Plant stuff Flashcards

(167 cards)

1
Q

5 key traits that appear in all plants but are absent from charophytes?

A
  1. Alternation of generations
  2. Multicellular, dependent embryos
  3. Walled spores produced in sporangia
  4. Multicellular gametangia –multicelluar organs
    that produce gametes
  5. Apical meristems
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2
Q

Alteration of generations

A

Unlike animals in plants after meiosis the
haploid cells can develop into independent
organisms rather than gametes

Alternation of generations:

  • Gametophytes (haploid)
  • Sporophytes (diploid)
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3
Q

Where does meiosis occur in plants?

A

In meiosis, sexual cell division, one diploid (2n) meiocyte (a.k.a. germline cell) divides to produce four haploid (n) daughter cells.
These are further processed to become sex cells (gametes).

In plants this occurs in the archegonia in females and in the antheridia in males.

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4
Q

In plants, walled spores are produced by sporangia

A

 Plant spores are haploid reproductive cells that grow into gametophytes by mitosis.
 Sporopollenin makes the walls of spores very tough and resistant to harsh environments.
 Multicellular organs called sporangia are found on the sporophyte and produce spores.
○ Within sporangia, diploid cells called sporocytes undergo meiosis and generate haploid
spores.
 The outer tissues of the sporangium protect the developing spores until they are ready to be released into the air

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5
Q

Plant gametophytes produce gametes within multicellular organs called gametangia.

A

A female gametangium, called an archegonium, produces a single egg cell in a vase-shaped
organ.
○ The egg is retained within the base.
 Male gametangia, called antheridia, produce and release sperm into the environment.
 In many major groups of living plants, the sperm have flagella and swim to the eggs though a
water film.
Each egg is fertilized within an archegonium, where the zygote develops into the embryo.
 The gametophytes of seed plants are so reduced in size that archegonia and antheridia have
been lost in some lineages.

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6
Q

What is a gymnosperm?

A

Gymnosperms are called “naked seed” plants because their seeds are not enclosed in
chambers.

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7
Q

What is an angiosperm?

A

Angiosperm seeds develop inside chambers called ovaries, which originate within
flowers and mature into seeds.

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8
Q

Walled Spores Produced in Sporangia

A

The sporophyte produces spores in organs
called sporangia.

Spore walls contain sporopollenin, which makes
them resistant to harsh environments

Plant spores are haploid reproductive cells that
can grow into multicellular haploid gametophytes
by mitosis.

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9
Q

Multicellular Gametangia

A

Gametes are produced within organs called
gametangia
• Female gametangia, called archegonia, produce
eggs and are the site of fertilization
• Male gametangia, called antheridia, produce
and release sperm

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10
Q

Moss life cycle

A

A spore germinates into a gametophyte
composed of a protonema and gamete-producing gametophore
• The height of gametophytes is constrained by
lack of vascular tissues
• Rhizoids anchor gametophytes to substrate
• Mature gametophytes produce flagellated
sperm in antheridia and an egg in each
archegonium
• Sperm swim through a film of water to reach
and fertilize the egg

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11
Q

The Ecological and Economic

Importance of Mosses

A

Mosses are capable of inhabiting diverse and
sometimes extreme environments, but are
especially common in moist forests and wetlands
• Some mosses might help retain nitrogen in the
soil
• Many mosses can exist in very cold or dry
habitats because they are able to lose most of
their body water and then rehydrate and
reactivate their cells when moisture again
becomes available.

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12
Q

What are the characteristics of vascular plants?

A

Life cycles with dominant sporophytes
 Vascular tissues called xylem and phloem
 Well-developed roots and leaves

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13
Q

Transport in Xylem and Phloem

A

Vascular plants have two types of vascular tissue: xylem and
phloem
• Xylem conducts most of the water and minerals and includes
dead cells called tracheids
• Water-conducting cells are strengthened by lignin and
provide structural support
• Phloem consists of living cells and distributes sugars, amino
acids, and other organic products
• Vascular tissue allowed for increased height, which provided
an evolutionary advantage

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14
Q

Sporophylls and Spore Variations

A

Milestone in the evolution of plants was the
emergence of sporophylls – modified leaves that
bear sporangia
• Sori are clusters of sporangia on the undersides of
sporophylls
• Strobili are cone-like structures formed from groups
of sporophylls

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15
Q

Heterospory: The Rule Among Seed Plants

A

A heterosporous species produces two kinds of spores.

  • megaspores, which develop into female gametophytes.
  • microspores, which develop into male gametophytes.
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16
Q

Advantages of Reduced Gametophytes

A

The gametophytes of seed plants are microscopic
• The gametophytes of seed plants develop within the
walls of spores that are retained within tissues of the
parent sporophyte
• This arrangement protects the developing
gametophyte from environmental stress and enables
it to obtain nutrients from the sporophyte

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17
Q

Pollen and Production of Sperm

A

Microspores develop into pollen grains, which contain the male
gametophytes
• Pollination is the transfer of pollen to the part of a seed plant
containing the ovules – contrast with bryophytes and seedless v
plants
• Pollen eliminates the need for a film of water and can be
dispersed great distances by air or animals
• If a pollen grain germinates, it gives rise to a pollen tube that
discharges sperm into the female gametophyte within the ovule

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18
Q

Seeds provide some evolutionary advantages over

spores

A

Spores are single celled, seeds are multicelled.
– They may remain dormant for days to years, until
conditions are favorable for germination
– Seeds have a supply of stored food
– They may be transported long distances by wind or
animals

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19
Q

The Angiosperm Life Cycle

A

The flower of the sporophyte is composed of both male and
female structures
• Male gametophytes are contained within pollen grains
produced by the microsporangia of anthers
• The female gametophyte, or embryo sac, develops within an
ovule contained within an ovary at the base of a stigma
• Most flowers have mechanisms to ensure cross-pollination
between flowers from different plants of the same species

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20
Q

Development of Male Gametophytes

in Pollen Grains

A

Pollen develops from microspores within
the microsporangia, or pollen sacs, of anthers
• Each microspore undergoes mitosis to produce two cells: the
generative cell and the tube cell
• A pollen grain consists of the two-celled male gametophyte and
the spore wall
• If pollination succeeds, a pollen grain produces a pollen tube
that grows down into the ovary and discharges two sperm cells
near the embryo sac

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21
Q

Development of Female

Gametophytes (Embryo Sacs)

