Pharmaceutical Biotechnology Flashcards
(149 cards)
1
Q
What is pharmaceutical biotechnology?
A
A field that uses micro- and macro-organisms and genetically engineered cells to create pharmaceuticals
2
Q
What are primarily application of techniques of biotechnology?
A
discovery research
product development
3
Q
List difference applications of biotechnology in pharmacy
A
Antibiotic production
Antibody production
Transgenic animals
Gene therapy and Gene silencing
Vaccines
Personalized medicine
4
Q
What is the pharmacists role in biotechnology?
A
product evaluation and selection
pt education and counselling
provision of drug information
assistance in patient monitoring
drug control and preparation
5
Q
Go through the central dogma
A
DNA - transcription –> RNA — translation–> protein
6
Q
What are housekeeping genes?
A
they are responsible for the routine metabolic functions common to all cells
7
Q
What is recombinant DNA?
A
form of artificial DNA that is created by combining two sequences from different sources
8
Q
What is recombinant DNA technology?
A
tech which allows proteins to be produced via artificial means
engineer gene for more protein productivity
produce desired protein in vitro for therapeutic use
9
Q
What is the most used recombinant cells used to mass produce proteins?
A
E. coli
10
Q
List advantages of engineering prokaryotes
A
cultivation of prokaryotes is easy
gene manipulation is easy as plasmid DNA is easy to isolate and manipulate
11
Q
List the steps in DNA cloning
A
- Isolation of gene of interest
- Isolation of plasmid DNA (Cloning vector)
- Manipulation of DNA sequence
a. Cutting- Restriction enzymes
b. Joining- DNA ligase - Transformation of bacteria
- Selection of “correct” bacteria
- Replication of the cells carrying rDNA molecules to get a genetically identical cells or clone.
12
Q
What are the three essential features for cloning vector?
A
1) Origin of replication
2) Dominant selectable marker
3) Restriction sites
13
Q
What is extrachromosomal self-replicating DNA molecules?
A
plasmids
14
Q
Define restriction endonucleases
A
primarily bacterial enzymes that cut foreign DNA into fragments by recognizing specific nucleotide base pairs.
15
Q
Define DNA ligase
A
have the unique ability to link or “paste” together DNA fragments that have been produced by exposure to restriction endonucleases.
16
Q
List methods of transformation
A
Heat shock
CaCl2 Transformation
Lipofectin® and similar molecules
Electroporation
Microinjection
17
Q
To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?
a) MgCl2
b) CaCl2
c) NaCl
d) HCl
A
B) CaCl2
18
Q
Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4
A
C) 3
19
Q
Which of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction endonucleasec) RNA polymerased) DNA polymerase
A
A) DNA ligase
20
Q
Which of the following enzyme is responsible for making a DNA copy from RNA?a) Reverse transcriptaseb) DNA polymerasec) RNA polId) RNA polII
A
A) reverse transcriptase
21
Q
Can two DNAs cut with different restriction enzymes join together to form a recombinant plasmid?
A
Yes, if they are cut at the same place they will match
22
Q
Compare small molecule drugs and biological drugs
Based on Size
A
small
low molecular weight
large
high molecular weight
23
Q
Compare small molecule drugs and biological drugs
Based on structure
A
simple, well defined, independent of manufacturing process
complex defined by the exact manufacturing process
24
Q
Compare small molecule drugs and biological drugs
Based on modification
A
well defined
many options
25
Compare small molecule drugs and biological drugs
Based on manufacturing
produced by chemical synthesis
predictable chemical process
identical copy can be made
produced in living cell culture
difficult to control from starting material to final API
impossible to ensure identical copy
26
Compare small molecule drugs and biological drugs
Based on characteristics
easy to characterise completely
cannot be characterised completely the molecular composition and heterogeneity
27
Compare small molecule drugs and biological drugs
Based on stability
stable
unstable, sensitive to external conditions
28
Compare small molecule drugs and biological drugs
Based on immunogenicity
mostly non-immunogenic
immunogenic
29
What was the first example of biotech products?
insulin
30
List the phase of growth curve of bacteria
LAG Phase:
Number of bacteria does not change with time in lag phase.
