Plant Sciences Flashcards

1
Q

What have plants done for us?

A
  • food
  • buildings (wood)
  • materials
  • clothes
  • paper
  • landscapes
    AND MANY MORE
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2
Q

Global challenges

A
  • food security and nutrition
  • water security
  • energy security
  • medicine and pharmacology
  • environmental sustainability
  • wealth distribution
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3
Q

Norman Borlaug

A

Norman Borlaug won the Nobel Peace Prize 1970 for developing a semi-dwarf, high yield variety of wheat Beforehand, the wheat plants were too tall and so very fragile and subject to damage. He developed a shorter, stronger variety and so is referred to as the father of “Green revolution”

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4
Q

Factors challenging food security

A

Land and Soils
- 25% of the planet’s land is highly degraded
Climate change
- temperatures are exceeding survival thresholds of crop, tree and fish species
Energy
- modern food systems are heavily dependent on fossil fuels
- 85% of total primary energy is fossil fuel bases

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5
Q

World population and food security

A
  • World population will be ~9billion by 2050

- food production will need to double

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6
Q

GM crops

A

genetically modified

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7
Q

Potential benefits of GM crops

A
  • overcome linkage drag, “clean” gene movement
  • introduce novel abilities
  • more rapid breeding cycles
  • increased food production
  • improved human nutrition
  • wealth distribution
  • open up marginal land
  • increase land and water use efficiency
  • reduced environmental impact (CO2 and NO)
  • reduced fertiliser use
  • reduced herbicide, pesticide, fungicide, bactericide use
  • reduced soil damage
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8
Q

Potential problems of GM crops

A
  • commercial interests
  • loss of ecological diversity
  • gene transfer to wild relatives
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9
Q

Are GM crops unnatural?

A

You can also get natural genetically modified crops due to horizontal gene transfer e.g. sweet potatoes are genetically modified by agrobacterium species and are the 7th most important crop.

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10
Q

GM crop summary

A
  • increases yield (22%)
  • decreases pesticide quantity (-40%)
  • decreases pesticide cost (39%)
  • increases total production cost (3%)
  • increases farmer profit (68%)
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11
Q

Solanum Tuberosum (GM crop)

A
  • 3rd most important crop worldwide
  • uses 2/3 water of rice for same calorific yield
  • 25% of global crop lost to disease each year (enough to feed UK for 15 years!)
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12
Q

Phytopthora Infestans (GM crop)

A
  • GM potato
  • causative agent of the irish potato famine
  • new strain “blue13” is able to overcome all current blight resistances

Challenges:

  • more resistant varieties of potato
  • new, less harmful control measures

In the wild, disease is the exception and resistance is the norm, so why do our potatoes get infected?

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13
Q

GM potato - traditional breeding vs GM

A

Traditional: ~25 year per gene moved
GM: ~24 months, independent of gene numbers

  • transfer multiple resistance genes simultaneously
  • multiple resistant potato
  • yield and character maintained
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14
Q

Nitrogen-fixing cereals

A
  • N2 fertiliser use accounts for 5% of global energy (and is increasing)
  • N2 fertiliser allows for ~3-4x increased crop yield
  • expensive
  • 2/3 of applied N2 is lost to environment
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15
Q

Crops for the future

A
  • snorkel rice (flood resistant)
  • sub rice (submergence resistant)
  • C4 rice
  • golden rice - increased vitamin A content
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16
Q

Folk medicine

A
  • morphine, codeine - analgaesic
  • digitalin - heart arrythmia
  • quinine, artemisin - malaria
  • colchicine - gout
  • tansy - embalming, roundworm, threadworm
  • salicylic acid - warts and other skin conditions
  • laudanum - pain killer and cough represent
  • eating daffodils - vomiting, whooping cough, cold and asthma
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17
Q

Drug discovery

A
  • 55% of drugs owe their origins to plants
  • 25% of all drug are still made directly from plants
  • 60% of anti-cancer drugs are of plant origin
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18
Q

