Plasmodium Prt. 2

Question 1

Diagnostic points (trophozoite)

• Small, regular, fine to fleshy cytoplasm

• Infected RBCs not enlarged

• Numerous, multiple infection is common

• Ring, comma, marginal or accole forms are seen; often have double chromatin dots

• Maurer’s dots not clearly visible

Answer 1

Plasmodium falciparum (trophozoite stage)

Question 2

• Early trophozoites have the characteristic signet ring shape.

• Also, unique to it is the presence of multiple trophozoites in one cell.

Answer 2

P. falciparum trophozoite

Question 3

Diagnostic points (schizont)

• Small, rarely fill the RBC

• RARE in peripheral blood

• 16-32 or more merozoites arranged in a compact cluster

• Single or dark pigment

• Usually associated with many young ring forms

Answer 3

P. falciparum schizont

Question 4

• The gametocytes of this have a crescent or banana shape

Answer 4

P. falciparum gametocyte

Question 5

Diagnostic points

• Small, compact, central chromatin dot

• Pigments closely adhere to the chromatin

Answer 5

Macrogametocyte

P. falciparum gametocyte

Question 6

Diagnostic points

▪ Broader, shorter and more sausage-shaped

Loosely scattered chromatin

Golden brown pigments scattered at the central half

Answer 6

P. falciparum gametocyte

Microgametocyte

Question 7

DIAGNOSTIC POINTS: (trophozoite)

• Red blood cells infected by this are often larger than uninfected red blood cells

• They are approximately 1.5 times the size of a normal cell

Answer 7

P. vivax trophozoite

Question 8

DIAGNOSTIC POINTS: (trophozoite)

• Irregular or fragmented cytoplasm (amoeboid)

• Mature ring forms tend to be large and coarse

• Schuffner’s dots are frequently visible

Answer 8

P. vivax trophozoite

Question 9

• A schizont showing the large number of merozoites typical of this species (16-24)

• Also note the larger size compared to a normal red blood cell

Answer 9

P. vivax schizont

Question 10

• Characteristic trophozoites of this showing the ring shape and the tendency of infected cells to be of normal or smaller size (arrows)

Answer 10

P. malariae trophozoite

Question 11

• A schizont containing merozoites (6 to 12) giving a coarse granular appearance

Answer 11

P. malariae schizont

Question 12

Shuffner’s dots

• A red blood cell showing the Schuffner’s dots characteristic of cells infected by

Answer 12

Plasmodium vivax
Plasmodium ovale.

Question 13

Diagnosis

• Thick and thin smear STAINS

Answer 13

-Giemsa stain (BEST STAIN)
-Acridine orange staining

Question 14

Diagnosis

Answer 14

Thick and thin smear
Serologic tests
Antigen or DNA Probe Detection Method

Question 15

Diagnosis

Serologic Tests

Answer 15

-Indirect Fluorescent Antibody
-ELISA (enzyme-linked immunosorbent assay)

Question 16

Diagnosis

Antigen or DNA Probe Detection Method -Immunocapture Assay: detection of Plasmodium specific______

Answer 16

lactate dehydrogenase

Question 17

• based on the detection of histidine-rich P. falciparum antigen in whole blood

Answer 17

Dipstick Tests

Question 18

Dipstick Tests

Answer 18

Parasite-F test (Beckton Dickinson)

ICT Malaria Pf test (ICT Diagnostics)

Question 19

-may give false (+) result in rheumatoid factor

Answer 19

Parasite-F test (Beckton Dickinson)

Question 20

Vivax

A schizont showing the large number of merozoites typical of this species

Answer 20

(16-24)

Question 21

Malariae

A schizont containing merozoites giving a coarse granular appearance

Answer 21

(6 to 12)

Question 22

Falciparum

or more merozoites arranged in a compact cluster

Answer 22

16-32