Photosynthesis Flashcards

(41 cards)

1
Q

What is photosynthesis?

A

A process that synthesises large organic molecules into simple inorganic molecules using the energy from sunlight

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2
Q

What is the symbol equation for photosynthesis?

A

6CO2 + 6H2O ———————> C6H12O6 + 6O2
Light energy

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3
Q

How is stomatal density measured?

A

1) apply clear nail polish to the lower epidermis of a leaf between the veils and allow to dry

2) using forceps, peel the nail polish from the leaf to produce a replica of the lower epidermis

3) place the replica on a microscope slide and count the number of stomata using the microscope

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4
Q

What are the adaptations of a leaf for photosynthesis?

A

— large surface area

— very thin so light penetrates to photosynthetic tissue

— chloroplasts in the palisade mesophyll move in the cytoplasm towards the side with most light

— densely packed with palisade mesophyll cells

— palisade mesophyll cells are densely packed with chloroplasts

— air spaces between spongy mesophyll to allow carbon dioxide to diffuse to photosynthetic cells to maintain the CO2 concentration gradient

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5
Q

Why are cyclic metabolic pathways important to happen inside compartments in the cells?

A
  • reactions can occur in small volumes
  • isolation of enzymes and reactants
  • membranes isolate reactions within organelles, keeping products separate from the cytoplasm
  • mitochondria can be positioned close to areas needing ATP, and organelles can move within the cytoplasm
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6
Q

What/where is the light dependent stage of photosynthesis?

A

LOCATED: in the thylakoid membrane and thylakoid space of the chloroplast

FUNCTION: involves the conversion of light energy into chemical energy (ATP & NADPH)

PRODUCTS: ATP, NADPH, and O2

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7
Q

What/where is the light independent stage of photosynthesis?

A

LOCATION: in the stroma of the chloroplast

FUNCTION: uses the products of the LDR (ATP & NADPH) to produce organic molecules such a glucose

USES UP: ATP and NADPH

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8
Q

What do photosynthetic pigments do?

A

Capture light energy from the sun and convert it into various chemical energy

  • located within the thylakoid membrane of chloroplasts
  • different wavelengths of light strike the leaf and are absorbed by the various pigments
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9
Q

What are the types of photosynthetic pigments?

A

PRIMARY PIGMENTS = chlorophyll a (absorbs blue and red wavelengths of light)

ACCESSORY PIGMENTS = chlorophyll b (absorbs blue and red wavelengths of light)
= carotenoids (xanthophyll and beta-carotene) (absorbs violet/blue light)

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10
Q

Why are accessory pigments important?

A

They absorb wavelengths of light that aren’t absorbed by the primary pigments (chlorophyll a)

This ensures a wide range of wavelengths are absorbed therefore increasing the efficiency of photosynthesis

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11
Q

What macronutrient is needed to produce chlorophyll a?

How is the structure of chlorophyll a aiding photosynthesis?

A

Magnesium

  • one electron is energised from each Mg2+ to a higher energy level when light is absorbed
  • the fatty acid tail holds the chlorophyll molecule in the thylakoid membrane
  • hydrocarbon chain is hydrophobic and fits in the fatty acid tail of the thylakoid membrane
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12
Q

What is chromatography used for?

A

To separate the photosynthetic pigments

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13
Q

How is chomotagraphy performed?

A

1) tear up leaves and grind with acetone to form a dark green pigment solution
2) use a capillary tube to spot the pigments onto the pencil line drawn onto the chromatography paper
3) place the chromatography paper into a solvent (e.g. acetone or petroleum)
4) remove the chromatography paper once the solvent has travelled to the top of the strip and observe the different distances the pigments have travelled
5) calculate the Rf value for each pigments
6) identify each pigment by comparing the Rf values to known values for each pigment

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14
Q

Why do we use acetone as the dissolving solvent?

A

Acetone will dissolve all of the phospholipid membranes, which helps to extract the pigments from the thylakoid membranes in the chloroplasts

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15
Q

How should the pigment be spotted onto the chromatography paper?

A

One drop should be spotted onto the pencil line , allowed to dry, and another spot should be placed on top. Repeat this process until the pigment is dark enough

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16
Q

How should the pigment be spotted onto the chromatography paper?

A

One drop should be spotted onto the pencil line , allowed to dry, and another spot should be placed on top. Repeat this process until the pigment is dark enough

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17
Q

Why is the running solvent necessary?

A

The running solvent dissolves the pigments (solutes) and carries the pigment up the chromatography paper

18
Q

Why do the different pigments travel different distances?

A

The more soluble the pigment is in that specific solvent, the further it will move up the chromatography paper

19
Q

How is Rf value calculated?

A

Distance travelled by the pigment
—————————————————
Distance travelled by the solvent

20
Q

Why should a pencil line be drawn on the chromatography paper and not pen?

A

Ink from a pen has different coloured pigments which will also dissolve, these can be confused with the photosynthetic pigments. This wont happen with pencil as pencil will not dissolve in the running solvents

21
Q

Why is a ratio compared with the solvent front required to be taken?

A

To standardise the method

22
Q

Why does the pencil origin line need to be above the running solvent?

A

If the solvent is above the origin line, the pigments will dissolve straight into the running solvent and will no get moved up the chromatography paper.

23
Q

What is the absorption spectrum?

A

A graph that shows the amount of light absorbed by chlorophyll a and the accessory pigments at each wavelength of light

24
Q

Why is so little green and yellow light absorbed?

