Principles Of Microbiology Flashcards

1
Q

What are the four classes of cellular molecules?

A

Proteins, nucleic acids, lipids and polysaccharides.

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2
Q

Six characteristics of living organisms.

A

Compartmentalization and metabolism, growth and reproduction, differentiation, communication, movement and evolution.

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3
Q

Compare machine and coding functions of microbial cells.

A

Machines: metabolism and enzymes accelerating it.
Coding devices: store and process DNA.
They dependent on each other and have to be highly coordinated in order to provide growth and reproduction.

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4
Q

What a microbial habitat?

A

It’s the environment in which the cell population lives.

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5
Q

What differs microbial community from microbial population?

A

Population: a group of cells originated from the same cell.
Community: populations interacting.

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6
Q

How can microrganisms change the characteristics of their habitats?

A

They remove and excrete nutrients to their habitats. They are therefore open systems.

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7
Q

Were the earliest life forms cellular?

A

No, there were small molecules of RNA able to replicate themselves.

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8
Q

Why is it that cellular life is the only form of life we see on Earth today?

A

They hold the fundamental characteristics of life so they were selected.

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9
Q

How old is Earth and when did cellular life forms first appear?

A

It’s 4.6 billion years old and the first cellular life (methanogens & other anaerobic organisms) is 3.8 billions years old.

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10
Q

How can we use science to reconstruct the sequence of organisms that appeared of Earth?

A

Using biomarkers that can be identified in ancient rocks.

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11
Q

Where are most microbial cells located on Earth?

A

Undergroung in oceans and terrestrial subsurfaces.

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12
Q

In what ways are microorganisms important in the food and agricultural industries?

A

Fermentation, food preservation, Nitrogen metabolism (nodules in legumes’ roots) and rumen.

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13
Q

List two fuels that are made by microorganisms.

A

Methane and ethanol.

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14
Q

What is biotechnology?

A

It’s the genetic modification of microorganisms in order to enable them to produce commercial items such as insulin and other hormones.

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15
Q

What major discovery emerged from Cohn’s study of hear resistance in microorganisms?

A

Bacteria that form endospores.

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16
Q

Define term sterile.

A

Sterile is a medium where there are no microorganisms.

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17
Q

How did Pasteur’s swan-neck flask experiment show that the concept of spontaneous generation was invalid?

A

The fresh air without the microorganisms was unable to create life, which appeared only when the sterile liquid was in touch with microorganisms.

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18
Q

How do Koch’s postulates prove cause and effect for the culture of microorganisms?

A

By inoculating microorganisms present in sick animals into health animals.

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19
Q

What advantages do solid media offer for the culture of microorganisms?

A

They enable colonies to grow and make easy to identify then.

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20
Q

What is a pure culture?

A

It is a culture originated from one single cell.

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21
Q

What is the enrichment culture technique?

A

It’s a selection of microorganisms by manipulating nutrient and incubation conditions.

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22
Q

What’s chemolithotrophy?

A

It’s the oxidation of inorganic compounds such as ammonia and CO2 linked to energy conservation. Therefore some microorganisms can produce ATP from them.

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23
Q

How might you distinguish a phototrophic microorganism from a chemotrophic one by simply looking at it under a microscope?

A

The photrothopic ones are colourful because of their pigments.

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24
Q

What are extremophiles?

A

Microorganisms that require extreme conditions to survive such as high temperatures and salty environments.

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25
Q

What is the largest phylum of Bacteria?

A

Proteobacteria.

26
Q

Why can it be said that the cyanobacteria prepared Earth for the evolution of higher life forms?

A

Because they oxigenated the Earth.

27
Q

What is physiologically unique about Deinococcus?

A

Their resistance to radiation.

28
Q

What is unusual about Halobacterium?

A

They can produce ATP from light in a distinct way than other phototrophs.

29
Q

What group of Archea is responsible for producing the natural gas we use as a fuel? Chemically, what is natural gas?

A

Methanogens. Methane.

