Quiz 1

Question 1

What is physiological saline used for?

Answer 1

washing RBCs and diluting serum

Question 2

What is phosphate buffered saline used for?

Answer 2

when pH is important

Question 3

In an immunological titration, the highest dilution will be the one showing what?

Answer 3

agglutination, precipitation, hemagglutination, or color reaction

Question 4

Define diluate

Answer 4

the concentrated substance to be diluted

Question 5

Define diluent

Answer 5

substance making the dilution

Question 6

What is the formula for the dilution?

Answer 6

Diluate: diluate + diluent

Question 7

What is the dilution factor?

Answer 7

reciprocal of the dilution

Question 8

When preparing a desired volume of diluate, what is the formula to use?

Answer 8

desired volume / dilution factor = volume of diluate

Question 9

What is the formula for determining the amount of diluent needed?

Answer 9

amount of diluent * (df - 1)

Question 10

Why do we use serial dilutions?

Answer 10

to make a very concentrated solution very diluted

Question 11

When you have a certain dilution, but need to make a different one, what is the formula?

Answer 11

dilution you want / dilution you have = dilution you need to make

Question 12

If the dilution is 10% what parts of diluent and diluate would you need?

Answer 12

1 part diluate to 9 parts diluent

Question 13

What are the units of 1 Molar (1M)?

Answer 13

1 molecular weight / Liters

Question 14

How do you convert a dilution to a percentage?

Answer 14

divide 100 by df
multiply each side of the dilution by that number

Question 15

How to find the actually amount you have when given the volume and dilution?

Answer 15

multiply the volume by how much you have

Question 16

What is the formula is you know concentration and volume?

Answer 16

C1V1=C2V2

Question 17

What is a cell density dilution?

Answer 17

when the cell density is diluted to achieve a desired cell density

Question 18

How would you solve this problem?
How many mL of diluent would you add to 3mL of diluate to prepare a 1:X dilution?

Answer 18

3 mL * diluent(X-1)

Question 19

How would you solve this problem?
How many mL of diluate would you add to 27 mL of diluent to prepare a 1:X dilution?

Answer 19

27/(X-1)

Question 20

Why are mice used in the lab?

Answer 20

reproduce easily
many genetic strains
docile
cheap

Question 21

What does IP stand for?

Answer 21

intraperitoneally

Question 22

What does agglutination mean?

Answer 22

clumping resulting from antibodies binding to particular antigen

Question 23

What is used to determine ABO blood type?

Answer 23

hemagglutination

Question 24

Will the blood type contain the antigen for the same blood type?

Answer 24

yes

Question 25

What are isoantibodies?

Answer 25

antibodies produced in one member of a species with specificity for antigens found in another member of the same species

Question 26

Will the blood type contain isoantibodies for the same blood type?

Answer 26

no

Question 27

How do you know what the Rh factor is?

Answer 27

positive will agglutinate

Question 28

What is hemolytic disease of the newborn?

Answer 28

when Rh positive fetal red cells are introduced into an Rh negative mother's circulation at birth and can destroy Rh positive cells during subsequent pregnancies

Question 29

What does the indirect Coombs test look for?

Answer 29

anti-Rh antibodies in mother's blood

Question 30

What does the direct Coombs test look for?

Answer 30

maternal anti Rh antibodies attacking fetal RBC's

Question 31

What three blood cells have spots?

Answer 31

neutrophil, eosinophil, and basophil

Question 32

What two blood cells don't have spots?

Answer 32

lymphocyte and monocyte

Question 33

What type of blood cell has an organish tint?

Answer 33

eosinohpil

Question 34

What cells are most dense, and are at the bottom, after cetrifusion, of blood

Answer 34

erythorocytes and granulocytes

Question 35

What cells are the least dense, and at the top after cetrifusion, of blood?

Answer 35

plasma and plateltes

Question 36

What cells are right under the plasma and platelets after cetrifusion?

Answer 36

mononuclear cells

Question 37

What is the formula for the cells/mL?

Answer 37

average # cells in one square * 10^4 * df

Question 38

What is the formula for total cell number in tube?

Answer 38

(total cells counted/squares counted) * 10^4 * df * volume in tube

Question 39

What is a hemocytometer?

Answer 39

dual chamber microscope slide used to enumerate cells in suspension

Question 40

What is the volume of each square in a hemocytometer?

Answer 40

10^-4 mL