A

Green and yellow light is reflected by the green chlorophyll pigments

25
What is the action spectrum?
A graph that shows the rate of photosynthesis at different wavelengths if light
26
What is the relationship between the absorption spectrum and the action spectrum?
There is a close connection between greater light absorption and a faster rate of photosynthesis , suggesting that these pigments are responsible for absorbing the wavelengths of light used in photosynthesis to ,are glucose
27
What is an experiment that can be conduced to measure the rate of photosynthesis?
Count the number of bubbles of O2 gas produced by pondweed (underwater plant) over a set period of time
28
What is the structure of photosystems?
- accessory pigments are grouped into clusters with associated proteins to form an antenna complex - the accessory pigmets absorb photons of light energy and funnel this down the antenna complex to the reaction centre - the reaction centre contains 2 chlorphyll a molecules and is found below the antenna complex - when photons of light energy reach chlorophyll a, electrons are excited to become high energy
29
What is the role of photosystems?
- absorb photons of light energy - act as transducers - transferring the light energy to high energy electrons - this energy is then used to fuel proton pumps and synthesis ATP
30
What is the difference between photosystem 1 (PSI) and photosystem 2 (PSII)
PSI has chlorphyll a that is 680nm (P680) PSII has chlorophyll a that is 700nm (P700)
31
What are the two types of LDR?
- non-cyclic phosphorylation - cyclic phosphorylation
32
Describe how plants absorb light energy from the sun and use this energy to produce useful substances in the light dependent reaction of photosynthesis?
- light energy is passed from the accessory pigments (chlorophyll b, xanthophyll, beta-carotene) to 2x chlorophyll a (P680) in the reaction centre of PSII - photons of lights are also absorbed directly by chlorophyll a -exciting 2 electrons to a high energy state - high energy electrons are released from chlorophyll a, binding to he primary electron acceptor and passing down a chain of electron carriers down the electron transport chain - energy lost from electrons in the electron transport chain activates a proton pump so H= ions diffuse from the stroma into the thylakoid lumen - electrochemical gradient is established, allowing ATP synthesis from ADP and Pi - final electron acceptor is NADP+ which binds to H+, forming NADPH - photolysis of water (2H20 —> 4H+ + 4e- + 1/2O2) - low energy electrons from water replaces those lost from chlorophylll a in P680 and protons to reduce NADP+
33
What is cyclic photophosphorylation?
A process that occurs in bacteria and primitive plants Higher lands use this process to provide extra ATP when CO2 is in short supply, or if levels of NADPH are very high ONLY PSI IS INVOLVED
34
What is the process of cyclic photophosphorylation?
- light energy is absorbed at PSI and channelled to chlorophylll a - electrons in the chlorophyll a become excited, energised, and released. They are then accepted by an electron acceptor and are passed down the same electron transport chain that is used by the electrons from PSII - protons are pumped from the stroma into the thylakoid space and ATP is produced from ADP+Pi - the electrons excited from PS! Return to PS! THERE IS NO PHOTOLYSIS AND NO INVOLVEMENT OF PSII
35
What are the products of cyclic or non cyclic photophosphorylation?
CYCLIC: ATP only NON-CYCLIC: ATP, NADPH, O2
36
What is the Calvin cycle/LIR?
- CO2 from the atmosphere is fixed with RuBP in a reaction catalysed by RuBisCo enzyme - this forms the unstable 6C compound, which splits into glycerate-3-phosphate (3C) - G3P is then reduced, simultaneously oxidising NADPH into NADP+, ATP is also hydrolysed and thee phosphate is transferred to G3P, forming 1 triose phosphate for every G3P (2 per RuBP) - one carbon atoms of the 6 is removed from the Calvin cycle and is used to produce organic molecules like glucose - 5 carbon atoms remain in the Calvin cycle and are used to regenerate RuBP so the cycle can begin again in another process that requires ATP
37
What happens to the amounts of RuBP and G3P when CO2 is low?
When CO2 falls, less RuBP is sued to fix CO2, but it continues to be regenerated from any triose phosphate present, using the ATP in the Calvin cycle so concentration of RuBP increases As less RuBP is used to fix CO2, less of the unstable ^C compound is formed, so less G3P is generated. Any G3P present is still reduced to TP using ATP hydrolysis and NADPH, so G3P concentration falls/decreases
38
What happens to the concentrations of G3P, glucose, and RuBP in the dark?
- G3P increases in the dark, as CO2 is fixed not RuBP, but in the dark there is less light dependent products, so less G3P is reduced to triose phosphate, so [G3P] increases - glucose falls as it is used up in respiration and isn’t replaced by photosynthesis in the dark - RuBP is used to fix CO2 in the dark still, as the reaction is not dependent on products from the light dependent reaction. Concentration falls as it is not regenerated from TP due to a lack of ATP
39
What organic molecules can be produced as a result of photosynthesis?
- amylose (starch) - amino acids - fatty acids - cellulose
40
What is the role of nitrate in plant metabolism?
Taken up by roots, transported as nitrates in the xylem and as amino acids in the phloem FUNCTION: protein synthesis, synthesis of nucleic acids/DNA/RNA/nucleotides DEFICIENCY SYMPTOMS: reduced grout of organs, chlorosis (yellowing leaves due to inadequate chlorophyll production so no longer able to absorb light energy)
41
What is the role of magnesium in plant metabolism?
Absorbed as Mg2+ and transported in the xylem FUNCTION: for chlorophyll production and activation of ATP synthase DEFICIENCY SYMPTOMS: pronounced chlorosis between veins of older leaves as Mg is moved and transported to new leaves