A

The embryo sac, or female gametophyte, develops within the
ovule
• Within an ovule, two integuments surround a megasporangium
• One cell in the megasporangium undergoes meiosis,
producing four megaspores, only one of which survives
• The megaspore divides, producing a cell partitioned into a
multicellular female gametophyte, the embryo sac

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22
Q

Double Fertilization

A

One sperm fertilizes the egg, while the other combines with two
nuclei in the central cell of the female gametophyte and initiates
development of food-storing endosperm
• The triploid endosperm nourishes the developing embryo
• Within a seed, the embryo consists of a root and two seed
leaves called cotyledons

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23
Q

Fruit Form and Function

A

A fruit develops from the ovary
• It protects the enclosed seeds and aids in seed
dispersal by wind or animals
• A fruit may be classified as dry, if the ovary dries
out at maturity, or fleshy, if the ovary becomes
thick, soft, and sweet at maturity

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24
Q

Fruits are also classified by their development

A

– Simple, a single or several fused carpels
– Aggregate, a single flower with multiple separate
carpels
– Multiple, a group of flowers called an
inflorescence

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25
What are the 2 angiosperm groups?
Monocots (one cotyledon) |  Eudicots (two dicots)
26
Different Whorl Combos
Whorl 1=sepal, A genes Whorl 2=petal, A+B genes Whorl 3=stamen, B+C genes Whorl 4=carpel, C genes
27
Flowering time is regulated by coordinated | genetic changes that respond to:
Photoperiod - Temperature - Plant hormone signals
28
The 2 Processes of Photosynthesis
The light reactions (photo) convert solar energy to chemical energy. ○ The Calvin cycle (synthesis) uses energy from the light reactions to incorporate CO2 from the atmosphere into sugar.
29
What is an Autotroph?
Autotrophs sustain themselves without eating anything derived from other organisms • Autotrophs are the producers of the biosphere, producing organic molecules from CO2 and other inorganic molecules • Almost all plants are photoautotrophs, using the energy of sunlight to make organic molecules
30
What is the reaction for photosynthesis?
6CO2 + 12H2O --> C6H12O6 + 6O2 + 6H2O
31
The light reactions (in the thylakoids)
``` Split H2O – Release O2 – Reduce NADP+ to NADPH – Generate ATP from ADP by photophosphorylation ```
32
Info on Calvin cycle
The Calvin cycle (in the stroma) forms sugar from CO2, using ATP and NADPH. The Calvin cycle begins with carbon fixation, incorporating CO2 into organic molecules
33
What is a photosystem?
``` A photosystem consists of a reaction -center complex (a type of protein complex) surrounded by light -harvesting complexes • The light -harvesting complexes (pigment molecules bound to proteins) transfer the energy of photons to the reaction center ```
34
2 Types of photosystems
Photosystem II (PS II) functions first (the numbers reflect order of discovery) and is best at absorbing a wavelength of 680 nm • The reaction-center chlorophyll a of PS II is called P680 • Photosystem I (PS I) is best at absorbing a wavelength of 700 nm
35
What is linear electron flow?
Linear electron flow, the primary pathway, involves both photosystems and produces ATP and NADPH using light energy • This is the ‘canonical’ process of the lightdependent reactions
36
What is Cyclic electron flow?
``` Cyclic electron flow uses only photosystem I and produces ATP, but not NADPH • No oxygen is released • Cyclic electron flow generates surplus ATP, satisfying the higher demand in the Calvin cycle ```
37
What are C4 plants?
C4 plants minimize the cost of photorespiration by incorporating CO2 into four-carbon compounds in mesophyll cells • This step requires the enzyme PEP carboxylase • PEP carboxylase has a higher affinity for CO2 than rubisco does; it can fix CO2 even when CO2 concentrations are low; O2 does not compete. • These four-carbon compounds are exported to bundle-sheath cells, where they release CO2 that is then used in the Calvin cycle
38
C4 photosynthesis
Primary CO2 fixing enzyme: PEP carboxylase (phosphoenol pyruvate carboxylase) which has higher affinity for CO2 (in fact, almost no affinity for O2) • Needs bundle sheath anatomy • Advantageous in high light, high temperature, high evaporation conditions But requires high energy
39
CAM Plants
Some plants, including succulents, use crassulacean acid metabolism (CAM) to fix carbon • CAM plants open their stomata at night, incorporating CO2 into organic acids • Stomata close during the day, and CO2 is released from organic acids and used in the Calvin cycle
40
CAM Plants photosynthesis
Primary CO2 fixing enzyme: PEP carboxylase as in C4 • Separates CO2 uptake and temporary storage (during night) from final CO2 fixation (during day) • Survival mechanism in arid regions (eg deserts) • High ‘water use efficiency’ (ratio of CO2 fixed to water lost)
41
What are the functions of a root?
– Anchoring the plant – Absorbing minerals and water (from the soil) – Storing carbohydrates (from the leaves)
42
Eudicots and gymnosperm root system
Most eudicots and gymnosperms have a taproot system, which consists of: – A taproot, the main vertical root – Lateral roots, or branch roots, that arise from the taproot
43
Monocot root system
Most monocots have a fibrous root system, which consists of: – Adventitious roots that arise from stems or leaves – Lateral roots that arise from the adventitious roots
44
What are the 5 types of modified roots?
- Prop roots - Storage roots - Strangling/Aerial roots - Buttress roots - Pneumatophores
45
What are the 3 Meristematic tissues of a plant?
Dermal: epidermis, periderm Vascular: xylem, phloem Ground: pith and cortex
46
What is Dermal tissue?
In nonwoody plants, the dermal tissue system consists of the epidermis • A waxy coating called the cuticle helps prevent water loss from the epidermis • Trichomes are outgrowths of the shoot epidermis and can help with insect defense
47
Ground tissue
Tissues that are neither dermal nor vascular are the ground tissue system • Ground tissue internal to the vascular tissue is pith; ground tissue external to the vascular tissue is cortex • Ground tissue includes cells specialized for storage, photosynthesis, and support
48
Vascular tissue
The vascular tissue system carries out long-distance transport of materials between roots and shoots • The two vascular tissues are xylem and phloem • Xylem conveys water and dissolved minerals upward from roots into the shoots • Phloem transports organic nutrients from where they are made to where they are needed
49
Parenchyma Cells
``` – Have thin and flexible primary walls – Lack secondary walls – Large vacuole – Are the least specialized – Perform the most metabolic functions – Retain the ability to divide and differentiate – Can regenerate ```
50
Collenchyma Cells
Collenchyma cells are grouped in strands and help support young parts of the plant shoot • They have thicker and uneven cell walls • They lack secondary walls • These cells provide flexible support without restraining growth
51
Sclerenchyma Cells
Sclerenchyma cells are rigid because of thick secondary walls strengthened with lignin • They are dead at functional maturity • There are two types: – Sclereids are short and irregular in shape and have thick lignified secondary walls – Fibers are long and slender and arranged in threads
52
2 types of plant growth.
Primary for growth in height | Secondary for growth in diameter
53
Water loss from plants
