practicals Flashcards
1
Q
State the Beer-Lambert law
A
the absorbance of a solution is proportional to the concentration of the absorbing solute, and to the distance travelled by the light through the sample
A = E x c x l
2
Q
What is Vmax?
A
Maximum rate of reaction
The rate of reaction when the enzyme is saturated with substrate.
3
Q
What is Km?
A
the concentration of substrate which permits the enzyme to achieve half Vmax.
An enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.”
