PRACTICAL PAPER Flashcards

1
Q

independent variable

A

the factor that changes in an investigation

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2
Q

dependent variable

A

the factor that changes as a result.

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3
Q

controlled experiment

A

experiment to ensure independent variable is causing results

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4
Q

standardizing temperature

A

Thermo-statically controlled water bath

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5
Q

standardizing pH

A

pH Buffer solution of known concentration

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6
Q

standardizing light

A

Heat-shielded lamp set at constant

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7
Q

standardizing intensity

A

Distance/power

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8
Q

standardizing wind speed

A

Fan set at constant distance & power

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9
Q

measuring temperature

A

Thermometer

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10
Q

measuring colour

A

Colorimeter

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11
Q

measuring pH

A

pH indicator / pH meter

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12
Q

measuring mass

A

balance

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13
Q

measuring length

A

Microscope with calibrated eyepiece graticule/Ruler.

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14
Q

measuring volume

A

Beaker/Measuring cylinder/Burette/Pipette.

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15
Q

measuring time

A

stopwatch

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16
Q

how to read measuring cylinders accurately

A

Read from bottom of meniscus, perpendicular to scale

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17
Q

accuracy

A

closeness to true value

18
Q

improving accuracy

A

better instruments

19
Q

precision

A

closeness to repeated readings

20
Q

improving precision

A

control all variables

21
Q

reliability

A

confidence in results

22
Q

improving reliability

A

repeat readings and take mean

23
Q

validity

A

agreement between hypothesis and investigation

24
Q

improving validity

A

check relation between key and derived variables

25
systematic errors
result from uncertainties in measurements.
26
random errors
differ across investigation as they arise owing to difficulties in controlling standardised variables and measuring dependent variable.
27
common error sources
- Anomalous readings (owing to inadequate technique/replicates) - Inadequate range and intervals. - Uncontrolled variables.
28
state the actual error in measuring an increase in temperature of 6 degrees celsius using a thermometer
1 degrees celsius you can read reading 0.5 degrees out for one recording and 0.5 degrees out for the other recording so error would be 1 degrees
29
measuring temperature from water in a beaker source of error and how to improve it?
1) energy may be transferred to the surroundings and won't stay in the water - Use insulation or lids
30
observable features of Xerophytes
many air spaces - for buoyancy - and relatively small amount of xylem as water diffusing through epidermis
31
counting bubbles sources of error and how to improve them?
1) Different sizes of bubbles released - Use a gas syringe to measure volume of gas released in total 2) Too many bubbles so not all are counted - Video in slow motion and count from the video so no bubbles are missed out
32
getting enzymes from a fruit or vegetable (like potatoes) source of error and how to improve it?
1) Species of potato have different enzyme catalase | - Use the same potato or same species of potato
33
describe how to standardise the position of the delivery tube in the test-tube of water
Make a mark on the delivery tube to standardise the position of delivery tube
34
judging end points source of error and how to improve it?
1) Difficult to judge when the colour has changed | - Use a colour chart for comparison or use a colorimeter
35
lack of precision or accuracy in measuring instruments
1) Measuring instrument (thermometer, colorimeter) is not properly calibrated (not accurate) - Ensure calibration occurs or use alternate instruments that allow you to calibrate it 2) Using imprecise instruments / methods - Use instruments that have smaller divisions E.g. using a graduated pipette instead of syringe - Take measurements more often
36
when talking about modifications to experiment
- don't forget to mention exactly how much of each sample. etc. like 5 different concentrations or 5 different sized cubes - mention an exact concentration as well
37
using a grid to find area of a specimen - limitations and improvements
- count number of squares that cover more than half of the square improvement : count using a grid with smaller squares
38
drops of milk sources of error + improvement
1) Different sizes of drop of milk | - Use a small syringe or graduated pipette
39
when talking about enzymes ALWAYS mention
- enzyme substrate complexes - higher temp --> higher kinetic energy --> more collisions - denaturing
40
reducing sugar test
- 80 degrees celsius in thermostatically controlled waterbath - Use benedict's solution - reducing sugar present - turns from blue to brick red
41
non-reducing sugar test
sucrose is a non-reducing sugar made of glucose and fructose which are reducing sugars - add HCL and heat gently to break down non-reducing sugar into reducing sugars (glycosidic bonds) - neutralise the sample with sodium hydrogen carbonate solution - add benedict's reagent (80 degrees in water bath) - observe colour change
42
protein test
- add sodium hydroxide and copper sulfate solution to sample (biuret solution) - if protein present solution turns from blue to purple