei in pappenheim?
Giemsa-Romanowski
What is the name of the method using permanent bloodsmear to categorize leukocytes based on morphology after Pappenheim staining?
leukocyte differential count
How many percent of body weigt is blood in mammals?
7-10% (55-90 ml)
What is number of erythrocytes in blood and longevity ?
x10^12
120 days
What is number of Leukocytes in blood and longevity ?
x10^9
depends on cell type
What is number of Thrombocytes in blood and longevity ?
x10^9
9-12 days
Which inorganic substances can you find in blood plasma?
water, Na+, Cl-, HCO-, K+, Ca2+, Mg2+, phosphates, sulphates.
Function: maintenance of osmotic pressure and aAB balance. (buffer systems)
Plasma proteins in blood?
albumins, globulins and fibrinogens.
Function: maintenance of plasma volume, transport of various ions and molecules, proteolytic systems, buffer systems, nutrition, suspension stability.
What are proteolytic systems?
Breaks down proteins–> hemocoagulative, kallikrein-kinin, fibrinolytic and complement systems.
Other organic components in blood?
glucose, triacylglycerols, phospholipids, cholesterol, urea, aminoacids, lactate, nitrogenous non-protein substances, FFA, hormones, vitamines, enzymes (drugs)
