Question Manuel Flashcards by Catherine Desjardins

Glycogen is best demonstrated by the use of:
Crystal violet colloidal iron
Mayer mucicarmine
Periodic acid-Schiff (PAS) with and without diastase
Alcian blue with and without Hyalaronidase

Periodic acid-Schiff (PAS) with and without diastase

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Amyloid can be demonstrated with
Congo red
Mayer mucicarmine
Crésyl echt violet
Alcian bleu

Congo red

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The Schiff réaction demonstrates:
Hyalaronidase acid
Aldehydes
Basophiles compounds
Amphoteric tissus components

Aldehydes

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A good controle for the Mayer mucicarmine stain is:
Kidney
Appendix
Spleen
Liver

Appendix

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À good controle for glycogen is:
Kidney
Appendix
Lung
Liver

Liver

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To increase the specificity for amyloid, Congo red stains should be examined by which of the following types of microscopy

Phase contrast
Dark field
Polarized
Electron

Polarized

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Of the following, which is the best fixative for glycogen
gluteraldehyde
Orth
acetic acid
absolute alcohol

Absolute alcohol

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Periodic acid uis used in the PAS technique as a/an:
Mordant
Stain
Reducer
Oxidizer

Oxidizer

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Acid mucopolysaccharides are demonstrated by:
Alcian bleu
Alizarin red
Congo red
Acid fuchsin

Alcian bleu

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The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of Schiff solution
Sodium iodate
Formaldehydes
Potassium Métabisulfite
Diastase

Formaldéhyde

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A fluorescent dye used for the demonstration of amyloid is:
Crystal violet colloidal iron
Auramine O
Colloidal iron
Thioflavin T

Thioflavin T

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The alcian blue stain performand at pH 1,0 demonstrates:
All acid mucopolysaccharides
Carboxylated acid mucopolysaccharides
Sulfatés acid mucopolysaccharides
Neutral mucopolysaccharides

Sulfatés acid mucopolysaccharides

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Substance stained positive with the colloidal iron procedure will be:
Red
Blue
Apple green
Violet

Blue

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Schiff reagent is a/an
Reduced solution of basic fuchsin
Oxidized solution of basic fuchsin
Reduced solution of acid fuchsin
Oxidized solution of acid fuchsin

Reduced solution of basic fuchsin

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Diastase digestion increased specificity for
Mucin
Amyloid
Carbohydrate
Glycogen

Glycogen

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Colloïdal iron is used for the demonstration of
Amyloid
Glycogen
Acid mucopolysaccharides
Neutral mucopolysaccharides

Acid mucopolysaccharides

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Glycogen may be removed from tissue by digestion with
A-amylase
Hyluronidase
Sialic acid
Chitin

A-amylase

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Adjacent sections are stained with PAS, one with and one without diastase digestion. A positive result on the one without digestion and a negative result on the one with digestion indicates the presence of:
Carboxylated mucosubstances
Sulfatés mucosubstances
Hyalaronic acid
Glycogen

Glycogen

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Which of the following fungi are well demonstrated with the colloidal iron stain?
Histoplasma capsulatum
Cryptococcus neoformans
Candida ablicans
Coccidioides immitis

Cryptococcus neoformans

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True or false
Glucose, sucrose and other oligopolysaccharides can be demonstrated easily in tissue sections

False

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True or false
Hyalaronidase is used to digest some connective tissue mucin

True

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True or false
The routine alcian blue stain is done at a pH of 1,5

False

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Good Schiff reagent should be light pink
True or false

False

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Amyloid shows a yellow biréfringence following staining with Congo red
True or false

False

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Glutaraldéhyde is one of the recommended fixatives for the PAS reaction True or false

False

The Schiff reaction may show false positivity following chromate-containing fixatives True or false

False

True or false Glycogen containing tissue fixed with Bouin solution may show resistance to a diastase digestion

True

No staining of the glomérular basement membrane can be seen microscopically on a control sections of kidney. This may be a result of: Inadequate oxidation Prolonged treatment with Schiff reagent Thickening of the basement membrane Lack of treatment with sodium Métabisulfite

Inadequate oxidation

Marked nonspecific background staining is noted on a section with the PAS technique. This could be a result of: Over oxidation of the section Prolonged washing with water Fixation with glutaraldéhyde Fixation with Zenker solution