MDR malaria

A
  • artemisinin
  • artemesia
  • green small leaved plant
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19
Q

lung/breast/ovarian cancer

A
  • taxol
  • yew
  • pine-like leaves with berries
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20
Q

leukaemia

A
  • vincristine
  • madagascan periwinkle
  • pink/purple flowering plant with small rounded leaves
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21
Q

asthma

A
  • ephedrine
  • ephedra
  • no leaves some small buds
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22
Q

pain, fever, inflammation

A
  • asparin

- willow

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23
Q

chronic and acute pain

A
  • morphine

- opium poppy

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24
Q

alzheimers

A
  • galantamine

- snowdrop

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25
Q

bradychardia

A
  • atropine
  • deadly nightshade
  • quite large pointed leaves with purple flowers
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26
Q

heart arrhythmia

A
  • digoxin
  • foxglove
  • purple/pink cone like flowers
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27
Q

Metabolic diversity of plants

A
  • high
  • naturally produce anti-microbial and anti-herbivory compounds
  • ~20% of all global flora re used in folk remedies
  • <1% of known plant species have been assessed for bioactive compounds
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28
Q

Why start with natural products? (drug discovery)

A
  • pre-screended in folk medicine (ethnopharmacology)
  • active in the cell
  • higher chance of biological activity
  • combinatorial effecs
  • community benefit
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29
Q

Vaccines

A
  • 4-5 weeks after outbreak
  • HIV, viral and bacterial diarrhoea, anthrax, rabies, diphtheria, malaria, alzheimer’s etc.
  • can be edible
  • store as seed for when needed
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30
Q

Plants as medical bioreactors

A
  • many post-translational modifications are maintained, can also be “humanised”
  • maintain stereochemistry
  • cheap to grow
  • store as seed for when needed
  • edibility
  • interleukin, interferon, factor VIII, hGH
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31
Q

Energy security

A
  • e.g. waste miscanthus is used as straw on farms
  • algae + CO2 = oil
  • can use “dirty” water and waste CO2
  • capture waste NPK from agricultural run off
  • remove heavy metal pollutants
  • algal residue contains many useful compounds
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32
Q

Plants as chemical bioreactors

A
  • stereochemistry can be maintained
  • complex syntheses can be achieved through gene stacking
  • perform complex organic chemistry at room temperature in water
  • cheap to grow
  • store as seed for when needed
  • main chassis for synthetic biology
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33
Q

Are plants conscious?

A
  • perceive light from UV to infra-red
  • detect and differentiate touch
  • smell/taste more than any animal
  • conduct electrical impulses and have glutamate receptors
  • long and short term memory
  • they are more complex at the genetic, biochemical and environmental level than any animal
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34
Q

What is the world’s most important food crop?

A
  • maize (corn)
  • USA is biggest producer
  • staple food for the majority of the sub-saharan africa
  • being used more and more for ethanol
  • dometicated about 10,000 mya in southern mexico
  • over 800 million metric tonnes produced
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35
Q

What is the second most important food crop?

A
  • rice
  • over 700 million metric tonnes produced
  • main producer is China
  • may be even more important than corn as a food crop as corn is used for other purposes besides consumption
  • thirstiest crop (need at least 2,000 litres of water per kg)
  • domesticated 11,000-12,000 mya in China
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36
Q

what is polished rice deficient in?

A

provitamin A

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37
Q

Which have been the focus crops of the Green revolution?

A

rice and wheat

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38
Q

Why is there pressure on food security?

A

the yield increases have lagged behind human population growth in areas where rice is a dominant crop for human consumption

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39
Q

Rice genome sequencing

A
  • completed in Jan 2001

- could lead to breakthroughs for higher yields

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40
Q

Rice GM crops

A
  • golden rice

- a cultivar that has been genetically modified to produce higher levels of vitamin A

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41
Q

What is the third most important crop?

A
  • wheat
  • ~700 million metric tonnes produced
  • major producer is China
  • covers more of earth than any other crop
  • resilient, grows in dry and cold climates where rice and maize cannot
  • leading source of vegetable protein for humans
  • domesticated ~10,000 mya in the fertile crescent
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42
Q

What percentage of the world’s food energy intake do rice, maize and wheat provide?

A

60%

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43
Q

How many edible plants are there

A

Over 50,000

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44
Q

What is the fourth most important food crop?

A
  • potatoes
  • over 300 million metric tonnes produced
  • China is major producer
  • not a cereal like the others, the number one non-grain food product
  • grows best in temperate climates
  • originally grown in the Andes
  • Spanish introduced to Europe in 16th century
  • really high in nutrients
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45
Q

How much land is used for agriculture?

