Pharmaceutics Final Flashcards
(77 cards)
Accuracy
degree of conformity with a standard
Precision
degree of perfection within an instrument or method
Repeatability
Ability to get the same results under the same conditions, in the same, lab, on the same day
Reproducibility
Ability to get the same results under similar conditions on a different day, in a different laboratory
Analytical blank
All of the reagents involved in an assay except the analyte
Calibration
comparison of the value for a particular parameter to pre-set standards
detection limit
lowest amount of analyte that can be detected but not accurately quantified
quantitation limit
lowest amount of analyte that can be detected and quantitated accurately
linearity
ability to obtain results within a range that are directly proportional to conc of analyte
range
interval between upper and lower concentrations that the device can accurately measure
robustness
capacity to remain unaffected by small deliberate variations in experimental parameters
selectivity
ability to detect analyte alone in the presence of other compounds
sensitivity
ability to detect small changes in concentration of analyte
one point calibration
utilizes one standard, that is sufficiently reliable, as a comparison value to the analyte where interpolation can be made
multiple point calibration
three points other than zero that generate a comparison range where interpolation and extrapolation (if linear correlation is satisfactory), can ocurr
UV/Vis spectrophotometry
relationship between analyte and intensity of light absorption at a given wavelength
UV/Vis strength + weakness
Strength:
-easy, inexpensive, robust
weakness:
-poor selectivity, hard to analyze mixtures
beer lambert law
A = ebc A: absorbance e: molar absorption coefficient b: length of cell c: concentration
spectrophotometric analysis can…
determine partition coefficient, solubility, and drug release/diffusion
isoproterenol Injection USP standards
may contain not less than 90% and not more than 115% of labelled amount
isoproterenol lab
- isoproterenol
- ferrous sulfate
- aminoacetate
isoproterenol –> analyte
ferrous sulfate –>
aminoacetate –> buffer
phenytoin injection additives
10% alcohol
40% propylene glycol
phenytoin in NS and D5W
precipitated in all concentrations since aqueous solubility is so low
cloxacillin x gentamicin
precipitate
