Prokaryotic DNA gene expression

Question 1

upstream

Answer 1

3’ to 5’

Question 2

downstream

Answer 2

5’ to 3’

Question 3

where is the promoter in relation to the gene of interest?

Answer 3

slightly upstream

Question 4

What are the 3 steps of transcription?

Answer 4

initiation
elongation
termination

Question 5

holoenzyme

Answer 5

complex of catalytic core and sigma factor
example: RNA polymerase

Question 6

sigma factor

Answer 6

component of a holoenzyme and acts to positiion it at the promoter region of the template strand.

Question 7

Transcription: Initiation

Question 8

Transcription: elongation

Question 9

what are the 2 types of termination and how do they differ?

Answer 9

intrinsic termination (rho-independent)
rho-dependent termination

Question 10

intrinsic termination

Question 11

rho-dependent termination

Question 12

what is the initiation sequence called in DNA transcription?

Answer 12

the promoter

Question 13

what are transcriptional regulators?

Question 14

describe the difference between postiive and negative regulation of transcription INITIATION:

Question 15

what is the operon

Question 16

polycistronic

Question 17

what are the different parts of the lac operon and what are their functions?

Question 18

Lac I

Question 19

Lac Z

Question 20

Lac Y

Question 21

Lac A

Question 22

permease – on the _ gene

Question 23

repressor protein

Question 24

transacetylase

Question 25

what does the operator do?

Question 26

shine-delgarno sequence

Question 27

what are the 3 sites on a ribosome?

Answer 27

A site = aminoacyl site P site = peptidyl site E site = Exit site