Plants take up large quantities of water • > 90% of the water taken in by roots is lost from the plant as water vapour. • Very little is ‘used’ in any biochemical process • The loss of water from the plant is termed transpiration • Terrestrial plants lose water as an unavoidable consequence of having to take up CO2 • Dehydration is always a risk • But transpiration stream does distribute minerals
54
What are the 3 transport routes between cells?
Three transport routes for water and solutes are – The apoplastic route, through cell walls and extracellular spaces – The symplastic route, through the cytosol, on the inside – The transmembrane route, across cell walls
55
Short-Distance Transport of Solutes | Across Plasma Membranes
Plasma membrane permeability controls short-distance movement of substances • Both active and passive transport occur in plants • In plants, membrane potential is established through pumping H by proton pumps
56
Bulk flow
Efficient long distance transport of fluid requires bulk flow, the movement of a fluid driven by pressure • Water and solutes move together through tracheids and vessel elements
57
Water-Conducting Cells of the Xylem
The two types of water-conducting cells, tracheids and vessel elements, are dead at maturity • Tracheids are found in the xylem of all vascular plants • Vessel elements are common to most angiosperms and a few gymnosperms • Vessel elements align end to end to form long micropipes called vessels
58
Bulk Flow Transport via the Xylem
Xylem sap, water and dissolved minerals, is transported from roots to leaves by bulk flow • The transport of xylem sap involves transpiration, the evaporation of water from a plant’s surface • Transpired water is replaced as water travels up from the roots
59
Pulling Xylem Sap: The Cohesion-Tension | Hypothesis
According to the cohesion-tension hypothesis, transpiration and water cohesion pull water from shoots to roots • Xylem sap is normally under negative pressure, or tension
60
How does bulk flow differ from diffusion?
It is driven by differences in pressure potential, not solute potential – It occurs in hollow dead cells, not across the membranes of living cells – It moves the entire solution, not just water or solutes – It is much faster
61
Sugar-Conducting Cells of the Phloem
Sieve-tube elements are alive at functional maturity, though they lack organelles • Sieve plates are the porous end walls that allow fluid to flow between cells along the sieve tube • Each sieve-tube element has a companion cell whose nucleus and ribosomes serve both cells
62
What is water potential?
Water potential is a measurement that combines the effects of solute concentration and pressure • Water potential determines the direction of movement of water • Water flows from regions of higher water potential to regions of lower water potential
63
Role of rhizobacteria
Rhizobacteria can play several roles – Produce hormones that stimulate plant growth – Produce antibiotics that protect roots from disease – Absorb toxic metals or make nutrients more available to roots
64
Nitrogen fixing bacteria
``` Ammonifying bacteria produce NH3 by breaking down nitrogen in proteins and other organic compounds in humus. Nitrogen-fixing bacteria convert N2 into NH3 In the soil, NH3 picks up another H+ to form NH4  (which plants can absorb) • Plants acquire nitrogen mainly in the form of NO3 – • Soil NO3 - formed by two step processes called nitrification. – Nitrifying bacteria oxidize NH3 to nitrite (NO2 – ) then nitrite to nitrate (NO3 – ) (2 different bacteria) • Nitrogen is lost to the atmosphere when denitrifying bacteria convert NO3 – to N2 ```
65
Fungi and Plant Nutrition
Mycorrhizae (fungus roots) are mutualistic associations of fungi and roots • The fungus benefits from a steady supply of sugar from the host plant • The host plant benefits because the fungus increases the surface area for water uptake and mineral absorption • Mycorrhizal fungi also secrete growth factors that stimulate root growth and branching and produce antibiotics that protect the root.
66
Ectomycorrhizae
In ectomycorrhizae, the mycelium of the fungus forms a dense sheath over the surface of the root • These hyphae form a network in the apoplast, but do not penetrate the root cells • Ectomycorrhizae occur in about 10% of plant families including pine, spruce, oak, walnut, birch, willow, and eucalyptus
67
Arbuscular mycorrhizae
in arbuscular mycorrhizae, microscopic fungal hyphae extend into the root • These mycorrhizae penetrate the cell wall but not the plasma membrane to form branched arbuscules within root cells • Hyphae can form arbuscules within cells; these are important sites of nutrient transfer • Arbuscular mycorrhizae occur in about 85% of plant species, including grains and legumes
68
What is an epiphyte?
``` An epiphyte grows on another plant and obtains water and minerals from rain • Epiphytes do not tap into hosts for sustenance ```
69
Macronutrients
Macronutrients – need about 1-20 g kg-1 dry weight – Nitrogen, Potassium Calcium Magnesium, Phopshorus Sulphur
70
Micronutrients
Micronutrients – need about 100 mg g kg-1 dry weight – Chloride Iron Manganese Boron Zinc Copper Nickel Molybdenum – Often enzyme co-factors for occasional processes
71
WHAT IS A BIOME?
A biome is an area of the planet that can be classified according to the plants and animals that live in it. Temperature, soil, and the amount of light and water help determine what life exists in a biome. Differs from an ecosystem; “interaction between living and non-living things in the environment” vs biome; a specific geographic area notable for the species living there. A biome can consist of a number of ecosystems.
72
GENERAL FEATURES OF TERRESTRIAL | BIOMES
major physical features climatic (temp and ppt) Vegetation (and adaptations) Microorganisms, fungi and animals adapted to that particular environment.
73
GENERAL FEATURES OF TERRESTRIAL BIOMES
VERTICAL LAYERING (STRATIFICATION) - Determined by the shapes and sizes of vegetation within the biome. FORESTS Upper canopy > low-tree layer > shrub layer > ground layer of herbaceous plants > forest floor (litter layer) > root layer. Grasslands (similar but less pronounced) Herbaceous layer of grasses and forbs > litter layer > root layer.
74
r- strategists
``` ‘Live fast, die young’ ¡ Quantity ¡ Unstable environments ¡ Earlier maturity ¡ Deciduous ```
75
K- strategists
``` ‘grow slow, die old’ ¡ Quality ¡ Stable environments ¡ Later maturity ¡ Evergreen ```
76
3 Different types of dispersal?
``` Diffusion • gradual movement • several generations • Across suitable terrain ``` ``` Jump • long distance • Short time scale • Across unsuitable terrain ``` ``` Secular • Diffusion over evolutionary time • genetically modified species ```
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What are biotic factors?
Biotic factors that affect the distribution of organisms may include ¡ Disease + Parasitism ¡ Predation; inc herbivory ¡ Competition ¡ Mutualism
78
COMPETITION – ALLELOPATHY
The chemical inhibition of one plant (or other organism) by another, due to the release into the environment of substances acting as germination or growth inhibitors. ¡ Secondary metabolites that are not required for the growth, development and reproduction of the allelopathic plant. e.g. English Laurel, garlic mustard weed, eucalyptus. Applications; ¡ Agriculture – intercropping - crop rotation - insect biocontrol
79
Abiotic factors
``` ¡ Temperature ¡ Water ¡ Oxygen ¡ Salinity ¡ Sunlight ¡ Soil Most abiotic factors vary in space and time. ```
80
ADAPTATIONS TO TEMPERATURE AND | MOISTURE
