Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Micro Practical 2 > Practical2 > Flashcards

Practical2 Flashcards

(66 cards)

Start Studying
1
Q

Amylase Test Test for?

A

? amylase enzyme which hydrolysis starch into glucose subunits

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Amylase Test Medium

A

Starch agar appears clear transparent iodine regents to visualize

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Amylase Test + vs -

A

+ clear - Brown

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Lipase Tests For?

A

lipase which hydrolysis tributyrin into glycerol and three fatty acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Lipase Test Medium

A

Tributyin agar(cloudy with water)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Lipase + or -

A

clear + and cloudy -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Gelatinase Test Tests For?

A

gelantinase which hydrolyses gelatin into amino acid subunits

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Gelatinase Test

A

Medium gelatin agar(cloudy with water)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Gelatinase + or -

A

clear + and cloudy -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Catalase Test Test for

A

catalase which breaks down hydrogen peroxide into h20 and o2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Catalase Test Medium

A

hydrogen peroxide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Catalase + or -

A

bubbles + and no bubbles -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Oxidase Test For?

A

Cytochrome c oxidase which is the last component of the electron transport chain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Oxidase Test Medium

A

BBL Dry Slide(oxidase slide)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Oxidase Test + or -

A

Purple within 20sec + and no color change or light gray is -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Nitrate Reduction Tests for?

A

N2 Nitrate reductase(ability to reduce nitrate to n2 gas) also test for denitritrication

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Nitrate Reduction Medium

A

Nitrate Broth test tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Nitrate Reduction Test + or -

A

Bubble + add reagents sulfuric acid and Tromsdorff if turn purple brown then + and - no bubble and yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Fermentation of Carbohydrates Test for which 3 sugars

A

glucose lactose and sucrose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Fermentation of Carbohydrates Medium

A

Fermentation broth with glucose; lactose and sucrose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Fermentation of Carbohydrates test results (A; AG; NC; K)

A

A-Acid(yellow) AG-Acid Gas(yellow and gas in tube) NC- no change or neutral green K- Alkaline(blue)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Methyl Red Voges prokauer(MR-VP) Test for?

A

Two different glucose fermentations pathways MR- mixed acid pathway VP for 2;3 butanediol pathway