Fixation with glutaraldéhyde

Very weak staining is noted on a PAS-staining control sections of liver. One problem solving action is: Check if Schiff reagent with formaldehyde Verify the use of potassium Métabisulfite Shorten the oxidation time Decrease the washing time

Check if Schiff reagent with formaldehyde

Sections of small intestine show orange goblet cells that are partially obscured by the yellow background. This is likely the result of: Prolonged staining with mucicarmine Overstraining with metanil yellow Improper use of iron Hématoxyline A port choice of control tissue

Overstraining with metanil yellow

Control sections stained with Congo red show only yellow and no green, biréfringence. This could probably be corrected in the future by Reducing the time in the Congo red show Using iron Hématoxylin for the nuclear stain Ensuring that the sections are cue at 8-10 um Using only control tissue fixed in formalin

Ensuring that the sections are cue at 8-10 um

With both the Masson and Gomori trichrome procedures, Muscle satins: Red Green Blue Yellow

Red

Which of the following méthodes best demonstrates elastic tissue Verhoeff-van Gieson Silver imprégnation Gomori thrichrome Periodic acid-Schiff (PAS)

Verhoeff-van Gieson

The first step in most réticuline methods is Silver imprégnation Sensitization Reduction Oxidation

Oxidation

The Verhoeff method differentiates with An acid A base An oxidizer Excess mordant

Excess mordant

Van Gieson solution stains collagène Green Blue Red Yellow

Red

Bouin solutions functions in the Masson trichrome stain as a/an: Oxidizer Decolorizer Reducer Mordant

Mordant

The components of van Gieson solution are: Picric acid and basic fuchsin Picric acid and acid fuchsin Aniline blue and Biebrich scarlet Light green and acid fuchsin

Picric acid and acid fuchsin

Most silver stains use which of the following as toning agent? Sodium Thiosulafte Formaldéhyde Uranyl nitrate Gold chloride

Gold chloride

The reducing agent in most réticulin stains is Formaldehyde Periodic acid Uranyl nitrate Sodium Thiosulafte

Formaldehyde

A good control for reticulin stains is Kidney Blood vessel Liver Skin

Liver

Toluidine blue is used to demonstrate which of the following cells? Plasma Mast Fibroblasts Macrophage

Mast

The component of basement membranes that is usually demonstrated with special stains is: Protein Carbohydrate Laminin Collagen

Carbohydrate

À stain that might be used to demonstrate cirrhosis of the liver is the Orcein Verhoeff-van Gieson Masson trichrome Méthénamine silver

Masson trichrome

In the Masson trichrome, Biebrich scarlet is removed from collagen by: Acetic acid Aniline blue Picric acid Phosphotungstic acid

Phosphotungstic acid

Iron Hématoxylin, rather than aluminium hemtoxylin, is usually used to satin nuclei in trichrome procedure because Subsequent staining solutions are acidic Of the staining solution stability The satin is more easily prepared The mounted sections are more permanent

Subsequent staining solutions are acidic

The preferred fixative for the Masson trichrome stain is: Bouin solution 10% neutral buffered formalin Glutaraldéhyde Absolute alcohol

Bouin solution

Silver imprégnation stains for reticulin depend on the formation of which of the following chemical groups? Quinoid Aldehyde Carboxyl Amino

Aldéhyde

Sections for the demonstration of basement membranes should be cut at 2 4 6 6

2 um

The oil red O stain requires which of the following sections: Paraffin Celloidin Frozen Plastic

Frozen

The oil red O stain might be used to demonstrate: rhabdomyosarcomas leiomyosarcomas liposarcomas adenocarcinomas

liposarcomas

Which of the following methods is an exemple of physical staining? Toluidine blue Verhoeff-van Gieson Méthénamine silver Oil red O

Méthénamine silver

Fat is chemically fixed and maintained in tissue by: Osmium tetroxide Mercuric chloride Calcium formol Carnoy solution

Osmium tetroxide

Plasma cells can be demonstrated with Methyl green-pyronin (MGP) Verhoeff-van Gieson Toluidine blue PAS

Methyl green-pyronin (MGP)

When ferric ammonium sulfate is used in a silver stain for reticulin, it functions as the Oxidizer Sensitizer Reducer Toner

Sensitizer

When used in a silver stain for reticulin, phosphomolybdic acid functions as the Oxidizer Sensitizer Reducer Toner