LOG Phase:
Number of bacteria increases exponentially in log phase.
STATIONARY Phase:
There is no net change in number of bacteria with time in stationary phase. Bacteria divide but also die at same rate.
DEATH Phase:
Number of bacteria decreases with time
31
What is Rifamycin B?
a substance produced by bacteria
it both inhibit transcription by binding prokaryotic but not eukaryotic RNA polymerases
32
What does the structural model of rifampicin indicate?
that rifampicin sterically blocks the path of RNA elongation when the transcript becomes two or three nucleotides long
33
Why can't bacteria always be used to make all drugs?
bacteria do not show some post translational modifcation
34
What is different about the expression system in bacteria?
they are not capable of producing glycoproteins because they lack the capacity to glycoylate
35
List some post tranlational modifications
phosporylation
glycosylation
ubiquitination
nitrosylation
methylation
acetylation
lipidation
36
list some areas where cell culture technology is currently playing a major role
model systems for basic cell biology
toxicity testing
cancer research
making drugs
37
What are the 2 classes of cultures of animal cells?
primary cells
cell lines
38
What is primary culture?
when cells are surgically removed from an organism and placed in a culture to grow, divide and live
most have a finite lifespan
39
Explain Hayflick's Phenomenon
cell will continue to grow and divide normally for a limited number of passages
the number of passages decrease when cells are harvested from older individuals
40
What is a cell line?
if the cells in a cell strain undergo a process that makes them "immortal"
41
What are some processes used to make cells immortal?
chemical or gamma
viral infections with antigen
42
Define suspension cell culture
derived from cells which can divide and
survive without being attached to a substrate,
e.g. cells of haemopoietic lineage.
* Can be maintained in culture vessels that are
not tissue-culture treated.
* Requires agitation for adequate gas exchange.
* Easier to passage
43
Explain Adherent cell culture
* Must adhere to a surface to survive
* Form monolayers e.g. cells derived from
different tissues (breast, liver)
* Growth is limited by surface area
* Will cease proliferating once they become
confluent (completely cover the surface of cell
culture vessel)
* Cells are dissociated enzymatically or
mechanically from surface
44
What is the purpose of culture vessels?
provide a contamination barrier to protect the cultures from the external environment while maintaining the proper internal environment
45
What does the cells vessels do for anchorage-dependent cells?
provide a suitable and consistent surface for cell attachment
46
List the types of culture vessels
cell culture dishes
multiwell plates
flaska
47
What is the main reasons for surface coatings?
it is to give something for the cell line to attach to
make the palate more hydrophilic
48
What are some effects of biological contamination?
They compete for nutrients with host cells
* Secret acidic or alkaline by-products that cease the
growth of the host cells
* They also produce H2O2 which is directly toxic to
cells
49
What is one way that is used to prevent contaminates by different microorganisms?
Why is this not always recommended?
adding different antibiotics
it may help develop resistant microorganisms
50
What are the two most common cell types used for biotherepeutic proteins?
CHO (hamster)
murine (mouse)
51
Why are CHO cells used?
PTMs are similar to humans (glycostylation)
reduced susceptibility to certain viral infections
grow in suspension cultures
serum free chemically defined media
allows gene amplification
high productivity
highly tolerant to changes in pH, Oxygen levels, pressure and temperature
52
How to get protein out?
plants --> seeds
cow --> Milk
Chicken --> eggs
53
Define transgenesis
process of introducing foreign or exogenous DNA into an animal's genome
54
Why transgenesis?
