PRAC 1 Flashcards
(11 cards)
What are the 3 types of microscopes and their roles
- Light Microscope- light passes thru the specimen
- SEM- uses electron beam to form a surface image
- TEM- where electrons from a beam pass thru a thin specimen
What does the high vs low magnifying observe
High observes cells and cellular organelles via light transmitted thru a specimen
Low observes the outside components of organisms via light reflected off a specimen Low observes
What are the parts of a compound microscope
- Diopter adjustment ring
- Binocular observation tube
- Observing tube clamping knob
- Revolving nosepiece
- Body
- Coarse adjustment knob
- Fine adjustment knob
- Power switch
- Light Intensity
- Illuminator
- Base
- Condenser (above) Iris Diaphragm (below)
- Stage
- Specimen holder
- Objective
- Eyepiece
What are the functions of each part of the microscope: base, body, arm, revolving nose piece, eyepiece, objective lenses, stage, condenser lens, iris diaphragm, light (illuminatior), mechanical, coarse, fine and slide adjustment knob
Base: supports microscope
Body: houses prisms, eyepiece and lenses
arm: supports body tube and lenses
revolving nose piece: contains objective lenses and rotates the objectives
eyepiece: what you look thru
objective lenses: the different magnification
stage: where slide is placed
condenser lens: concentrates light
iris diaphragm: regulates amount of light
light (illuminatior): light source
mechanical stage: moves stage around
coarse: rapidly focuses slide
fine: slowly focuses slide
slide adjustment knob: moves slide up/down and side to side
What is each magnification and their total magnification
Low (red): x4 (x40)
Medium (yellow): x10 (x100)
High (blue): x40 (x400)
What is the equation uses to calculate the size of specimen (mm)
Field of view (mm) / # times of specimen fits across the view
What is the approximate FOV for each objective
4x: 4.5mm
10x: 1.85mm
40x: 0.45mm
Features to identify these species under a microscope and if there pro or eukaryotic: Amoeba, Euglena, Paramecium caudatum, Babesia canis
Amoeba: blobby shape, organelles darker (euka)
Euglena: Oval shape, nucleus in centre, small dots around nucleus, multiple of them (Euka)
Paramecium caudatum: oval shaped, pointy ends, large nucleus in centre (Euka)
Babesia canis: clustered little cells, clear dots are blood cells, dark dots (babesia or vacuole) (Euka)
Recall protists from 3 habitats
Freshwater: Amoeba, Euglena, Paramecium caudatum
Marine: Foraminifera, Radiolaria
Hosts: Babesia canis
What are protists
- Evolved from bacteria
- Not fungi, animals or plants
- Uni/Multicelluar, colonial
- Simple organisation, no highly specialised tissues
- Requires moisture
- Paraphyletic group
Differences from prokaryotic and eukaryotic cells
Prokaryotic: single celled, no nucleus/membrane organelles
Eukaryotic: single/mutli celled, DNA in nucleus, has organelles
