Proteins Flashcards
(30 cards)
Amino acid with sulfur
Cys and methionine
Which amino acid can for disulfide bond
Cys only
Pk1 pk2 and pkr
pI of acid and base
Acid= pk1+pr/2
Base=pk2+pr/2
Histadine side chain
Imidazole ring
Basic amino acids
Histidine
Lysine
Arginine
Acidic amino acid
Aspartic acid
Glutamic acid
Uncharged polar amimo acid
Aspargine
Glutamine
Tyrosine
Hydrophobic amino acid
Glycine
Alanine
Technique used to synthesis Protein and the dir of protein syn
Solid phase
C to N
Most amino acid present in L/D and S/R
With exception
Mostly amino acids L and S
Exception cysteine-R
Cleavage site or ctbr
C of met
Cleavage site of trypsin
Lys and arginine - Cend
K and R
Cleavage site of chymotrypsin
Tyrosine, phenylalanine and tryptophan
Y, F, W C end
Cation exchanger, charge and example
Charge- negative
Eg
Anion exchanger charge and example
Anione ex- Positive
Eg
In Size exclusion chromatography what elute first
Large particle
Stationary phase if reverse phase chromatography
Hydrophobic
Alpha gelix can be
3.6 10
Alpha helix measurements
Xyz
Beta sheets measurement
Dist bbt 2 amino acid- 3.4?
Micheal menten eq
Vo=Vmax×[S]/Km+[S]
Competitive en binds at
, And changes in vmax and Km
Active site
Km reduces
vmax same
Non competitive binds and effect on Km and Vmax
Binds at regulatory site
Uncompetitive enzyme binds at, and effect on km and vmax
Xyz
