protein purifiaction Flashcards
1
Q
cloning
A
start and end gene on opposite strans
chose RE and into pet28 to cut the plasmid and ligase
2
Q
protien production controlled by
A
T7 Lac operator
3
Q
chromotography
A
based on size, charge and chromotography
4
Q
Affinity chromotograpghy
A
- bind- low imidozole
- wash
- elute - high imodzole
5
Q
SDS page
A
visualze protiens and denature to add charge makes in (-)
