post lab quiz ex 13 & 14

Question 1

The test performed in the picture above is the oxidase test and a positive result demonstrates the presence of

window is purple

Answer 1

cytochrome C

Question 2

the image is the catalase test and the result is

bubble on the slide

Answer 2

postive

Question 3

The bacteria in this cardinal group do not use oxygen, but the bacteria are NOT killed by oxygen either. These organisms are called

Answer 3

aerotolerant anaerobes

Question 4

The enzyme _______________ converts H2O2 (hydrogen peroxide) to H2O and O2.

Answer 4

catalase

Question 5

The bacteria in this cardinal group prefer to use oxygen, but can perform fermentation as well. These organisms are called ________________

Answer 5

Facultative anaerobes

Question 6

The bacteria in this cardinal group are killed by oxygen. These organisms are called ________________

Answer 6

Obligate anaerobes

Question 7

You inoculated nitrate broth with an unknown and incubate at 37 degrees for 48 hrs. You are ready to test for nitrate reduction. After adding reagents A and B, the broth does not change color. Then you add zinc which causes the tube to turn a pinkish red color.

A. Is this a positive or negative result for nitrate reduction?

B. What does the zinc detect that helps you answer the previous question?

Answer 7

A. negative
B. Zinc detects the presence of nitrate by turning red or pink. If the tube is a reddish color after adding zinc then the bacteria species is negative for nitrate reduction.

Question 8

The enzyme _______________ converts O2- + 2H+ to H2O2 (hydrogen peroxide) + O2.

Answer 8

superoxide dismutase