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

MR-VP Medium

A

MR-VP broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

MR-VP Test Results

A

MR = Red + and yellow - VP = yellow dark +(20m) and yellow copper -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
SIM TEST Test For
Hydrogen Sulfide H2S/Indole /Motility
26
SIM Medium
SIM Medium
27
Hydrogen Sulfide H2S Test(S) and results Test for
Cysteine Desulfurase Enzyme Results black + and yellow -
28
Indole Test For?
Tryptophanase enzyme that produces pyruvate and indole +NH3
29
Indole Test Medium and Results
SIM medium paper strips pink is +
30
Motiltiy Test Medium and Results
SIM medium +fuzzy and -not fuzzy
31
Phenylalanine Deminase Test For and Medium
Phenylalanine deminase on a Phenylalanine slant agar
32
Phenylalanine Deminase + and -
+ green medium - is clear
33
Urease Test Test for and Medium?
Urease enzemye that produces Nh3 ammonia and Urea Agar (SLANT) with Ph indicator
34
Urease Test + and -
+fuchsia(pink) and - yellow
35
Citrate Test
Citrate permease enzyme that can utilize citrate as their sole carbon source
36
Citrate Test Medium and Results
Simmon's citrate agar (Ph indicator) Alkaline= (+)Blue and Green (-)
37
5 leukocytes White blood cells
Neutrophils ; Lymphocytes; Monocytes; Eosinophils; Basophils (Never Let monkeys Eat Bananas)
38
Antibiotic Sensitivity how to interpret zones of inhibition as being susceptible; intermediate or resistant.
R-Resistant equal to or less than that of the inner circle I- intermediate Zone greater than R but less than that of the outer circle S-Sensitive - Son with a diameter equal to or greater than outer circle
39
pGLO genes are present on the engineered plasmid 3
1 bla gene(amplicillin resistance Marker gene) 2 GFP gene Green Fluorescent Protein Gene3 AraC geen/pbad promoter arabinose production
40
pGLO how do u introduced the plasmid into the host E. coli.
Heat shock - 42hot water 50 sec then add in LB nutrient broth 10 min
41
Epidemiology
studying the location and timing of diseases and how they spread
42
epidemic
incidence of a disease in a population at greater than expected levels
43
Stimulated Epidemic Identify the bacteria used in this experiment
Micrococcus Luteus
44
Cystitis
lower tract infection leads to bladder infection
45
Pyelonephritis
Infection in the upper tract leads to kidney infection
46
Dysuria
urgent need to urinate(painful;difficult)
47
pyuria
White blood Cells
48
hematuria
Red blood Cells
49
Describe the two sources of urinary tract infections and the risk factors that promote development of disease.
1)Own Flora 2) spread of systemic infection UTI Risk Factors: Women Catheter; neurological compromise
50
Urine Culture Medium 2
1)Sheep Blood Agar promotes Gram+ and Gram - 2) MacConkey agar - selective and differential medium (selects fgram neg bacteria and it differentiates lactose fermenters with Ph indicator
51
Urine Culture Test Results
lactose fermenters(pink) vs (White) non-lactose fermenters
52
Neisseria Gonorrhoeae 1)Eetiologic agent; 2) symptoms 3) the mode(s) of transmission.
1)etiologic agent Gram- dipococcus 2)Symptoms Pus discharge from urethra/Subclinical 3) Mode of Tran. STI(sexually transmitted infection or Mother baby
53
Taenia solium 1)Eetiologic agent; 2) symptoms 3) Modes transmission.
Taeniasis/cycticercosis 1)Eetiologic agent - Cestode Tapeworm 2) symptoms cystis 3) the mode(s) of transmission Fecal Oral Transmition
54
Trypanosoma Brucie Gambiense 1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission.
African trypansomiasis/Sleeping sickness 1)Eetiologic agent - protozoan parasite2) symptoms sweling of lyph nodes joint pain itching 3) the mode(s) of transmission - Tsete Fly
55
Ascaris Lumbricoides 1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission
Acriasis 1)Eetiologic agent - Worm 2) symptoms -worms exciting from orifeces asymptomatic 3) the mode(s) of transmission Oral Fecal
56
Epstein - Barr Virus
1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission - Infectious monocucleosis (mono)1)Eetiologic agent - herpes Virus 2) symptoms fatigue body malaise 3) the mode(s) of transmission kissing exchanging saliva
57
Bacillus anthracis - 1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission
inhalational anthrax 1)Eetiologic agent gram + spores 2) symptoms vomiting of blood systemic hemorrhagic 3) the mode(s) of transmission inhalation
58
Plasmodium Falciparum -1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission-
Cerebral malaria 1)Eetiologic agent Parasitc protozoan 2) symptoms Flu like headaches shivering ; heolytic anemia 3) the mode(s) of transmission Anopheles mosquito
59
Schistosoma mansoni
1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission - Chistosomiasis(flatworm) 1)Eetiologic agent Parasite tremotode 2) symptoms rash itching damage to vital organs 3) the mode(s) of transmission snails/freshwater
60
Canidia albicans 1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission
1)Eetiologic agent Candidiasis Fungus 2) symptoms- Vaginal extreme itching white discharge3) the mode(s) of transmission Sexual intercourse
61
Giardia Lamblia 1)Eetiologic agent 2) symptoms 3) the mode(s) of transmission
giardiasis Interindids 2) symptoms diarrha abdominal cramps Vaginal 3) the mode(s) of transmission h20 contamination
62
Know what causes Ebola and where this epidemic occurred
Ebola virus in Zahier kismet
63
Describe the symptoms of Ebola
bleed to death
64
Throat Culture medium used and what you are testing for?
Sheeps Blood Agar(streak for isolation) (differential helps categorize hemolysic patterns)
65
Throat Culture plates; differentiate alpha; beta and gamma hemolysis?-
Alpha - greenish pigment (normal flora) Beta - complete lysis of RBC clear zone (Pathogen) Gamma no clearing (normal flora)
66
Which type of hemolysis is considered pathogenic; and give examples of possible pathogens.
Staphlyococcus aureus and streptococcus pyogenes

Micro Practical 2 (3 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions