Photosynthesis Flashcards
(41 cards)
What is photosynthesis?
A process that synthesises large organic molecules into simple inorganic molecules using the energy from sunlight
What is the symbol equation for photosynthesis?
6CO2 + 6H2O ———————> C6H12O6 + 6O2
Light energy
How is stomatal density measured?
1) apply clear nail polish to the lower epidermis of a leaf between the veils and allow to dry
2) using forceps, peel the nail polish from the leaf to produce a replica of the lower epidermis
3) place the replica on a microscope slide and count the number of stomata using the microscope
What are the adaptations of a leaf for photosynthesis?
— large surface area
— very thin so light penetrates to photosynthetic tissue
— chloroplasts in the palisade mesophyll move in the cytoplasm towards the side with most light
— densely packed with palisade mesophyll cells
— palisade mesophyll cells are densely packed with chloroplasts
— air spaces between spongy mesophyll to allow carbon dioxide to diffuse to photosynthetic cells to maintain the CO2 concentration gradient
Why are cyclic metabolic pathways important to happen inside compartments in the cells?
- reactions can occur in small volumes
- isolation of enzymes and reactants
- membranes isolate reactions within organelles, keeping products separate from the cytoplasm
- mitochondria can be positioned close to areas needing ATP, and organelles can move within the cytoplasm
What/where is the light dependent stage of photosynthesis?
LOCATED: in the thylakoid membrane and thylakoid space of the chloroplast
FUNCTION: involves the conversion of light energy into chemical energy (ATP & NADPH)
PRODUCTS: ATP, NADPH, and O2
What/where is the light independent stage of photosynthesis?
LOCATION: in the stroma of the chloroplast
FUNCTION: uses the products of the LDR (ATP & NADPH) to produce organic molecules such a glucose
USES UP: ATP and NADPH
What do photosynthetic pigments do?
Capture light energy from the sun and convert it into various chemical energy
- located within the thylakoid membrane of chloroplasts
- different wavelengths of light strike the leaf and are absorbed by the various pigments
What are the types of photosynthetic pigments?
PRIMARY PIGMENTS = chlorophyll a (absorbs blue and red wavelengths of light)
ACCESSORY PIGMENTS = chlorophyll b (absorbs blue and red wavelengths of light)
= carotenoids (xanthophyll and beta-carotene) (absorbs violet/blue light)
Why are accessory pigments important?
They absorb wavelengths of light that aren’t absorbed by the primary pigments (chlorophyll a)
This ensures a wide range of wavelengths are absorbed therefore increasing the efficiency of photosynthesis
What macronutrient is needed to produce chlorophyll a?
How is the structure of chlorophyll a aiding photosynthesis?
Magnesium
- one electron is energised from each Mg2+ to a higher energy level when light is absorbed
- the fatty acid tail holds the chlorophyll molecule in the thylakoid membrane
- hydrocarbon chain is hydrophobic and fits in the fatty acid tail of the thylakoid membrane
What is chromatography used for?
To separate the photosynthetic pigments
How is chomotagraphy performed?
1) tear up leaves and grind with acetone to form a dark green pigment solution
2) use a capillary tube to spot the pigments onto the pencil line drawn onto the chromatography paper
3) place the chromatography paper into a solvent (e.g. acetone or petroleum)
4) remove the chromatography paper once the solvent has travelled to the top of the strip and observe the different distances the pigments have travelled
5) calculate the Rf value for each pigments
6) identify each pigment by comparing the Rf values to known values for each pigment
Why do we use acetone as the dissolving solvent?
Acetone will dissolve all of the phospholipid membranes, which helps to extract the pigments from the thylakoid membranes in the chloroplasts
How should the pigment be spotted onto the chromatography paper?
One drop should be spotted onto the pencil line , allowed to dry, and another spot should be placed on top. Repeat this process until the pigment is dark enough
How should the pigment be spotted onto the chromatography paper?
One drop should be spotted onto the pencil line , allowed to dry, and another spot should be placed on top. Repeat this process until the pigment is dark enough
Why is the running solvent necessary?
The running solvent dissolves the pigments (solutes) and carries the pigment up the chromatography paper
Why do the different pigments travel different distances?
The more soluble the pigment is in that specific solvent, the further it will move up the chromatography paper
How is Rf value calculated?
Distance travelled by the pigment
—————————————————
Distance travelled by the solvent
Why should a pencil line be drawn on the chromatography paper and not pen?
Ink from a pen has different coloured pigments which will also dissolve, these can be confused with the photosynthetic pigments. This wont happen with pencil as pencil will not dissolve in the running solvents
Why is a ratio compared with the solvent front required to be taken?
To standardise the method
Why does the pencil origin line need to be above the running solvent?
If the solvent is above the origin line, the pigments will dissolve straight into the running solvent and will no get moved up the chromatography paper.
What is the absorption spectrum?
A graph that shows the amount of light absorbed by chlorophyll a and the accessory pigments at each wavelength of light
Why is so little green and yellow light absorbed?
Green and yellow light is reflected by the green chlorophyll pigments