Species can extend their distributions by local adaptations to limiting environmental variables. ¡ Ecotype – genetic varieties within a single species. How? All differences are phenotypic, seeds transplanted from one place to another will all perform the same as the resident individuals. 2) All differences are genotypic, if seeds are transplanted between areas the seedlings will retain physiology identical to their home site. 3) A combination of phenotypic and genotypic variation causes an intermediate results.
81
RECAP: EVOLUTION AND ARTIFICIAL | SELECTION
Natural populations are shaped by natural selection – Competition for survival and reproduction – Individuals with most advantageous traits are more likely to survive and reproduce ``` Allele frequencies change in population to become better adapted to the environment • Higher survival and reproduction of individuals with specific traits • Highly selective process increasing alleles associated with specific traits ```
82
What is acclimation?
``` individual plants respond to changes in the environment, by altering their physiology or morphology allowing them to survive the new environment. ```
83
Info on polyploid plants
Polyploid (multi-genome) plants are often bigger and so selected for propagation
84
What is natural food?
The food we eat comes from plants already extensively modified from their original form. Even heritage varieties are extensively genetically modified.
85
Phenotype: physical expression of traits
Conventional breeding based on phenotypes. Cross plants, Look in subsequent generations for “best” Repeat Often only one crop per year LONG process
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ISSUES WITH MODERN BREEDING
Crops were typically bred for high yield under optimal growth conditions Loss of genetic variation in other important traits Modern Agriculture involved planting large areas with a single crop (monoculture) Pros: ease of planting, harvesting, and looking after your crop Cons: May lack resilience against disease and other stresses Older and wild varieties of crop plants can be excellent resources for increasing genetic variation (i.e. stress tolerance)
87
Why use GM methods sometimes and molecular breeding others?
Molecular breeding 1. Desired trait must be present in population 2. Genetic resources must be available 3. Plant should be propagated sexually
88
Why use GM methods sometimes and molecular breeding others?
GM 1. Gene can come from any source 2. Genetic resources not required 3. Plant can be propagated vegetatively
89
RECOMBINANT DNA TECHNOLOGY
To work directly with specific genes, scientists prepare well defined DNA segments in multiple identical copies by a process called DNA cloning Plasmids are small, circular DNA molecules that replicate separately from the bacterial chromosome Researchers can insert DNA into a plasmid to produce a recombinant DNA molecule, which contains DNA from two different sources
90
What are plasmids?
Small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently through their own origin of replication Can be manipulated to include foreign genes into bacteria and from there into plants
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STEPS OF PLANT TRANSFORMATION
Propagate plasmid vector in E. coli Isolate plasmid vector from E. coli and introduce foreign gene Amplify by reintroducing to E. coli Isolate engineered binary vector and introduce into Agrobacterium containing modified Ti plasmid Infect plant tissues with engineered Agrobacterium
92
What are the 4 major physical components of climate?
Four major physical components of climate are: Sunlight Temperature Precipitation Wind.
93
HOW DOES C3 PHOTOSYNTHESIS RESPOND TO RISING [CO2]?
INCREASED CARBON GAIN FROM INCREASED RATES OF CARBOXYLATION AND DECREASED RATES OF OXYGENATION AND PHOTORESPIRATION
94
Heterospory: The Rule Among Seed Plants
Homosporous plants produce one kind of spore, which usually produces a bisexual gametophyte • Heterosporous plants produce two types of spores, which develop into either male or female gametophytes • Ferns and other close relatives are homosporous; seed plants are heterosporous
95
What adaptions do seeded plants share?
Have seeds (by definition) • Reduction in gametophyte generation – microscopic • Heterospory – Single large megaspore , many small microspores • Ovules – contain, megaspore, megasporangium, and integuments • Pollen – protected male gametophytes can travel & disperse widely
96
What is Speciation?
The process by which one species splits into two or more species, is at the focal point of evolutionary theory
97
What is micro and macroevolution?
Microevolution consists of changes in allele frequency in a population over time • Macroevolution refers to broad patterns of evolutionary change above the species level – when different habitats occur at the same time with little competition, results in gradual refinement of existing structures for new functions that lead to an advantage in these different habitats
98
What are the 4 types of modified leaves?
A flower is a specialized shoot with up to four types of modified leaves – floral organs  Sepals, which enclose the flower  Petals, which are brightly colored and attract pollinators  Stamens, which produce pollen  Carpels, which produce ovules • A stamen consists of a stalk called a filament, with a sac called an anther where the pollen is produced • A carpel consists of an ovary at the base and a style leading up to a stigma, where pollen is received
99
Development of Male Gametophytes | in Pollen Grains
Pollen develops from microspores within the microsporangia, or pollen sacs, of anthers • Each microspore undergoes mitosis to produce two cells: the generative cell and the tube cell • A pollen grain consists of the two-celled male gametophyte and the spore wall • If pollination succeeds, a pollen grain produces a pollen tube that grows down into the ovary and discharges two sperm cells near the embryo sac
100
Development of Female | Gametophytes (Embryo Sacs)
The embryo sac, or female gametophyte, develops within the ovule • Within an ovule, two integuments surround a megasporangium • One cell in the megasporangium undergoes meiosis, producing four megaspores, only one of which survives • The megaspore divides, producing a cell partitioned into a multicellular female gametophyte, the embryo sac
101
POOLS OF STEM CELLS RECEIVE SIGNALS THAT PROMOTE THE | DEVELOPMENT OF SPECIFIC TISSUES
``` Plants produce new organs via meristems • Meristems are pools of stem cells that can be activated to produce specific tissue types. • The shoot apical meristem produces vegetative growth • The inflorescence and floral meristems produce the reproductive structures that will ultimately become flowers. • These transitions are promoted by genes encoding transcription factors (TFs). ```
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What are Phytochrome | Photoreceptors?
``` Phytochromes are pigments that regulate many of a plant’s responses to light throughout its life • These responses include seed germination and flowering ```
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Long and short day plants
Some processes, including flowering in many species, require a certain photoperiod • Plants that flower when a light period is shorter than a critical length are called short-day plants • Plants that flower when a light period is longer than a certain number of hours are called long-day plants • Flowering in day-neutral plants is controlled by plant maturity, not photoperiod
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How is flowering time regulated?
``` Flowering time is regulated by coordinated genetic changes that respond to: - Photoperiod - Temperature - Plant hormone signals ```
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Linear Electron Flow