A
  • half
  • has a huge impact on the ecosystem and world function
  • ~1/4th of that is crops
  • vast majority = cereals (main three)
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46
Q

Where do we get the vast majority of our calories?

A

cereals

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47
Q

Double fertilisation

A
  • sperm meets egg in ovule (fertilisation)
  • another sperm fertilises the polar nuclei
  • the endosperm is huge in the cereal
  • the endosperm is a food source for the embryo
48
Q

Why were cereal plants domesticated

A
  • they taste good
  • initially has lots of nutrients
  • can be stored
  • basis for civilisation
  • allows us to get on with education, building, developing etc.
49
Q

Crop domestication

A
  • wild cereal
  • cultivation/domestication
  • selective breeding (landraces)
  • modern cereal
50
Q

Domestication of cereals for increase in grain yield

A
  • first step was loss of shattering
  • loss of vernalisation requirement
  • increased seed number
  • reduced seed shattering
  • reduced height
  • reduced dormancy
51
Q

Tough rachis mutation

A
  • loss of seed dispersal (shattering)
  • results in grain remaining attached to the mature ear
  • often considered the most important domestication trait as it makes propagation of the plant dependent on human intervention
  • higher yields
  • can delay harvesting until grains have matured
52
Q

What does the loss of grain dispersal aid?

A
  • e.g. hairs, hooks and awns
  • these facilitate wind and animal dispersive processes
  • natural selection for grain dispersal aids is lost once the ear becomes nonadhescent and partly from human selection for grain morphologies that simplify postharvest crop cleaning
53
Q

Increase in grain size

A
  • can arise by direct selection or via tillage
  • tillage: larger grain surviving deeper burial
  • grain size is often used as an indication of human intervention in plant reproduction
54
Q

loss of sensitivity to environmental cues for germination and flowering

A
  • the grains of most crops germinate too soon after planting
  • the wild versions often germinate only in response to environmental cues such as day length and temperature
  • this is thought to be selected by cultivators using grain from the previous harvest to sow the succeeding crop, as grain that germinates slowly will make a decreasing contribution the the harvesting crop
55
Q

Synchronous tillering and ripening

A
  • selected by cultivation practices especially as these develop into a continuous annual cycle
56
Q

Compact growth habit

A
  • selected by harvesting methods that preferentially sample plants of similar size and shape
57
Q

Enhanced culinary chemistry

A
  • e.g. improved breadmaking quality of wheat and changes to the sugar-starch balance in maize
58
Q

Examples of altered development of non-cereals

A
  • kale (leaves)
  • broccoli (flower buds and stem)
  • cabbage (terminal leaf bud)
  • cauliflower (flower buds)
  • brussel sprouts (lateral leaf buds)
  • kohlrabi (stem)
59
Q

Rice gene qSH1

A
  • controls abscission zone formation at base of rice flower
  • single nucleotide polymorphism (SNP) in 5’ regulatory region of a single transcription factor was responsible for nonshattering rice
60
Q

Maize TGA and TB1

A
  • both encode transcription factors
  • TGA promotes development of tough case around kernal
  • TB1 suppresses branching to promote single stalk
61
Q

The population bomb

A
  • Since 1900, the population has increased from ~1.6 billion to ~8 billion
  • how do we feed all these people?
62
Q

The green revolution

A
  • one of the most significant accomplishments of 20th century science was the development of lodging-resistant, high-yielding semi-dwarf grain varieties
63
Q

Green revolution varieties of wheat had …

A

Gibberellin (GA) hormone biosynthesis

64
Q

GA signalling pathway

A

WAITING ON DIAGRAM

65
Q

Submergence tolerance problem

A
  • more than 16 million ha of land used to grow rice in lowland ares and deep-water areas are unfavourably affected by flooding
  • the estimated annual economic loss of this is more than US$ 600 million
  • climate change will increase flash flooding where most of the world’s rice is grown
  • for deepwater rice, water stands for long periods. If the plant does not elongate sufficiently, it will drown, set poor grain and/or die
66
Q

Submergence tolerance solution

A
  • for deepwater floods, rice needs to escape the water and float
  • SK = snorkel proteins
  • most lowland rice will show stem elongation when completely submerged to try and escape out, the rice uses up its carbohydrates reserves and may die
  • some tolerant landraces show quiescence, enter a dormant, quiescent state rather than try and grow out of the water (conserve energy and survive)
67
Q

What is quiescence controlled by?

