Preliminary ID And Properties

Question 1

Staphylococcus aureus

2

Answer 1

Coagulase +

Ferments mannitol

Question 2

Staphylococcus epidermidis

3

Answer 2

Coagulase -
Non hemolytic
Does NOT ferment mannitol

Question 3

Staphylococcus saprophyticus

2

Answer 3

Coagulase -

Novobiocin resistant

Question 4

Streptococcus pyogenes

5

Answer 4

Chain of cocci
Beta hemolysis
Group A
PYR +
Bacitracin sensitive

Question 5

Streptococcus agalactiae

5

Answer 5

Chain of cocci
Beta hemolysis 
Group B
Bacitracin resistant 
CAMP +

Question 6

Streptococcus pneumoniae

4

Answer 6

lancet shaped diplococci
Alpha hemolytic
Bile sensitive
Optochin sensitive

Question 7

Enterococcus

4

Answer 7

Pairs/short chains of cocci
Gamma hemolytic
Bile resistant
Antibiotic resistant

Question 8

Bacillus anthracis

5

Answer 8

Non motile
Non hemolytic
String of pearls
Unique capsule
Medusa head colonies

Question 9

Bacillus cereus

3

Answer 9

Motile
Hemolytic
Emetic and diarrheal toxins

Question 10

Clostridium perfringens

5

Answer 10

Box shaped
Anaerobic
Rapid growth
Many toxins 
Nagler reaction

Question 11

Corynebacterium diphtheriae

2

Answer 11

Pleomorphic rod (Chinese letters)
dark pharyngeal pseudomembrane

Question 12

Listeria monocytogenes

3

Answer 12

Catalase +
Tumbling motility at room temp
Umbrella motility

Question 13

Actinomyces israelii

3

Answer 13

Anaerobic
Molar tooth colonies
Sulfur granules

Question 14

Nocardia spp

3

Answer 14

Weakly Acid fast
Slow growth
Fuzzy colonies (aerial hyphae)

Question 15

Mycobacterium tuberculosis

4

Answer 15

Strongly acid fast
Slow growth
non pigmented colonies
Mantoux and Quantiferon tests

Question 16

Neisseria meningitidis

4

Answer 16

Diplococci
Fastidious
Can grow on chocolate agar
Gram stain blood/CSF before antibiotics

Question 17

Neisseria gonorrhoeae

4

Answer 17

Diplococci
In PMN cells in urethritis specimen
Fastidious
Grows on Thayer Martin agar

Question 18

Moraxella catarrhalis

2

Answer 18

Diplococci

Grows on chocolate and blood agar

Question 19

Acinetobacter spp

3

Answer 19

Very small rods in pairs
Oxidase -
Grows on MacConkey

Question 20

Enterobacteriaceae

2

Answer 20

Oxidase -

Grows on MacConkey

Question 21

Enterobacteriaceae
Lactose fermenters
2

Answer 21

E. coli

Klebsiella

Question 22

Enterobacteriaceae
Lactose non-fermenters
4

Answer 22

Salmonella
Shigella
Yersinia
Proteus

Plus pseudomonas (which is not enterobacteriaceae)

Question 23

Enterobacteriaceae
Citrate positive
1

Answer 23

Klebsiella

Question 24

Enterobacteriaceae
Urease positive
2

Answer 24

Klebsiella

Proteus

Question 25

Enterobacteriaceae H2S producer 1

Answer 25

Salmonella

Question 26

Bacteroides, Fusobacterium | 2

Answer 26

Weakly staining | Anaerobic

Question 27

Pseudomonas aeruginosa | 5

Answer 27

``` Oxidase + Green Grape like smell Growth on MacConkey Lactose non fermenter ```

Question 28

Vibrio cholerae

Answer 28

Ferments sucrose | Thiosulfate citrate bile salt sucrose (TCBS) agar-yellow colonies

Question 29

Helicobacter pylori | Test

Answer 29

Urea breath test

Question 30

Campylobacter spp | 2

Answer 30

Thin, S shaped rod Growth on media with antibiotics at 42 degrees C

Question 31

Haemophilus spp | 3

Answer 31

Small coccobacilli X and V factor Satellite phenomenon (growth near Staph aureus on blood agar)

Question 32

Legionella spp | 3

Answer 32

Weak staining Buffered charcoal yeast extract agar (BCYE) Needs cysteine to grow

Question 33

Bordetella pertussis | 3

Answer 33

Fastidious Regan Lowe charcoal agar Use Dacron swabs (no cotton)

Question 34

``` Yersinia pestis Looks like (2) ```

Answer 34

Safety pin appearance | Fried egg colonies

Question 35

Brucella spp | 2

Answer 35

Very small coccobacilli | Slow growth

Question 36

Francisella spp | 3

Answer 36

Very small coccobacilli Slow growth Biohazard

Question 37

Treponema pallidum | Testing

Answer 37

If positive RPR and VDRL, confirm by FTA-ABS or MHA-TP

Question 38

Borrelia burgdorferi | Dx by__

Answer 38

serology

Question 39

Borrelia hermsii | Serology?

Answer 39

Serology is useless

Question 40

Leptospira spp 2 Shape Dx

Answer 40

Hook shaped | Dx by serology

Question 41

Rickettsia spp 3 Tests

Answer 41

Poorly staining Fluorescent antibody test Weil-Felix test

Question 42

Erlichia and Anaplasma 3 Tests

Answer 42

Poorly staining PCR serology

Question 43

Coxiella burnetti 2 Tests

Answer 43

PCR | Serology

Question 44

Chlamydia species | 3

Answer 44

No cell wall NAAT test Inclusion bodies

Question 45

Mycoplasma species 5

Answer 45

``` No cell wall PCR Eaton's agar Mulberry shaped (M. pneumoniae) Fried egg shape (all others) ```

Question 46

All strep are catalase __

Answer 46

Negative

Question 47

All staph are catalase __

Answer 47

Positive

Question 48

All enterococcus and enterobacteriaceae are catalase ___ Enterobacteriaceae are oxidase ___

Answer 48

Negative Negative

Question 49

Urease positive

Answer 49

PUNCH K ``` Proteus Ureaplasma Nocardia Corynebacterium Helicobacter Klebsiella ```

Question 50

Oxidase positive

Answer 50

PVNCH ``` Pseudomonas Vibrio Neisseria Campylobacter Helicobacter ```

Question 51

Urease negative | Key bacteria

Answer 51

Salmonella | Shigella

Question 52

Oxidase negative | Key bacteria

Answer 52

Salmonella Shigella Proteus

Question 53

H2S producer

Answer 53

Salmonella