PMTP_Serum and Plasma Preparation

Question 1

Normal values for RBC is _______ while 150-300 microliter for _______.

Answer 1

4.5-6 million, thrombocytes

Question 2

True or False.
Blood is about 10% of the total body weight.

Answer 2

False

Question 3

Coagulation testing uses blue top tube and contains _____.

Answer 3

serum

Question 4

True or False.
Plasma is recommended for serology tests.

Answer 4

False

Question 5

Plasma is the clear, straw-colored liquid portion of the blood and collected in an ___________ tube.

Answer 5

anticoagulant tube (heparin or fluoride)

Question 6

Plasma proteins include albumin, _______, _______ and others.

Answer 6

globulin and fibrinogen

Question 7

A clear liquid with 90% water that can be separated from clotted blood and has no fibrinogen and other clotting factors.

Answer 7

Serum

Question 8

Serum is collected using a plain tube with ____ or ____ or ____ top with no anticoagulant.

Answer 8

red, gold, yellow

Question 9

In preparation of serum, allow the blood to clot by leaving it undistributed at room temperature which usually takes 15-30 minutes but not longer than _____.

Answer 9

1 hour

Question 10

True or False.
Both plasma and serum is centrifuged for 15 minutes at 2000-2500 rpm.

Answer 10

True

Question 11

The relative centrifugal force is determined by ______ and ______ and expressed as gravity.

Answer 11

RPM and Radius of the Rotor Head

Question 12

True or False.
Most laboratory specimens are centrifuged for 15 minutes at 2000-2500 g.

Answer 12

False

Question 13

The method of dividing or separating specimens into separate containers.

Answer 13

Aliquoting

Question 14

True or False.
Specimens should be separated within two hours or less after collection.

Answer 14

True

Question 15

In coagulation studies, ______ and ____ blood tests are the most affected since it could increase if the collected blood is allowed to sit for longer than two hours.

Answer 15

potassium and glucose

Question 16

Conveniently load the test tubes in the centrifuge _____ to each other.

Answer 16

opposite

Question 17

Transfer the liquid serum/plasma intro another test tube using the ________.

Answer 17

Pasteur pipette

Question 18

True or False.
Label the transfer tube in which the aliquot of serum/plasma is placed after the aliquot is added to the tube.

Answer 18

False

Question 19

______ serum sample is characterized by intensely yellow serum sample due to elevated bilirubin level of greater than _______.

Answer 19

Icteric serum, 430 um or 25 mg/L

Question 20

True or False.
Fragility Testing is conducted to detect whether white blood cells are more likely to break down.

Answer 20

False

Question 21

Hemolyzed sample is characterized by the leakage of intracellular substances and bursting of _____.

Answer 21

RBC

Question 22

Lipemic serum sample is characterized by ______ or ______ serum which appears when the TAG level reaches _____.

Answer 22

milky or highly turbid, 4.6 mm or 4 g/L