A

the SUB1A gene that encodes an ethylene responsive transcription factor (ERF)

68
Q

Submergence tolerece signalling pathway

A
  • submergence
  • ethylene (SK1/SK2 also induced by ethylene but act differently from SUB1A and promote GA-induced stem elongation)
  • ABA/ SLR1 +SLRL1 (dellas)
  • GA responses
  • CHO consumption
  • elongation growth
69
Q

Why couldn’t we live without plants?

A
  • produce most of the oxygen we breathe
  • produce most of the chemically stored energy we consume as food and burn for fuel
  • produce an amazing assortment of useful chemicals
70
Q

Globally, how many people per year are chronically hungry?

A
  • more than one billion
  • more than the total population of the USA, Canada and the EU
  • although a lot of people have a lot to eat they are not very nutritious
71
Q

How many people per year are chronically anemic due to iron deficiency?

A
  • more than 2 billion

- about the total population of the USA, Canada, the EU and China

72
Q

Plant scientists can contribute to the allevation of hunger by developing plants that…

A
  • are drought or stress tolerant
  • require less fertiliser or water
  • are resistant to pathogens
  • are more nutritious
73
Q

Plant-microbe associations (positives)

A
  • essential for C and N recycling
  • important for plant growth (water and minerals)
  • important to strengthen plant health
74
Q

Plant - microbe associations (negatives)

A
  • detrimental to plant health
  • threat to crop production for human food and energy
  • threat to natural ecosystems
75
Q

Plant mutualism

A
  • enhances reproduction and nutrient uptake
  • plants cooperating with other organisms
  • e.g. pollination, seed dispersal, nitrogen fixing endosymbiosis, mycorrhizal symbiosis
76
Q

Mutualistic associations with root symbionts

A
  • with soil organisms

- the plant gains the nutrients, the symbionts gain the sugars derived from photosynthesis

77
Q

Mycorrhizal fungi as a major symbiont

A
  • most plants
  • extensive fungal surface area facilitates nutrient and water uptake
  • grow inside the roots and increase surface area for uptake, root system expanded
78
Q

Nitrogen-fixing bacteria as symbionts

A
  • some plants
  • bacteroid containing nodules form to facilitate nitrogen fixation
  • bulbs on roots
79
Q

Ectomycorrhizal fungi

A
  • proliferate on the outside of the root and between cells
  • not very common
  • don’t penetrate so don’t cause damage
  • found in forests in association with trees
80
Q

Arbuscular mycorrhizal fungi

A
  • enter the plant cell and form tree-like structures
  • the fungus gets sugars produced by photosynthesis
  • the plant gets nitrogen and phosphorus from the soil
  • the arbuscule provides a large surface area for nutrient exchange
81
Q

Root nodule symbiosis

A
  • bacteria form root nodules
  • bacteria live in them
  • ensure plants get nitrogen
  • mainly within plants of the legume family
82
Q

Nitrogen abundant but unavailable

A
  • in the atmosphere, nitrogen exists as dinitrogen gas N2, an unusually inert molecule with a triple bond holding the two atoms together
83
Q

Biological nitrogen fixation

A
  • uses ATP

- N2 + 16 ATP + nitrogenase -> 2 NH3

84
Q

Two-way signalling between rhizobia and plant

A
  • the plant root produces a flavonoid chemical that attracts rhizobia
  • the bacterium produces a Nod factor, identifying it as a symbiont (and not a pathogen)
  • the plant prepares to form a sybiotic nodule structure
  • makes sure plant does not activate defences
85
Q

Do all bacteria produce nod factors?

A

no

86
Q

Decomposition

A
  • dead plant and animal material
  • saprophytes (bacteria and fungi)
  • recycles back into system
  • C and N cycle
87
Q

Saprophytes

A
  • feed off dead/organic matter (plant and animal)
  • fungi and bacteria
  • digest then absorb
  • extracellular (secreted) enzymes
  • carbohydratases, lipases, proteases
  • essential for C and N recycling
  • enzymes usually unique to bacteria and fungi
88
Q

Lignin

A
  • a constituent of the cell walls of almost all dry land plant cell walls
  • 2nd most abundant natural polymer
  • only polymer in plant cell walls that is not composed of carbohydrate monomers
  • only large-scale biomass source of an aromatic functionality
  • composed of up to three different phenyl propane monomers
89
Q

