Plant Slide Preparation (Lab, Exercise 1)

Question 1

Type of mount where the specimen is mounted on the slide with our without coverslip. It is quick and easy to make but it dries out live sample.

Answer 1

Dry Mount

Question 1

Type of mount where the specimen is mounted on the slide with 1-3 drops of water, with coverslip. It is useful for viewing cellular movement, aquatic organisms, etc.

Answer 1

Wet Mount

Question 2

Type of mount where the specimen from a semiviscous/viscous medium is spread thin across the slide. Allows cells to be viewed
separately, distinguish
cellular debris, organic and
non-organic matter

Answer 2

Smear Slide

Question 3

Type of mount where the specimen is thinly sliced
using a blade

Answer 3

Free-Hand Sectioning

Question 4

Type of mount where the specimen sections are
arranged in sequential
order and were cut using a
microtome

Answer 4

Serial Sectioning

Question 5

Type of mount where a thinly-sliced soft specimen is
placed on the slide and
gently pressed down by the
coverslip. Useful for viewing minuteparts of a macroscopic
structure

Answer 5

Squash Slide

Question 6

Fixating reagent used

Answer 6

Farmer’s fluid (3:1 ethanol to acetic acid)

Question 7

Reagent used to further fix the sample if it is not to be used immediately

Answer 7

70% aclohol

Question 8

Reagent used to dissolve the pectic substance of the middle lamella

Answer 8

1N HCl

Question 9

Reagent that softens and swell tissues, making it easier to spread cells or chromosomes on slides. Also acts as a fixative

Answer 9

45% Glacial Acetic Acid

Question 10

Staining reagent for nucleus/chromosomes

Answer 10

Acetocarmine

Question 11

Seals the cover slip on the slide

Answer 11

Paraffin