During the light reactions, there are two possible routes for electron flow: cyclic and linear • Linear electron flow, the primary pathway, involves both photosystems and produces ATP and NADPH using light energy • This is the ‘canonical’ process of the lightdependent reactions
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Carbon reactions in the stroma
Carbon enters the cycle as CO2 and leaves as a sugar named glyceraldehyde 3-phospate (G3P) • For net synthesis of 1 G3P, the cycle must take place three times, fixing 3 molecules of CO2 • The Calvin cycle has three phases – Carboxylation/ Carbon fixation (catalyzed by rubisco) – Reduction – Regeneration of the CO2 acceptor (RuBP)
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C4 Plants
C4 plants minimize the cost of photorespiration by incorporating CO2 into four-carbon compounds in mesophyll cells • This step requires the enzyme PEP carboxylase • PEP carboxylase has a higher affinity for CO2 than rubisco does; it can fix CO2 even when CO2 concentrations are low; O2 does not compete. • These four-carbon compounds are exported to bundle-sheath cells, where they release CO2 that is then used in the Calvin cycle
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Dermal tissue
In nonwoody plants, the dermal tissue system consists of the epidermis • A waxy coating called the cuticle helps prevent water loss from the epidermis • Trichomes are outgrowths of the shoot epidermis and can help with insect defense
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Ground tissue
Tissues that are neither dermal nor vascular are the ground tissue system • Ground tissue internal to the vascular tissue is pith; ground tissue external to the vascular tissue is cortex • Ground tissue includes cells specialized for storage, photosynthesis, and support
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Water loss from plants
Plants take up large quantities of water • > 90% of the water taken in by roots is lost from the plant as water vapour. • Very little is ‘used’ in any biochemical process • The loss of water from the plant is termed transpiration • Terrestrial plants lose water as an unavoidable consequence of having to take up CO2 • Dehydration is always a risk • But transpiration stream does distribute minerals
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What are the 3 transport roots for water and solutes in plant cells?
Three transport routes for water and solutes are – The apoplastic route, through cell walls and extracellular spaces – The symplastic route, through the cytosol – The transmembrane route, across cell walls
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Short-Distance Transport of Solutes | Across Plasma Membranes
Plasma membrane permeability controls short-distance movement of substances • Both active and passive transport occur in plants • In plants, membrane potential is established through pumping H by proton pumps
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Short-Distance Transport of Water Across | Plasma Membranes
• To survive, plants must balance water uptake and loss • Osmosis determines the net uptake or water loss by a cell and is affected by solute concentration and pressure • Water potential is a measurement that combines the effects of solute concentration and pressure • Water potential determines the direction of movement of water • Water flows from regions of higher water potential to regions of lower water potential
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Water Movement Across Plant Cell Membranes
Water potential affects uptake and loss of water by plant cells • If a flaccid (limp) cell is placed in an environment with a higher solute concentration, the cell will lose water and undergo plasmolysis • Plasmolysis occurs when the protoplast shrinks and pulls away from the cell wall
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how does Bulk flow differ from diffusion?
It is driven by differences in pressure potential, not solute potential – It occurs in hollow dead cells, not across the membranes of living cells – It moves the entire solution, not just water or solutes – It is much faster
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Sugar-Conducting Cells of the Phloem
Sieve-tube elements are alive at functional maturity, though they lack organelles • Sieve plates are the porous end walls that allow fluid to flow between cells along the sieve tube • Each sieve-tube element has a companion cell whose nucleus and ribosomes serve both cells
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Plants adapted to deserts
A xerophyte is a species of plant that has adaptations to survive in an environment with little liquid water. Desert or an ice- or snow-covered region. Types: ¡ 1) Succulents; store water in their stems or leaves (enlargement of vacuoles) e.g. (cacti&euphorbs) ¡ 2) Non-succulent ‘drought endurers’ perrenials‘true xerophytes’. Lose 70% water. Effects growth processes such as cell elongation; usually small and weak. ¡ 3) Ephemerals ‘drought escaping’ – rain > seeds germinate > r-growth > flower and set seed before soil dries.
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What causes temperature differentials?
1) Incoming solar radiation. 2) The distribution of land and sea. Water heats and cools slowly due to high specific heat. Land heats and cools quickly so land controlled (continental) climates have large daily and seasonal variation
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THE EFFECT OF TOPOGRAPHY ON ABIOTIC | FACTORS
``` High latitudes/elevation ¡ Treeline – above which no trees can grow. 1. Lack of soil 2. Desiccation of leaves in cold weather 3. Short growing season 4. Lack of snow, exposing plants to winter drying 5. Excessive snow through the summer 6. Strong winds 7. Rapid heat loss at night 8. Excessive soil temp during the day 9. Drought ```
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SIGNAL TRANSDUCTION
Protein kinase add phosphates to proteins in a process called phosphorylation, ¡ Protein phosphatases rapidly remove the phosphates from proteins, a process called dephosphorylation ¡ This phosphorylation and dephosphorylation system acts as a molecular switch, turning activities on and off or up or down, as required ¡ Many signaling pathways involve second messengers ¡ These are small, nonprotein, water-soluble molecules or ions that spread throughout a cell by diffusion (Calcium ions (Ca2+), cyclic GMP (cGMP), inositol triphosphate (IP3) and diacylglycerol (DAG))
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Genetic Variation makes evolution | possible
Genetic variation among individuals is caused by differences in genes or other DNA segments • Phenotype is the product of inherited genotype and environmental influences • Natural selection can only act on variation with a genetic component • Artificial selection can only act on variation with a genetic component
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Why do we sequence genomes?
Genome sequencing provides: complete gene catalogue for a species, • regulatory elements that control their function • framework for understanding genomic variation Genome sequence is a prerequisite resource for: Understand the roles of genes in plant development and adaptation • Exploit the natural genetic diversity of an organism for plant breeding (artificial selection)
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Types of polyploidy
AUTOPOLYPLOIDS: duplicate genome of same Species Autotetraploid: duplicate genome of same diploid species ALLOPOLYPLOIDS: duplicate genome of different species Allohexaploid: six complete sets of chromosomes derived from different species.
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Seed Dormancy: An Adaptation for Tough Times
