prokaryotic transcription

Question 1

feature that is similar in both transcription and replication

Answer 1

RNA synthesis require DNA topoisomerase action

Question 2

synthesis of mRNA on DNA template is

Answer 2

unidirection

Question 3

mechanism that checks to make sure that the right monomer is incorporated into the polymer

Answer 3

RNA synthesis

Question 4

enzyme that does not require a primer

Answer 4

DNA dependent RNA polymerase

Question 5

E.coli polymerase have

Answer 5

holoenzyme include sigma factor, require Mg2+ and Zn2+

Question 6

DNA binding protein that initiates the transcription of bacterial genes is called

Answer 6

sigma factor

Question 7

sigma subunit of RNA polymerase

Answer 7

specifies the site for transcription

Question 8

binding of sigma factor depends on the

Answer 8

recognition of the length and spacing of promotor consensus sequence

Question 9

role of the promotor region of a gene

Answer 9

site where RNA polymerase binds to DNA

Question 10

pribnow box is centered at

Answer 10

-10 position

Question 11

mode of action of rifampicin in E.coli is through

Answer 11

initiation of transcription and binding of RNA polymerase to DNA template

Question 12

TATA box plays a key role in ascending active transcription

Answer 12

binding with TATA box binding protein

Question 13

transcription factors bind to TATA box

Answer 13

TFIID