pre quiz and week 1 Flashcards
bond energy when breaking bonds
energy must be added
bond energy when making bonds
energy is released
protonation when pH < pKa
protonated
protonation when pH > pKa
deprotonated
what is H bond donor
group providing an H bonded to an N, O, or F
what is an H bond acceptor
group accepting H – N, O, or F with a lone pair; does not require an H
where do H bonds occur in secondary structure
between amino H and O of carbonyl
how is an alpha helix arranged
tightly, so R groups point out and H bonds occur between layers and there is no free space inside helix
what is the henderson-hasselbalch equation?
pH = pKa + log[A-]/[HA]
what is a buffer
solution which can resist strong change in pH
where is buffering region
+/- 1 pH unit from pKA
where is equivalence point
steep part of titration curve
why are buffers important
changes in pH can affect protonation state of amino acids which can alter protein structure and therefore function
what is a steady state
input = output
changing concentrations, but no net changes
ratios are different than at equilibrium
open system
what is equilibrium
closed system where rate forward = rate backwards and concentrations of components do not change
