Pre-Matriculation

Question 1

Are the acidic AA positively or negatively charged?

Answer 1

negatively

Question 2

How do competitive inhibitors affect Km and Vmax?

Answer 2

no change in Vmax, decrease Km

Question 3

How do noncompetitive inhibitors affect Km and Vmax?

Answer 3

decrease Vmax, no change on Km

Question 4

How do uncompetitive inhibitors affect Km and Vmax?

Answer 4

decrease Vmax, and decrease Km

Question 5

Name the Acidic Amino Acids

Answer 5

Aspartate and Glutamate

Question 6

Name the Aromatic Amino Acids

Answer 6

Phenylalanine, Tyrosine, Tryptophan

Question 7

Name the Basic Amino Acids (positively charged)

Answer 7

Arginine, Lysine, and Histidine

Question 8

Name the Non-polar aliphatic Amino Acids

Answer 8

Glycine, Alanine, Valine, Leucine, Isoleucine, and Proline

Question 9

Name the Polar uncharged amino acids

Answer 9

Asparagine, Glutamine, Serine, Threonine

Question 10

What are domains?

Answer 10

contiguous seq of aa that fold independently of the rest of the protein (tertiary structure of proteins)

Question 11

what are folds?

Answer 11

3D structures that have the same arrangement of motifs and domains but do not necessarily have the same primary sequence (tertiary structure)

Question 12

what does slope of Lineweaver-Burke plot mean?

Answer 12

Km/VMax

Question 13

What does the x-interept of Lineweaver-Burke plot indicate? Y-intercept?

Answer 13

-1/Km; 1/Vmax

Question 14

what is the hill coefficient in cooperativity? (n)

Answer 14

A coefficient that defines amount of cooperativity displayed. N<1 negative cooperativity (more hyperbolic curve); N>1 more sigmoidal curve because positive cooperativity