Seed dormancy increases the chances that germination will occur at a time and place most advantageous to the seedling • The breaking of seed dormancy often requires environmental cues, such as temperature or lighting changes • Germination depends on imbibition, the uptake of water due to low water potential of the dry seed • The radicle (embryonic root) emerges first • Next, the shoot tip breaks through the soil surface
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Development is promoted by different types of meristems
``` • During transition to reproductive development, the inflorescence meristem is formed. In turn, it leads to production of floral meristems • Both will form the organs of an individual flower. • The inflorescence meristem is indeterminate, meaning that it can keep producing new tissue. • The floral meristem is determinate, meaning that it makes only one set of the specified whorls. ```
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POOLS OF STEM CELLS RECEIVE SIGNALS THAT PROMOTE THE | DEVELOPMENT OF SPECIFIC TISSUES
``` Plants produce new organs via meristems • Meristems are pools of stem cells that can be activated to produce specific tissue types. • The shoot apical meristem produces vegetative growth • The inflorescence and floral meristems produce the reproductive structures that will ultimately become flowers. • These transitions are promoted by genes encoding transcription factors (TFs). ```
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Why is it important to time | flowering?
• It’s critical that plants turn on floral development genes at the right time to initiate flower formation. • It is also critical that plants do NOT turn on those genes before the plant is ready to flower. • If a plant flowers at the wrong time, it may lack the appropriate environmental conditions for successful pollination and seed development.
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Pfr info
Responses to Pfr: seed germination, inhibition of vertical growth and stimulation of branching, setting internal clocks and control of flowering. Phytochromes exist in two photo reversible states, with conversion of Pr to Pfr triggering many developmental responses • Red light triggers the conversion of Pr to Pfr • Far-red light triggers the conversion of Pfr to Pr • The conversion of Pr to Pfr is faster than the reverse process
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BIOLOGICAL CLOCKS - | CIRCADIAN RHYTHMS
``` Circadian rhythms are cycles that are about 24 hours long and are governed by an internal “clock” • These cycles can be free-running, varying from 21 to 27 hours, when organisms are kept in a constant environment • The 24-hour period arises from the transcription of “clock genes” regulated through negative-feedback loops ```
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What is a photoperiod?
``` Photoperiod is detected by leaves, which cue buds to develop as flowers • The flowering signal molecule is called florigen • Florigen may be a protein governed by the FLOWERING LOCUS T (FT) gene • This is a mobile protein moving from the leaf to the meristem ```
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Chloroplasts: The Sites of Photosynthesis in Plants
CO2 enters and O2 exits the leaf through microscopic pores called stomata • The chlorophyll is in the membranes of thylakoids (connected sacs in the chloroplast); thylakoids may be stacked in columns called grana • Chloroplasts also contain stroma, a dense interior fluid
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Carbon reactions in the stroma
Carbon enters the cycle as CO2 and leaves as a sugar named glyceraldehyde 3-phospate (G3P) • For net synthesis of 1 G3P, the cycle must take place three times, fixing 3 molecules of CO2 • The Calvin cycle has three phases – Carboxylation/ Carbon fixation (catalyzed by rubisco) – Reduction – Regeneration of the CO2 acceptor (RuBP)
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Alternative mechanisms of carbon | fixation
On hot, dry days, plants close stomata, which conserves H2O but also limits photosynthesis • The closing of stomata reduces access to CO2 and causes O2 to build up • These conditions favor an apparently wasteful process called photorespiration
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What is Photorespiration?
In most plants (C3 plants), initial fixation of CO2, via rubisco, forms a three-carbon compound (3- phosphoglycerate) • In photorespiration, rubisco adds O2 instead of CO2 in the Calvin cycle, producing a two-carbon compound • Photorespiration consumes O2 and organic ‘fuel’ and releases CO2 without producing ATP or sugar
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What does a stem consist of?
``` A stem is an organ consisting of – An alternating system of nodes, the points at which leaves are attached – Internodes, the stem segments between nodes ```
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Different modified stems
Bulbs: leaf bases and short stem Tubers: swollen underground stems Rhizomes: underground stems Stolons: aboveground horizontal shoots
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Basic organs of a plants
Leaves: – main photosynthetic organ – Leaf blade and petiole Roots: – main organ for water and nutrient uptake – Taproots with laterals in eudicots and gymnosperms – Adventitious roots in monocots (from the stem) – Root hairs for absorption – Also anchorage ``` Stem: – Nodes and internodes – Terminal bud with apical meristem – support and transport tissues – Storage ```
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Secondary growth thickens roots and | shoots
Lateral meristems add thickness to woody plants, a process called secondary growth • There are two lateral meristems: the vascular cambium and the cork cambium • The vascular cambium adds layers of vascular tissue called secondary xylem (wood) and secondary phloem • The cork cambium replaces the epidermis with periderm, which is thicker and tougher Secondary growth is characteristic of gymnosperms and many eudicots, but not monocots
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Short-Distance Transport of Solutes | Across Plasma Membranes
Plasma membrane permeability controls short-distance movement of substances • Both active and passive transport occur in plants • In plants, membrane potential is established through pumping H by proton pumps
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Long-Distance Transport: The Role of Bulk | Flow
Efficient long distance transport of fluid requires bulk flow, the movement of a fluid driven by pressure • Water and solutes move together through tracheids and vessel elements of xylem, and sieve-tube elements of phloem – Water primarily through Xylem – Solutes primarily through Phloem
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Water-Conducting Cells of the Xylem
The two types of water-conducting cells, tracheids and vessel elements, are dead at maturity • Tracheids are found in the xylem of all vascular plants • Vessel elements are common to most angiosperms and a few gymnosperms • Vessel elements align end to end to form long micropipes called vessels
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Sugar-Conducting Cells of the Phloem
Sieve-tube elements are alive at functional maturity, though they lack organelles • Sieve plates are the porous end walls that allow fluid to flow between cells along the sieve tube • Each sieve-tube element has a companion cell whose nucleus and ribosomes serve both cells Depending on the species, sugar may move by symplastic (passing through plasmodesmata)or both symplastic and apoplastic pathways • Companion cells enhance solute movement between the apoplast and symplast • In many plants, phloem loading requires active transport • Proton pumping and cotransport of sucrose and H+ enable the cells to accumulate sucrose • At the sink, sugar molecules diffuse from the phloem to sink tissues and are followed by water