Improve genetic features of domesticated Animals
*Provide animal models for study of human diseases
*Pharming using farm animals for production of human
pharmaceuticals
*Study gene regulation and development of animals
55
Transgenic animals as bioreactors
Whole animals can serve as bioreactors to produce proteins
* Gene for a desired protein (Drug) is introduced via transgenics to the target cell
* By using cloning techniques, cell is raised to become an adult animal
* Produce milk or eggs that are rich in the desired prot
56
Explain retrovirus mediated transgenesis
infecting mouse embryos with retroviruses before the embryos are implanted
size of transgene is limited
57
Explain pronuclear microinjection
introduces the transgene DNA at the earliest possible stage of development of the zygote
DNA is injected directly into nucleus of egg or sperm
58
Why express rProtein in Milk?
Easy to purify - few other proteins in milk
* Doesn’t harm transgenic animal- no change in physiology
* rProtein is authentically modified post-translationally
* Large quantities
* Renewable source
59
What is Atryn?
it is an anti blood clotting protein derived from goats on this farm (antithrombin)
it is produced from the mammary glands of transgenic goats and harvested from their milk
it is the first drug made from genetically engineered animals approved by FDA
60
What are some concerns with transference of diseases?
There is also an added concern with cows & chickens, because the fear of
transference of diseases through drugs, such as mad cow disease and bird
flu.
* Leakage of transgene.
* Ethical concerns on using animals as machines for making drugs.
61
What is EPO?
it is used to treat anemia
it has been genetic engineered to be produced from mammals like sheep or cows
this is a replacement of erythropoietin
62
Define biotech pharmaceutical
is simplu any medically useful drug whose manufacture involves microorganisms or substances that living organisms produce
63
List the types of biologics
hormones
blood products
monoclonal antibodies
cytokines
growth factors
vaccines
gene and cellular therapies
targeted therapy
fusion proteins
64
What are the two-tiered cell banking?
master cell bank
working cell bank
65
What needs to be verified for MCB?
that you have the cell line you wanted to produce
it is not contaminated (bacteria & viruses)
66
Explain the produce of commercial production of recombinant human erythropoietin product
recombinant host cells from master cell bank is mixed with specially formulated media
these cells are grown to very high numbers in media
growth continued until there are enough cells to inoculated thousands of roller bottles
cells grow in roller botles and secrete r-HuEPO into media
then it is containg media is harvested
prufied
solution
packaging
67
What are some sources of variation in biologics?
cloning
protein expression
production
68
Define bioreactors
any device or system that supports a biologically active environment
69
Explain cloning of specific gene into DNA vector
gene seq should be same
70
Explain recombinant DNA plasmid
vector diff
possibility of variation -should be same vector
--> company may not disclose which vector they use
71
Explain cell expansion
same - diff cell line
diff expression
72
What is the goal of an effective bioreactor?
to control, contain and positively influence the biological rxn
(cells are growing and dividing)
73
What are the steps of process design of bioreactor?
upstream steps
production
downstream
74
In general cells can be cultivated either in vessels containing an appropriate liquid growth medium in which the cells can be either:
free in suspension
attached to microspheres or entrapped in matrices
immobilized state as monolayers
75
What are the types of bioreactors?
liquid (submerged) -->more common
solid state (surface)
76
What are the types of bioreactors products?
stirred tank - most preferred
airlift
microcarrier
77
List the bioreaction parameters (influence growth)
* Controlled temperature
* Sufficient substrate (usually a carbon source)
* Sugars, proteins and lipids
* Water availability
* Salts (for nutrition)
* Vitamins
* Oxygen (for aerobic processes)
* Optimum pH
* Product addition and by-product removal
78
List some of the parts of the stirred tank
motor
controller
nutrient feed
oxygen probe
sparger
baffle
temperature probe
79
What is the purpose of the motor?
movement
impeller mix nutrients - distributes
80
What is the purpose of the controller?
control pH
81
What is the purpose of baffle?
stops from spilling --> disturbs vortex made from stirring --> maintain homogeneity
82
What is the sparger?
air bubbles
make sure homogeneity
83
Define airlift bioreactor
* A bioreactor in which the reaction medium
is kept mixed and gassed by introduction
of air or another gas (mixture) at the base
of a column-like reactor.
