Practical 1 Flashcards
(28 cards)
What is this lab equipment? 1
“Centrifuge
- spin PCV tubes, blood samples, to separate serum and plasma”
What is this lab equipment? 2
“Blood tube rocker
- keep blood well mixed “
What is this lab equipment? 3
“Critoseal
- seal PCV tubes “
What is this lab equipment? 4
“Microhematcrit card reader
- read PCV values “
What is this lab equipment? 5
“Microscope
- perform blood smear evaluation “
What is this lab equipment? 6
“Refractometer
- read plasma protein *calibrate daily “
What is this lab equipment? 7
“Capillary tube / hematocrit tubes
- hold a small volume of blood
What is this lab equipment? 8
“Differential counter
- count WBCs”
“Microscope Guidelines:Identify the objective used for each step listed.
A= Coarse focus adjustment knob
B= Fine focus adjustment knob
C= Fine focus adjustment knob”
"A= Coarse focus adjustment knob(Moving stage –focus) B= Fine focus adjustment knob(Finding monolayer) C= Fine focus adjustment knob(Cell identification with oil)"
What are the types of tubes for blood
“A - Green top - Heparin, Yields plasma, for chemistry
B - Red top - no anticoagulant, yields serum, chemistry or electrophoresis
C - Grey/green top - Heparin, yields plasma, for chemistry
D - red top + gel, yields serum, for chemistry ELP,
E - Lavender top, EDTA, whole blood, for hematology, cytology, flow cytometry, coombs test
F - blue top - citrate, plasma, coagulation “
What is the best tube for hematology?
EDTA / lavender
What are the 3 layers of PCV after centrifugation?
Plasma, Buffy(WBCs/Platelets),RBCs
What are the normal PCV measurements?
“Canine 37-55%
Feline 30-45%
Equine 24-64%
Bovine 24-36%”
What are causes of increased/decreased PCV
“Increased - dehydration, Polycythemia
Decreased - anemia, blood loss, excess EDTA”
What are the words used to describe the plasma appearance of a tube?
“Regular - clear, straw
White - Lypemic
Red - Hemolysis
Yellow - Iceteric “
What are the sources of error when preparing PCV tubes?
”- Not enough blood (2/3-3/4 tube)
- spin out/ clay lost
- increased anticoagulant
- specimen not mixed
- too much time elapsed
- centrifuge forced to stop “
What are the 3 different parts of the smear?
Heal, Monolayer/body/critical area, feather
What are the criteria for a good blood smear?
”- Covers 2/3-3/4 slide
- Rainbow effect
- spread across the slide
- no hesitaiton
- rounded
- even distribution
- no waves, streaks, holes
- spread before drop dries “
What are the sources of error when making smears (A-H)
"A - Chipped/rough slide B - Hesitation C - Spreader slide too fast D - too little blood E - Drop not spread out F - Dirt/grease on slide G - Uneven pressure on slide H - Time delay "
What is the name of the stain used for hematology?
Wright’s stain or Romanowsky
What are the staining steps?
"methanol fix (10) buffered eosin (7) buffered thiazine/methylene blue (5) rinse with DW Air dry "
What is the function of Neutrophils
(Seg) phagocytosis
What is the function of Lymphocytes
Innate/Adaptive immunity
What is the function of Eosinophils
Allergic, parasitic rxns, Hypersensitivity
