PRIMARY SETUP MEDIA RECOMMENDED FOR RECOVERY OF ANAEROBES

Question 1

Supports growth of almost all obligate
and facultative anaerobes, best for
anaerobic gram-positive cocci

Answer 1

Anaerobic blood agar (CDC)

Question 2

Supports growth of bile-tolerant
Bacteroides spp., some strains of
Fusobacterium mortiferum, Klebsiella
pneumoniae, enterococci, and yeasts
may grow to a limited extent

Answer 2

Brucella blood agar

Question 3

Supports growth of almost all obligate
and facultative anaerobes, best for
gram-negative bacteria

Answer 3

Kanamycin–vancomycin–laked
blood agar

Question 4

Supports growth of Bacteroides and
Prevotella spp.; yeasts and
kanamycin-resistant, facultative, gramnegative bacilli will also grow

Answer 4

Phenylethyl agar

Question 5

Supports growth of almost all obligate
anaerobes (gram-positive and gramnegative) and gram-positive,
facultative anaerobes

Answer 5

Colistin–nalidixic acid blood agar
plate

Question 6

Supports growth of almost all types of
bacteria; in thioglycollate broth,
obligate aerobes and microaerophiles
grow near the top, obligate anaerobes
at the bottom, and facultative
anaerobes throughout the broth

Answer 6

Anaerobic broth (e.g., thioglycollate
and chopped or cooked meat)