Cellulose

A
  • most abundant natural polymer
  • has carbon (not easy to extract)
  • in the fibres
  • works with lignin to provide a structural function
90
Q

Strategies of pathogenicity

A
  • find a host
  • gain entry through the plant’s impermeable defences
  • avoid plant’s defence responses
  • grow and reproduce
  • spread to other plants
91
Q

Interaction -> Disease

A
  • the pathogen must be able to overcome plant defences
  • the host plant must be susceptible to the pathogen
  • the environment must tip the balance in favour of the pathogen
  • most interactions do not lead to disease
92
Q

Facultative pathogens

A

can attack living plant cells but can also grow by themselves
e.g. on aritficial medium

93
Q

Obligate pathogens

A

can only grow on their specific living host

94
Q

Biotrophic pathogens

A

feed on living plant tissue, not causing cell death
e.g. hyaloperonospora arabidopsidis (oomycete)

  • live in pretend harmony
  • fewer cell wall-degrading enzymes than non-biotrophs
  • evade detection and avoid elicitation of defence responses
95
Q

Necrotrophic

A

kill plant cells and then feed
e.g. botrytis cinerea (fungus)

  • ‘smash and grab’
  • produce toxins and cell wall-degrading enzymes
96
Q

Hemibiotrophic

A

initially biotrophic and then become necrotrophic

e.g. pseudomonas syringae (bacteria)

97
Q

Plant viruses and agriculture

A
  • difficult to control
  • the more we know, the more we can prevent
  • also in tubers
  • some plant resistance but not enough
  • viruses are biotrophs so need to plan their survival
  • can be DNA or RNA but usually RNA
  • if they kill cells they can’t reproduce
98
Q

Do eukaryotes and bacteria have double or single stranded DNA?

A

double

99
Q

Viral genomes can be

A
  • dsDNA
  • ssDNA (circular)
  • dsRNA
  • ssRNA(+ or - strand)
  • most plant viruses have RNA genomes
  • viruses replicate using plant cell materials and machinery
100
Q

Virus transmission by aphids

A
  • aphids = vectors

- aphids feed on phloem sap using a stylet that they inject into the plants veins

101
Q

Bacterial pathogens: bacterial speck

A
  • pseudomonas

- hemibiotrophs

102
Q

Bacterial pathogens: crown gall

A
  • agrobacterium
  • tDNA integrated into plant chromosome
  • biotroph
103
Q

Bacterial pathogens: blackleg

A
  • erwina
  • plant cell wall degraded by enzymes
  • necrotroph
104
Q

Bacterial pathogens: bacterial wilt

A
  • exopolysaccharide in xylem

- necrotroph

105
Q

Plant pathogenic nematodes

A
  • globally plant parasitic nematodes cause well over $100 billion in crop losses annually
  • very abundant
  • infect root system
106
Q

Root-knot nematode

A

induce expansion in five to seven neighbouring cells to produce giant cells

107
Q

Cyst nematode

A

partially dissolve cell walls between cells to produce a syncytium

108
Q

Nematode effectors

A

modify root cells to become specilised feeding cells

109
Q

Rice blast fungus - magnaporthe oryzae

A
  • ascomycete fungus
  • major disease of rice
  • 10-50% losses of the rice crop
  • air-borne disease
  • infects foliar (leaf) tissue
  • biotrophic
110
Q

Rice blst fungus lifecycle

A
  • sympodial conidia
  • conidium falls and spore tip mucilage is secreted
  • germling with extracellular matrix
  • autophagy occurs and melanizes appressorium
  • penetration peg, pit fields and membrane caps on invasive hypha form
111
Q

Late blight - phytophthora infestans

A
  • most serious potato disease
  • costing 7 billion euros per year
  • threat to global food security
  • genetic resistance readily defeated
  • up to 20 chemical sprays banned/reducing chemicals
  • hemibiotroph
112
Q

Fungal and oomycete hemibiotrophs

A
  • usually make haustoria

- haustoria remain outside the plant plasma membrane and are specialised for nutrient and signal exchange

113
Q

Haustorial mother cell

A

flat structure on cell surface which enters cell to form a bulb-like structure (haustorium)

114
Q

Infection hypha

A

haustorial mother cell on surface enters cell in a rod-like structure

115
Q

arbuscule

A

hand-like structure from haustorial mother cell