Permanent Mounts

Question 1

All fungi are gram positive

Answer 1

Gram Stain

Question 2

Crystal Violet + Ammonium Oxalate = _____

Answer 2

Hucker Modification

Question 3

to demonstrate details of fungal elements
It is useful in staining tissue specimens
Detects fungi in tissues

Answer 3

Periodic Acid Schiff

Question 4

Periodic Acid Schiff fungi color

Answer 4

pink-red color bright
magenta

Question 5

Periodic Acid Schiff background color

Answer 5

green

Question 6

detects fungi in histologic sections; brown to black.

Answer 6

Gomori Methenamine Silver

Question 7

Gomori Methenamine Silver outline color

Answer 7

black

Question 8

Gomori Methenamine Silver internal parts color

Answer 8

pink black

Question 9

Gomori Methenamine Silver background color

Answer 9

light green

Question 10

Gomori Methenamine Silver detects

Answer 10

Candida
Aspergillus

Question 11

Demonstrates melanin

Answer 11

Gomori Methenamine Silver

Question 12

detection of Nocardia and Blastomyces dermatitidis

Answer 12

Acid Fast (Kinyoun’s)

Question 13

agent of systemic mycoses

Answer 13

Blastomyces dermatitidis

Question 14

is a branching bacteria, not fungi

Answer 14

Nocardia

Question 15

for blood parasites

Answer 15

Giemsa

Question 16

for bloodstain; used in hematology

Answer 16

Wright’s

Question 17

Giemsa or Wright’s stain demonstrates

Answer 17

Histoplasma Capsulatum

Question 18

Demonstrates Malassezia furfur
- causative agent of Tinea versicolor, Pityriasis versicolor (an-an)

Answer 18

Acridine Orange

Question 19

Detects hyphal pigmentation – to determine if hyaline or dematiaceous

Answer 19

Fontana Masson
H&E

Question 20

Acridine Orange fungal element color

Answer 20

green fluorescent

Question 21

Acridine Orange epithelial cell color

Answer 21

orange

Question 22

non pigmented/lightly
pigmented

Answer 22

hyaline

Question 23

darkly pigmented

Answer 23

Dematiaceous

Question 24

Demonstrates Cryptococcus neoformans

Answer 24

Mayer’s Mucicarmine

Question 25

Gridley’s Stain Hyphae and yeasts color

Answer 25

dark blue-pink

Question 26

Gridley’s Stain Tissues

Answer 26

deep blue

Question 27

Gridley’s Stain background

Answer 27

yellow

Question 28

To detect fluorescence

Answer 28

Wood’s Lamp (UVL)