protein synthesis Flashcards

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1
Q

how are genes expressed?

A

proteins

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2
Q

what is a gene

A

a section of a dna which codes for a protein .

made up of nucleotides

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3
Q

what does RNA do in the process of protein synthesis?

A

transports messages from DNA to ribosomes.

helps produce proteins (t-RNA)

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4
Q

what happens if a gene is faulty?

A

regular protein is not produced.

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5
Q

functions of proteins

A
structural
regulatory
transport
defense
catalytic
contractile
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6
Q

what is each code consisting of?

A

codon - three nucleotide

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7
Q

characteristics of a dna triplet code are?

A

redundant/degenerate(more than one can code for a protein)
unambiguous(code for only one aminoacid)
non overlapping(read in groups of three)
universal

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8
Q

the two stages of protein synthesis

A
  1. transciption

2. translation

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9
Q

what is transcription ?

A

base sequences in DNA are copied onto m-rna as codons

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10
Q

what is translation?

A

codons of m-rna are used to produce proteins

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11
Q

steps of the process of transcription.

A
  1. Helicase (an enzyme) attaches to a region of DNA in the template strand.
  2. the exposed base sequences are bound to complementary nitrogenous bases to form pre-mRNA by the movement of RNA polymerase along the template dna strand.
  3. pre-mrna is modified.
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12
Q

what is post-transcription modification?

A

when pre mrna is modified to mrna.

  1. introns are cut out.
  2. extrons are bonded to form a shorter mrna .
  3. mrna is chemicaaly capped (methyl cap)
  4. poly-A-tail is added
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13
Q

what does t-rna do?

A

when codons are “read out” from the mrna , trna with the matching anticodon brings the respective amino acid to the site of protein synthesis.

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14
Q

What happens in transcription ?

A

Sections of DNA are unwound using RNA polymerase, exposing a group of nucleotides.
Complementary bases assemble opposite to the temple strand
Pre mRNA is synthesised
Introns are spliced off from the final mRNA which travels to the ribosome

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15
Q

Where does transcription happen?

A

5’end -3’ end

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16
Q

What is translation?

A

mRNA formed previously in transcription is transported
tRNA away

When codons are read out in the ribosome the tRNA with the complementary anticodon brings the respective amino acid to the site of synthesis

17
Q

What doe the message from mRNA have?

A

Start and stop codons

Condons for respective proteins

18
Q

What is a tRNA

A

A type of molecule which have an anticodon at one end and a specific amino acid attachment at the other end .

19
Q

Can’t tRNA with different anti codons code for the same amino acid ?

A

Yes because of the degenerate nature of the triplet code.

20
Q

Prokaryotes have extra chromosomal DNA in

A

Plasmids

21
Q

Euks have extra chromasomal material in

A

Mitochondria

Chloroplast

22
Q

Chromosome - present or absent in pros and Euks

A

Pros - absent usually

Euks- present.

23
Q

What is the start codon ?

A

Tac

24
Q

Stop codon (RNA)

A

UAA
UAG
UGA