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GENERAL FEATURES OF TERRESTRIAL | BIOMES
1) major physical features 2) climatic (temp and ppt) 3) Vegetation (and adaptations) 4) Microorganisms, fungi and animals adapted to that particular environment.
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What is convergent evolution?
organisms share similar characteristics as they undergo natural selection independently, but in similar environments
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r and K strategies in plants
``` r- strategists ¡ ‘Live fast, die young’ ¡ Quantity ¡ Unstable environments ¡ Earlier maturity ¡ Deciduous ``` ``` K- strategists ¡ ‘grow slow, die old’ ¡ Quality ¡ Stable environments ¡ Later maturity ¡ Evergreen ```
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Role of nutrients
* Structural components * Metabolic Components * Enzyme activators * Osmosis * Cell permeability/membrane functioning
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Mineral nutrient availability
Depends on soil characteristics: pH, the size and composition of soil particles • Most soil particles are negatively charged • Rainfall leaches out the –ve anions, such as NO3 -, PO4 - , SO4 2- • Cations held more strongly: K+ , Ca2+, Mg2+
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Plant nutrition often involves relationships | with other organisms
Plants and soil microbes have a mutualistic relationship – Dead plants provide energy needed by soildwelling microorganisms – Secretions from living roots support a wide variety of microbes in the near-root environment • The layer of soil bound to the plant’s roots is the rhizosphere • The rhizosphere contains bacteria (Rhizobacteria) that act as decomposers and nitrogen-fixers
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Rhizobacteria
Free-living rhizobacteria thrive in the rhizosphere, and some can enter roots • The rhizosphere has high microbial activity because of sugars, amino acids, and organic acids secreted by roots • Rhizobacteria can play several roles – Produce hormones that stimulate plant growth – Produce antibiotics that protect roots from disease – Absorb toxic metals or make nutrients more available to roots
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Nitrogen metabolism
Ammonifying bacteria produce NH3 by breaking down nitrogen in proteins and other organic compounds in humus. Nitrogen-fixing bacteria convert N2 into NH3 In the soil, NH3 picks up another H+ to form NH4+ (which plants can absorb) • Plants acquire nitrogen mainly in the form of NO3 – • Soil NO3 - formed by two step processes called nitrification. – Nitrifying bacteria oxidize NH3 to nitrite (NO2 –) then nitrite to nitrate (NO3 –) (2 different bacteria) • Nitrogen is lost to the atmosphere when denitrifying bacteria convert NO3 – to N2
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Mycorrhizae info
Mycorrhizae (fungus roots) are mutualistic associations of fungi and roots • The fungus benefits from a steady supply of sugar from the host plant • The host plant benefits because the fungus increases the surface area for water uptake and mineral absorption • Mycorrhizal fungi also secrete growth factors that stimulate root growth and branching and produce antibiotics that protect the root.
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Biotic factors that affect the distribution | of organisms
``` may include: Disease + Parasitism Predation; inc herbivory Competition Mutualism ```
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Adaptation (domestication, breeding) vs acclimation
``` Adaptation: genetic changes in the entire population fixed by natural or artificial selection over many generations. ``` ``` Acclimation: individual plants respond to changes in the environment, by altering their physiology or morphology allowing them to survive the new environment. ```
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SIGNAL TRANSDUCTION
Protein kinase add phosphates to proteins in a process called phosphorylation, ¡ Protein phosphatases rapidly remove the phosphates from proteins, a process called dephosphorylation ¡ This phosphorylation and dephosphorylation system acts as a molecular switch, turning activities on and off or up or down, as required ¡ Many signaling pathways involve second messengers ¡ These are small, nonprotein, water-soluble molecules or ions that spread throughout a cell by diffusion
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ISSUES WITH MODERN BREEDING
Crops were typically bred for high yield under optimal growth conditions Loss of genetic variation in other important traits Modern Agriculture involved planting large areas with a single crop (monoculture) Pros: ease of planting, harvesting, and looking after your crop Cons: May lack resilience against disease and other stresses Older and wild varieties of crop plants can be excellent resources for increasing genetic variation (i.e. stress tolerance)
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HOW DO I GET DNA INTO PLASMID?
Use restriction enzymes to cut plasmid DNA at a specific site Use restriction enzymes to cut plasmid DNA at a specific site Amplify “target” DNA sequence with PCR from other organism, cut with same RE, and ligate Use restriction enzymes to cut plasmid DNA at a specific site Amplify “target” DNA sequence with PCR from other organism, cut with same RE, and ligate Plasmid has maintained ori, will replicate in bacteria
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Exploring global climate patterns
First thing is the warming of the atmosphere to allow for survival of life. Solar radiation hits the earths surface, some is absorbed, some is reflected. And some is trapped in the greenhouse gases that makeup the ozone. Roughly half is absorbed by the earths surface. Ozone largely depleted by CFC’s. But huge prohibition of these in 1987 Montreal Protocol. Phase out use of CFC’s internationally. Hole over Antarctica is decreasing in size. The hole is over Antarctica because conditions must be cool enough to allow for formation of Polar Stratospheric Clouds which requires much cooler temperatures. CFC’s and halons (containing bromine) are produced mostly in the N hemisphere, and are distributed polewards via wind circulation. Formation of PSC promote production of chemically active chlorine and bromine. Leads to rapid ozone loss when sunlight returns in sept/oct. Sun strikes the earth at oblquie angles in the upper and lower regions, but at 90 degree angles at the equator. More diffuse at higher latitudes.
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Atmospheric circulation
At the equator; low pressure, warm moisture rich air rises and then forms clouds as it cools, falls as rain and high storm risk. At 300 N/S; high pressure, dry cool air draws moisture from the soil.
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Ocean currents
Winds - atmospheric circulation. Earth’s rotation – Coriolis force. Changes in water density – temperature and salinity This can be confounded by: Topography of the ocean floor – this can modify the speed and direction of ocean currents. Two types of currents: Surface currents – 10%. At the shore these are affected by wind and tide. In the ocean wind is the major driving force. Deep ocean currents – 90%
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Local moderations of climate