84
List some characteristics of micro-carrier bioreactors
can provide extremely high productivity within a compact size
has been used widely for culture of immobilized mammalian cells
use porous glass beads to provide a large surface area for cells
85
1. Spargers are used in fermenters for
A) Monitoring temperature
B) Introducing air-bubbles of optimal size
C) Increasing frothing
D) Increasing pH
E) Increasing vortexing
answer is B
86
2. Which is the most widely used bioreactor
A) Fixed bed bioreactor
B) Fluidised bed bioreactor
C) Stir tank bioreactor
D) Air-lift bioreactor
E) Surface bioreactors
C
87
3. The most important function of Baffles in a bioreactor
A) Prevent vortexing and ensure homogeneity of the culture media
B) Prevent infection by bacteria and viruses
C) Ensure that the products produced are not of high viscosity
D) Optimal pH of the culture media is maintained
E) Optimal temperature of the culture media is maintained
A
88
What is the modes of bioreactors operations?
batch operation
continuous operation
fed-batch operation
89
What are some advantages and disadvantages of batch ?
Easy to operate and control
Genetic stability of organism could be controlled if it is genetically engineered biocatalyst.
Lower contamination risk
Non-productive downtime is a disadvantage
Batch to batch variability is problem
Accumulation of inhibitory products is problem
90
What are some advantages and disadvantages of continuous?
Degeneration of biocatalyst
Higher contamination risk is a disadvantage
Efficient, higher productivity
Product is obtained with uniform characteristics; quality of the product is almost same from time to time
No accumulation of inhibitory
products
91
What is fed batch cultivation?
a modification of batch cultivation in which the nutrient is added intermittently to a batch culture
92
List how many times you add media, product harvest, culture/cells for batch
once
once
once - constant
93
List how many times you add media, product harvest, culture/cells for fed batch
>1
once
once - constant
94
List how many times you add media, product harvest, culture/cells for continuous
continuous > 1
continuous > 1
once - constant
95
What is downstream processing
any treatment of culture broth after fermentation to concentrate and purify products
96
Why is product recovery important?
economical
usually the product is available in very dilute form
removal of contaminants
scaling up should be considered
97
What is needed for e.coli and yeast in order to product and recover?
grown in large fermenters at high cell density
cell lysis required
protein is harvested and purified
rapid isolation of protein necessary
98
What is needed for mammalian cells in order to product and recover?
grown in large scale cell culture roller bottles
protein secreted into media
isolation of protein from medium
product in media
99
What are inclusion bodies?
protein aggregates
overproduce proteins through the use of plasmid expression
100
What are some advantages of protein inclusion bodies?
can easily be recovered to yield protein with >50% purity
aggregated forms of protein are more resistant to proteolysis
101
What are some disadvantages of protein inclusion bodies?
inaccurate assessment of recovery
recovery requires cell breakage, protein sedimentation and pellet washing
dissolution and refolding
102
What is intracellular product?
breaking of cells and removal of cell debris using centrifugation - generation of crude protein solution
103
What is extracellular product?
recovery of cell culture medium by removal of cells using centrifugation or filtration
104
What are some factors that are determined by glycosylation?
(1) Solubility
(2) Stability
(3) Serum half life
(4) Pharmacological function
(5) Immunogenicity
105
What is quality of bioprocess design?
measured of product purity and product consistency
106
In order to achieve optimal growth of cells it is important that conditions such as:
pH
O2
temperature
proper nutrients
107
What are some media used for mammalian cell cultures?
fetal calf serum
growth factors
hormones
sugars
amino acids
electrolytes
vitamins
108
What are the potential contaminants?
host related
product related
process related
109
What is the most frequent source of virus introducted?
animal serum
110
List some methods for reducing viral contaminants
heat treatment
radiation
dehydration
neutralization
chromatography
filtration
precipitation
111
What are pyrogens?
are potentially hazardous substances (normally from gram negative bacteria)
112
What are some general sources of protein contaminants?
(a) growth medium used
(b) the host proteins of the cells and
(c) ligands from affinity columns used in the purification process.
