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Flashcards in Proteins to Know Deck (30):
1

DNA Polymerase

Assist in the formation of molecules
Acts as a catalyst by assisting in splitting DNA

2

DNA Polymerase I

Replication
Removes RNA primer, fills nucleotides 5'-3'
Reads shape and polarity of incoming dNTP

3

DNA Polymerase II

DNA repair
3'-5' exonuclease activity

4

DNA Polymerase III

Replication of leading and lagging strand, 3'->5'

5

Helicase

Responsible for unwinding DNA, forms replication fork

6

Clamp Loader

Loads clamp onto DNA, uses ATP hydrolysis and helps DNA polymerase stay on DNA through the entire cycle of replication

7

elF4e

eukaryotic translation initiation factor, aids initiation by recruiting ribosomes at 5' cap

8

eRFI

Release factor, recognizes stop codon

9

Ori

signals for origin of replication, bacteria have single origin, eukaryotes have multiple origins

10

PABP

RNA binding protein, binds to poly(A) tail of mRNA before translation

11

RRF

ribosome recycling factor, recycles and splits ribosome int subunits, releasing mRNA

12

SIgma Factor

needed for transcription initiation, enables binding of RNA poly to gene promoters

13

TBP

TATA binding protein, general transcription factor that binds specifically to a DNA sequence called TATA box

14

eIF4G

eukaryotic translation initiation factor 4G, directly binds to mRNA cap, found in cytoplasm and nucleus

15

GAPs

GTPase activating proteins, bind to G proteins and stimulate GTPase activity

16

GEFs

Guanosine nucleotide exchange factors, activate monomeric GTPases by stimulating the release of guanosine diphosphate

17

Topoisomerase

over/underwinding of DNA, bind to DNA and cut the phosphate backbone

18

EF-G

Elongation factor, translocase. GTPase responsible for catalyzing movement of tRNA and mRNA

19

EF-Tu

Elongation factor thermos stable, prokaryotic elongation factor responsible for catalyzing the binding of aminoacyl tRNA to the ribosome, one of the most conserved protei

20

Drosha

Class 2 ribonuclease III enxyme, participate in RNA maturation and decay pathways, executes initiation step of microRNA

21

Dicer

trims dsRNA to from microRNA, targets mRNA to prevent translation

22

RNA Polymerase

release RNA transcript at specific DNA sequences encoded at the end of genes, products include mRNA, template for synthesis of protein by ribosomes

23

Hfq

abundant bacterial RNA binding protein, regulate transcription and translation factors, intreacts with sRNA

24

ORC

multisubunit DNA binding complex, binds all eukaryotes in ATP dependant manner, required for initiation, directs DNA replication

25

Argonaute

Role in RNA silencing, RISC complex, binds different classes of small non-coding RNAs, including microRNAs, siRNAs and piRNAs

26

TFII complex

TFIID and TFIIB, make up RNA Poly II complex, binds to TATA box before the start of transcription

27

IFI

Interferon inductible protein, localizes the nucleoplasm, interacts with p53

28

IF3

required for 30S subunit to bind to the initiation site in mRNA, prokaryotic initiation factor

29

DnaA

activates initiation of DNA replication in bacteria, promotes unwinding at oriC

30

GTPases

Large family of hydrolase enzymes that can bind and hydrolize ATP, highly conserved