Strong influence of heating and cooling of coastal regions by ocean currents. During a hot day (when the land is warmer than the water), air over the land heats up and rises, drawing cooler air from across the ocean. By contrast, land loses heat faster (such as during cool nights), this causes air above the ocean to rise (because it is now warmer), drawing cooler air from over the land across the ocean.
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Describe three strategies plants can use to conserve water, and explain how they work. Name the ecosystem or biome that each strategy is found in. Explain whether these are the product of plant acclimation or adaptation and justify your choice with reasoning.
Plants in the desert can have a waxy cuticle which stops water molecules from dissipating or being absorbed into the air. Furthermore, stomata cycles are also another important feature for desert plants. This is because they can open their stomata during the night to obtain CO2 for photosynthesis and can then close their stomata during the day to reduces water loss through transpiration. A third and final strategy is that leaves are reduced to spines which reduce the surface area for transpiration. Stomata are a result of acclimation because they are temporarily closed and opened. However, a waxy cuticle and spines are a result of adaptation because they are long term/ permanent and they are a change in physical composition.
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Explain long distance transport of water in plants from the soil to the leaves, including the different tissues and compartment involved. Explain how stomata control the process of water loss through transpiration
Long distance transport of water from the soil to the leaves happens due to root hair cells and roots absorbing the water due to water potential. Moreover, the water is absorbed via the apoplastic and the symplastic route. The water is then taken up in the xylem, where Water and solutes move together through tracheid and vessel elements of xylem. Water is brought up through the xylem because of bulk flow where there is a movement in fluid due to pressure. Some water is lost due to transpiration. Stomata can help reduce water loss by closing during certain conditions such as in arid regions of hot or cold climates where the stomata can open at night to let in CO2, but close during the day to minimize water loss.
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Describe the movement of electrons in photosynthetic electron transport and explain how this process results in the production of ATP and NADPH. Name the three phase of the Calvin cycle and what happens in each phase. Explain the process of photorespiration and under what conditions it might occur.
During the light reactions, there is one possible route for electron flow to produce ATP and NADPH which is linear flow. In linear electron flow, the primary pathway which involves both photosystems and produces ATP and NADPH using light energy. This happens due to a primary electron acceptor in the reaction centre accepting a excited electron from chlorophyll a which takes place at both reaction centres. ATP and NADPH are produced on the side facing the stroma, where the Calvin cycle takes place. The 3 phases of the Calvin cycle are: Carbon fixation, reduction and regeneration. During carbon fixation a CO2 molecule combines with five-carbon acceptor molecules and is catalysed by rubisco. Next is reduction, where NADPH donates electrons to, or reduces, a three-carbon intermediate to make G3P. Finally is regeneration. where Some G3P molecules go to make glucose, while others must be recycled to regenerate the RuBP acceptor. Photorespiration is the process of light-dependent uptake of molecular oxygen (O2) concomitant with release of carbon dioxide (CO2) from organic compounds. The gas exchange resembles respiration and is the reverse of photosynthesis where CO2 is fixed and O2 released. Furthermore, Photorespiration generally occurs on hot, dry, sunny days causing plants to close their stomata and the oxygen (O2) concentration in the leaf to be higher than the carbon dioxide (CO2) concentration.
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What are the 4 main groups of plants and features.
Bryophytes: are considered the simplest of the four types, because they lack a full vascular system, true roots and true leaves. These include mosses and other small plants that grow in wet areas and use spores to reproduce. Pteridophytes: Like ferns, are plants that also use spores to reproduce, however they have evolved a vascular system and so are considered to be more sophisticated than bryophytes. These were the earliest trees on earth; as soon as plants gained vascular systems they were able to grow quite tall as fern trees. They do not have a tap root but have roots that adventitiously sprout from the stem. Gymnosperms: Include plants like conifers and pines. These plants use seeds to reproduce instead of spores, which is an improvement upon the pteridophyte model because seeds are much more durable than spores. This means the genetic information is safer and more likely to be successful. The seeds of gymnosperms are not enclosed in an ovule; they require both male and female cones to reproduce. Male cones are smaller and softer than their female counterparts, and pollination occurs via insects, animals or the wind. Angiosperms are the most sophisticated plants on earth, and include all flowering plants. They can be broken down further into two groups: monocots and dicots.
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Extra info on alteration of generation
Plants alternate between two multicellular stages, a reproductive cycle called alternation of generations • The gametophyte (“gamate-producing plant”) is haploid and produces haploid gametes by mitosis (egg and sperm) • Fusion of the gametes gives rise to the diploid sporophyte, which produces haploid spores by meiosis
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Pollen and Production of Sperm
Microspores develop into pollen grains, which contain the male gametophytes • Pollination is the transfer of pollen to the part of a seed plant containing the ovules – contrast with bryophytes and seedless v plants • Pollen eliminates the need for a film of water and can be dispersed great distances by air or animals • If a pollen grain germinates, it gives rise to a pollen tube that discharges sperm into the female gametophyte within the ovule
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The Life Cycle of a Pine:
Three key features of the gymnosperm life cycle are – Dominance of the sporophyte generation • Miniaturization of their gametophytes – Production/development of seeds from fertilized ovules – The transfer of sperm to ovules by pollen The pine tree is the sporophyte that produces sporangia in male and female cones • Pollen cones are small and consist of modified leaves (microsporophylls) that bear microsporangia • Cells called microsporocytes undergo meiosis to produce haploid microspores inside the microsporangia Ovulate cones are larger and consist of both modified leaves (megasporophylls bearing megasporangia) and modified stem tissue • Within each ovule, megasporocytes undergo meiosis to produce haploid megaspores While pollen tube develops – megasporocytes undergoes meiosis – produces 4 haploid cells – one survives as a megaspore. The megaspore develops into a female gametophyte that contains two or three archegonia – each will form an egg. By the time eggs are mature, sperm cells have developed into pollen tube and fertilization occurs when sperm and egg unite.