113
What is the 3 steps of biopharm -eutical production?
1. upstream (sterilize, get chemicals + media ready)
2. Bioreactor (produce drug)
3. Product recovery
114
What does purification processes yield?
should yield potent protein with well-defined characteristics for human use from which all contaminants have been removed
115
What process can be used to further purify the proteins?
conventional chromatographic separations
116
How does chromatography work?
basic prodcedure is to flow the protein mixture solution through a column packed with various materials
117
List the phases of chromatography
stationary phase
mobile phase
118
Define the stationary phase
insoluble matrix with which components of the mixture interact with during separation
119
Define the mobile phase
solution that is passed through or over the stationary phase, carrying with it the components of the mixture
120
If your protein is positive which ion exchange do you use?
cation exchange chromatography
121
What is charge on resin in cation exchange?
negative
122
What are the steps in quality control of biologic drugs?
plasmids and host cells
protein stability
process validation
final product batch
123
Explain characterization of plasmids and host cells
Stability of the inserted gene must be monitored for host cells
cell growth is monitored (know when to extract bacteria)
124
Explain protein stability
it is determining the protein content
AA sequencing, peptide mapping and high performance liquid chromatography (HPLC)
also use western blot analysis/ELISA test
125
What does 1a/b bind to?
antigen
126
what does 2 a/b bind to?
1 a/b
127
What has fluorescent tag in indirect delection?
2 a/b
128
What has fluorescent tag in direct detection?
1 a/b
129
What does immunofluorescence detect?
detection molecule gives off fluorescence once attached to the specific protein
this is also know as western blot analysis
130
What does ELISA detect?
detection molecule is an enzyme that converts substrate to product
131
What are similarities between ELISA and western blot?
techniques used in diagnostics
both methods are based on immuno detection
they are based on the formation of the antibody-protein complex
can analyze proteins
132
What are differences between ELISA and western blot?
western is time consuming
also takes well trained and skilled personnel to carry out western blotting
133
Explain process validation
it is a revalidation of purification process
spiking the pre-purification mixture with endotoxings and ensuring they are removed during the purification process
134
Explain the final product batch package
many of the tests are done and then repeated
ensure the purified protein has maintained its activity
tests for concentraion and potency are performed
135
What are some stability testing done?
freezing
heating
denaturing
136
What is amino terminal heterogeneity?
it is verifying that the protein have a authentic NH2 terminus to make sure it will be treated right in the body
137
Why can most proteins not be sterilized by standard methods?
it would denature at high temperatures
138
When formulating a biotech a number of issues need to be addressed including ...
route of administration
delivery system used
ability for target specific delivery
stability of the protein
139
List some types of excipients used
1. Solubility Enhancers
2. Anti-adsorption and anti-aggregation agents
3. Buffer components
4. Anti-oxidants
5. Preservatives
6. Active Ingredient
140
Approaches that can be used to enhance solubility include:
proper pH and ionic strength conditions
addition of AA
Addition of surfactants
adding sugars as well
141
What are anti-adsorption agents?
added to reduce adsorption of the active protein to interfaces
142
What is surface phenomenon?
a protein adsorbed to a surface will return to the aqueous solution
143
What can cause aggregates?
exposed AA results in the formation of aggregates as they interact with hydrophobic residues on other proteins
144
What is the purpose of insulin adsorption?
to prevent adsorption of insulin to the surfaces other molecules
this is often albumin which has a high affinity for these surfaces
145
List some common buffer agents
phosphate
citrate
acetate
146
Why is buffer selection important part of formulation process?
because of the pH dependence of protein solubility and physical and chemical stability
147
List some AA that are readily oxidized
methionine
cysteine
tryptophan
tyrosine
histidine
148
List some antioxidants
vitamins A, C and E
AA - acetylcyteine
chemicals - Acetic acid, sodium citrate and selenium
149
Define perservatives
natural or synthetic chemicals that are added to prevent decomposition by microbial growth or by undesirable chemical changes
prevent bacterial growth
