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Procedures Flashcards

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1
Q

ALA DISK

A 
For Haemophilus
Porphobilinogens and porphyrins
X-independent strains
Delta-amino levulenic acid
\+ H. Paraflu and H. Parahaem
- H. Flu
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2
Q

Bacitracin

A

Taxo “A”
S-Group A
R-Group B

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3
Q

Beta-lactamase Disk

A

N.G.
Moraxella catarrhalis
H. Flu

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4
Q

Bile Esculin

A

Esculin and ferric citrate and 4% oxbile

+Enterococcus Faecalis
-Streptococcus agalactiae

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5
Q

Bile solubility

A

Desoxycholate reagent
+Streptococcus pneumoniae
-Streptococcus salivarius

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6
Q

Catalase

A

H2O2 —> H2O + O2
+Staphylococcus aureus
-Streptococcus pyogenes

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7
Q

CAMP

A

Uses Staphylococcus aureus
+Streptococcus agalactiae
-Streptococcus pyogenes
Shovel shape

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8
Q

Catarrhalis

A

Butyrate esterase
Bromo-chloro-indolyl butyrate
+Moraxella catarrhalis (blue-green)
-Neisseria lactamica

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9
Q

Simmons Citrate Agar

A

GN
Green to blue+
+klebsiella pneumoniae
-Escherichia coli

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10
Q

Coagulase

A

Lyophilized rabbit plasma
Rapid slide-bound coagulase
Tube-free coagulase

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11
Q

CTA

Cystine trypticase Agar

A 
N.G. = Glucose
N.M. = Maltose
N.L. = Maltose and Lactose
N.S. = Maltose and Sucrose
Moraxella = -
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12
Q

D Test

A

Clindamycin
+Clindamycin resistance
-Inducible clindamycin resistance
-Constitutive clindamycin resistance

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13
Q

Decarboxylase Broth (Moeller) LAO

A

Lysine
Arginine
Ornithine
Bromcresol purple and cresol red

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14
Q

DNAse Agar

A

Toluidine Blue
+Serratia marcescens and Staphylococcus aureus
-Coag Neg Staph
+Pink to Red Zone or Clearing with Methyl Green

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15
Q

E-Test

A

Gradient Diffusion Method

GN aerobes and Staphylococci, H. flu, and Strep. pneimoniae

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16
Q

Germ Tube

A

Candida albicans

Beginning of true hyphae

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17
Q

Group B Strep Detect and Carrot Broth

A

+Step. agalactiae

Entero. faecalis

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18
Q

Haemophilus Identification Agar (HID I)

A
  1. hemin - X factor
  2. NAD - V factor
  3. XV factors
  4. NAD + Horse blood
    I, II, III, IV - H. aphrophilus
    III, IV - H. flu
    II, III, IV w/beta - H. parahaem
    II, III, IV - H. paraflu
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19
Q

Hippurate Hydrolysis Rapid

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A

Sodium hippurate, Hydindantin, ammonia, nihydrin

+Purple = Group B Strep, Gardnerella vaginalis, Campylobacter jejuni

20
Q

Hodge Test

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A

Trypticase Soy, Ertapenem disk
+ Klebsiella pneumoniae ATCC BAA-1705
-1706

21
Q

Indole Test

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A

Benxyl pyrrol
Tryptophan
+E. Coli (Red)
-Klebsiela pneumoniae

22
Q

Kligler Iron Agar (KIA)

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A

Phenol Red
AK/A - Morganella morganii
AK/AK - E. Coli
AK/A - Salmonella sp. (H2S+)

23
Q

Kirby-Bauer Susceptibility

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A

Susceptibility
E. coli
Klebsiella pneumonia
Staph aureus

24
Q

LAP Test

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A 
Leucine aminopeptidase
DIsk
Leucine and Beta naphthylamide
Turns pink/orange
\+ Entero. faecalis
Aero. viridans
25
Lysine Iron Agar (LIA)
Bromcresol Purple, Ferric ammonium citrate and thiosulfate. K/K - lysine decarboxylation - Klebsiella K/A - Only glucose fermentation - Salmonella R/A - Lysine deamination - Proteus/Providencia K/A H2S+ -Glucose and H2S fermentation - Citrobacter
26
LIM and Carrot Broth
Colistin and nalidixic + orange - no change + Beta hemolytic. Catalase Negative GPC
27
Mannitol Salt Agar
7.5 Sodium chloride Phenol Red + Staph aureus (yellow) - CNS
28
MR-VP
Methyl Red 5% alpha-naphthol then potassium hydroxide +- E. Coli -+ Entero aerogenes
29
Microdase Disk
DMSO TMDP into cell + blue = Micrococcus - NC = Staph spp.
30
Motility Indole Ornithine (MIO)
Tryptophanase and Bromcresol purple +++ E. coli --- Klebsiella pneumoniae
31
Motility Agar
+ E. coli | - Klebsiella pneumoniae
32
MRSA and Vancomycin
``` Methicillin resistant Staph Aureus with Oxicillin Sometimes Chromogenic Agar for Both VRE Vancomycin Resistant Enterococci Growth is resistance NG is sustibility ```
33
6.5% NaCl
+ Enter. Spp | - Group D Strep
34
Nitrate Test
Nitrate to Nitrite Sulfanilic acid and N,N-dimethyl-alpha-naphthylamine + E. coli - Acinetobacter baumannii
35
Novobiocin Disk
S - Staph epi | R - Staph saprophyticus
36
Optochin Disk
Eythlhydrocupreine hydrochloride 5ug + Step pneumoniae - Virdans strep and Entero. faecalis
37
Oxidase
Indophenol oxidase Pink/Purple + + Psudomonas aeruginosa - E. coli
38
Oxidative Fermentation
``` Bromthymol blue makes green to yellow Open tube/Oil Oxidative A/NC Fermentative A/A Neither NC/NC ```
39
Phenylalanine Agar (PDA)
Phenylpyruvic acid | + is green
40
PYR Test
L-pyrrolindonyl-Beta-nephthylamide + Entero faecalis or Group A Strep - Group B Strep
41
Rapid NH
Neisseria Unknowns
42
Staphyaurex Latex
Protein A | + Staph aureus
43
Streptex Acid Extraction
Agglutination + Lancefield Streptococci
44
Superoxol Catalase
N. gonorrhoeae with 30% Peroxide
45
Synergy Quad Plates
I: Control Pink II: Vancomycin 6mg III: Gentamicin 500mg IV: Streptomycin 2000mg
46
Urea Agar
Phenol red + Proteus mirabilis = E. coli
47
X&V Factor
H flu XY | H aphrophilus X or Y

Microbiology (11 decks)

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