30
Q

How can we know the microbial diversity of a natural habitat without first isolating and growing the organisms it contains?

A

By rRNA sequences.

31
Q

List two ways algae differ from cyanobacteria.

A

They have chloroplasts and they can inhabit soil.

32
Q

List two ways algae differ from protozoa.

A

They have cell wall and they are phototrophs.

33
Q

How do each of the components of a lichen benefit each other?

A

Algae or cyanobacteria provide nutrients and fungi provide protection.

34
Q

Why have rocks dating to the origin of Earth not been found?

A

Because the materials were condensed due to energy released by nuclear fusion.

35
Q

What do crystals of the mineral zircon tell us about when the first oceans may have formed?

A

They have traces of oxygen.

36
Q

What does the possible presence of liquid water on Earth 4.4 to 4.3 billion years ago suggest about the origin of life?

A

The conditions were compatible with life already.

37
Q

What roles did the mounds of mineral-rich materials warm hydrothermal springs play in the origin of life?

A

They provided stable conditions for life.

38
Q

What important cell structure was necessary for life to proceed from a possible RNA world to cellular life?

A

Lipid membranes.

39
Q

How could cells have obtained energy from Fe + H2S?

A

Reducing it which is an exergonic process.

40
Q

Why is the advent of cyanobacteria considered a critical step in evolution?

A

Because the oxygenated the Earth.

41
Q

In what form is iron present in banded iron formations?

A

Ferric iron.

42
Q

What role did ozone play in biological evolution, and how did cyanobacteria make the production of ozone possible?

A

It permitted life in sunlight exposed areas. Cyanobacteria produces oxygen, that is converted in ozone when exposed to UV radiation.

43
Q

What evidence supports the idea that the eukaryotic mitochondrion and chloroplast were once free-living members of the domain Bacteria?

A

Morphologic, genetic and phylogenic similarities.

44
Q

In what ways are modern eukaryotes a combination of attributes of Bacteria and Archaea?

A

They have the type of lipids found in Bacteria as well their energy metabolism mechanisms. They transcription and translation apparatus is similar to Archaea’s.

45
Q

According to the Darwinian view of life, all organisms are related by…

A

An ancestor.

46
Q

What class of mutation is most common?

A

Neutral.

47
Q

Why are SSU rRNA genes suitable for phylogenetic analysis?

A
  • universally distributed
  • functionally constant;
  • sufficiently conserved;
  • adequate length.
48
Q

What information does the RDP-II provide?

A

rRNA gene sequences.

49
Q

What value do molecular clocks have in phylogenetic analysis?

A

It gives an approximated time between mutations.

50
Q

How are DNA sequences obtained for phylogenetic analysis?

A

PCR.

51
Q

What does a phylogenetic tree depict?

A

The order of descent and the ancestry of the nodes.

52
Q

Why is sequence alignment critical to phylogenetic analysis?

A

Because it defines how closely related are the species.

53
Q

What are signature sequences?

A

Exclusive rRNA sequences within a domain, genus or single species.

54
Q

How can oligonucleotide probes be made visible under the microscope? What is this technology called?

A

By attaching a fluorescent dye to it (FISH).

55
Q

What kind of questions can be addressed using a microbial community analysis?

A

What species are in that environment.

56
Q

How is ribotyping able to distinguish between different bacteria?

A

By different cuts in DNA.

57
Q

What is FAME analysis?

A

Analysis of fatty acids proportions in cell membrane.

58
Q

What are some of the drawbacks of FAME analysis?

A

It requires rigid standardization and specific growth conditions.

59
Q

How do AFLP and MLST differ from ribotyping?

A

They focus in variations in DNA sequence throughout the genome..

60
Q

What limits the value of 16S rRNA gene or any gene in taxonomy?

A

Too conserved.

61
Q

What is the meant by the term monophyletic?

A

A group descended from one ancestor.

62
Q

What’s an ecotype?

A

A population of cells that compete for the same key nutrients.