Oxidizer

The Verhoeff Van Gieson shows both orange collagen and orange muscle. This most likely could be corrected in the future by Différentiating longer in 95% alcool Ensuring that the Picric acid solution is saturated Increasing the concentration of the acid fuchsin Mordanting the section in Bouin solution

Ensuring that the Picric acid solution is saturated

The Masson trichrome stain shows only faint grayish pink staining of the muscle. This could most likely be prevented in the future by Increasing the concentration of phosphotungstic acid Using a mixture of phosphotungstic and phosphomolybdic acid Using fresh acid fuchsin-Biebrich scarlet solution Ensuring that the sections are acidic at the end of the stain

Using fresh acid fuchsin-Biebrich scarlet solution

Fat droplets are seen in the tissue space of an oil red P-staioned section. This most likely resulted from Dissolving the satin in propylene glycol Using glycerine jelly for mounting the sections Pressing on the cover glass to remove air bubbles A displacement of fat during paraffin processing

Pressing on the cover glass to remove air bubbles

Formalin fixed tissue shows very faded blue staining with the Masson thrichrome technique. The most likely explanation is that the sections were Left too long in the final acetic acid solution Stained with old acid fuchsin Fixed in Bouin solution Dried in a hot dryer

Left too long in the final acetic acid solution

Acid fast stains ,at best negative if the tissue was fixed in: 10% formalin B-5 solution Carnoy solution Zenker solution

Carnoy solution

In the fite méthode, the organisms stain: Red Blue Green Black

Red

After Schiff reagent, tissues are rinsed in a sulfite solution to: Fix the stain in the tissue Différentiate the stain Remove the excess leucofuchsin Oxidize the tissue

Remove the excess leucofuchsin

The property on which the acid fast stains depends is its: Affinity for anionic dyes Capability for reducing silver Failure to satin with a dye other than basic fuchsin Ability to resist decolorixation with dilute acids

Ability to resist decolorixation with dilute acids

Which of the following is a method for demonstration of fungi Ziehl Nielsen Warthin-starry Giemsa Periodic acid-Schiff (PAS)

Periodic acid-Schiff (PAS)

The best satin for the demonstration of mycobacterium leprae is the Fite PAS Kinyoun Gram

Fite

Artifactual precipitate seen in the Groccott stain may be the result of using Plastic forceps Gelatin in the water bath Glassware that was not chemically cleaned Excess time in the gold chloride solution

Glassware that was not chemically cleaned

Spherical or avoid bacteria are classified as Rickettsia Bacilli Spirochètes Cocci

Cocci

The carbol-fuchsin methods are specific for Mycobacteria Dimorphic fungi Diplococci Spore-forming bacilli

Mycobacteria

Carbol-fuchsin contains Acid fuchsin and HCL Acid fuchsin and phenol Basic fuchsin and HCL Basic fuchsin and phenol

Basic fuchsin and phenol

The term « mycosis » is used to describe a disease caused by Fungi Viruses Bacteria Rickettsiae

Fungi

Stains for the demonstration of spirochètes are based on their property of Argentophilia Argyrophilia Metachromasia Acid-fastness

Argyrophilia

Which of the following is a Méthénamine silver technique Ziehl Nielsen Grocott CAS Brown & brenn

Grocott

The PAS reaction will demonstrate fungi because the cell wall contains Lipids Carbohydrates Reducing substances Argyrophilic protein

Carbohydrate

The preferred fixative for the Warthin Starry technique is Carnoy Zenker B-5 10% buffered formalin

10% buffered formalin

The developper in the Warthin Starry stain is Gelatin Hydroquinone Pyridine Uranyl nitrate

Hydroquinone

If fungi are to be demonstrated, a good counter stain for the PAS technique is Nuclear fast red Van Gieson Light green Eosin

Van Gieson

An antigen is a substance that triggers the production of: Immunogens Immunoglobulins Hormones Epitopes

Immunoglobuline

A light chain present in some antibodies is IgM IgG IgE K

Différent molecular sites on antigens are known as Antibody classes Heavy chains Epitopes Immunoglobuline

Epitopes

When an antibody labelled with a chromogen reacts with tissue from a patient, the immunohistochemical technique is called Direct Indirect Avidin-biotin complex Soluble enzyme immune complex

Direct

Which of the following is a flurochrome Alkaline phosphatase Rhodamine Fast red Diaminobenzine

Rhodamine

Question Manuel Flashcards

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