QUIZ 1

Question 1

True or false

Must be shipped in thick glass or plastic culture bottles

Answer 1

true

Question 2

True or false

Properly sealed with waterproof tape and packed with
absorbent material

Answer 2

true

Question 3

True or false

Labeled and with biohazard symbol

Answer 3

true

Question 4

True or false

Placed in second container (metal tube)

Answer 4

true

Question 5

True or false

Cardboard mailing tube is labeled with etiologic agent
label & biohazard symbol

Answer 5

true

Question 6

Which blood to broth ratio is most useful to for culture?
* a. 1:1
* b: 1:2
* c. 1:10
* d. 10:1

Answer 6

c. 1:10

Question 7

Specimen Transport

for urine colony counts

Answer 7

for urine colony counts

Question 8

True or false

At least two patient identifiers should be used to positively identify a patient prior to specimen collection.

Answer 8

true

Question 9

True or false

Uterine cervical swab in Cary-Blair transport medium is accepted for bacterial
culture.

Answer 9

True

Question 10

True or false

Urine sample stored at 4°C for 24 hours is accepted for bacterial culture.

Answer 10

True

Question 11

Lower respiratory tract specimens include the following, except:
* a. Sputum
* b. Bronchial washings
* c. Bronchoalveolar lavage
* d. Nasopharyngeal swabs

Answer 11

d. Nasopharyngeal swabs

Question 12

used for stool and O&P

Answer 12

PVA or Buffer formalin

Question 13

used for stool culture if its delayed by more than >20hrs

Answer 13

Cary-Blair transport medium

Question 14

holding mediums

Answer 14

Stuart’s medium and Amie’s medium

Question 15

selective agar and CO2 generating tablets

Answer 15

JEMBEC System

Question 16

absorbs fatty acid taht alters the PH changes in culture media

Answer 16

Charcoal

Question 17

anticoagulants

most common anti coagulant in microbiology

Answer 17

0.025% w/v Sodium polyanethol sulfonate (SPS)

Question 18

anticoagulants

for viral culture and microbacterium spp from blood

Answer 18

Heparin

Question 19

Specimen Storage

Refrigerator Temp

Answer 19

4°C

Question 20

Specimen Storage

Ambient/Room Temp

Answer 20

22°C

Question 21

Specimen Storage

Body Temp

Answer 21

37°C

Question 22

Specimen Storage

Freezer Temp

Answer 22

-20°C or -70 °C

Question 23

Specimen handling

Superficial
Abscess

Answer 23

Container : Aerobic swab with stuart’s or amie’s medium

Patient preparation : wipe the area with 70% alcohol

Instruction : aspirate if possible; Swab along edge of wound

Transport Time : <2 hrs

Storage Before Processing : 24hrs/Room tempreture

Question 24

Specimen handling

Deep Abscess

Answer 24

Container : Anaerobic Transporter

Patient preparation : Wipe area with sterile saline or 70% alcohol

Instruction : Aspirate/excise tissue

Transport Time : <2 hrs

Storage Before Processing : 24hrs/Room tempreture

Question 25

# Specimen handling Bone Marrow Aspirate or Blood

Answer 25

Container : Blood Culture Media Set (aerobic/anaerobic/Vacutainer Tube with SPS) Patient preparation : Disinfect puncture site with 70% alcohol in circular outward motion. Followed by : chlorhexidine/iodophor scrub left for 1 min then 2nd alcohol rinse Instruction : Draw blood at febrile phase; Draw 2 sets (from right and left arm); Amount depends on manufacturer’s instructions Transport Time : Within 2 hrs/Room Tempreture Storage Before Processing : <2 hrs/RT Incubated at 37C once received and logged in the lab

Question 26

# Specimen handling CSF

Answer 26

Container : Sterile, screw cap tube ≥ 1 mL bacteria, virus ≥ 2mL fungi, AFB Patient preparation : Disinfect with iodine/chlorhexidine before collection Instruction : Transport Time : <15 mins/Room tempreture Storage Before Processing : <24 hrs Incubation at 37C except for viruses (4C up to 3 days)

Question 27

# Specimen handling Urethra

Answer 27

Container : Swab moistened with Stuart’s/Amie’s medium, JEMBEC Transport System Patient preparation : Collect 1 hr after patient’s last urination. Instruction : Remove exudate from urethral opening Transport Time : <2hrs/RT Storage Before Processing : 24 hrs/RT

Question 27

# Specimen handling Rectal Swabs

Answer 27

Container : Swab placed in enteric transport medium (Cary-Blair) Patient preparation : Instruction : Insert swab ~ 1-1.5 cm past anal sphincter Feces should be visible on swab. Transport Time : <2hrs/Room tempreture Storage Before Processing : <24 hrs Room Tempreture

Question 28

# Specimen handling Stool Culture

Answer 28

Container : Clean, leakproof containerTransfer feces to enteric transport medium if transport will exceed 60 mins Patient preparation : Instruction : For routine culture(Salmonella, Shigella, Campylobacter, etc.) Transport Time : Within 24 hrs/RT holding time Unpreserved <1hr/RT Storage Before Processing : 24 hrs 4C <48 hrs RT or 4C

Question 28

# Specimen handling IV, Catheters, Pins

Answer 28

Container : Sterile, screw-cap container ≥ 1 mL bacteria, virus ≥ 2mL fungi, AFB Patient preparation : Disinfect skin with alcohol before removal Instruction : IV catheters are cultured quantitatively by rolling the segment back and forth on agar with sterile forceps 4x Transport Time : <15 mins/Room tempreture Storage Before Processing : Plate ASAPStore at 4C for <2hrs

Question 29

# Specimen handling Vagina

Answer 29

Container : Swab moistened with Stuart’s/Amie’s medium or JEMBEC Transport System Patient preparation : Instruction : Remove exudateIf smear is needed, use a 2nd swab Transport Time : <2hrs/RT Storage Before Processing : 24 hrs/RT

Question 30

# Specimen handling Prostate

Answer 30

Container : Swab moistened with Stuart’s/Amie’s medium Sterile screw-cap tube Patient preparation : Clean urethral meatus with soap & water. Massage the prostrate through the rectum. Instruction : Collect secretions on swab or in tube. Transport Time : <2 hrs/RT for swab; Immediate if tube/RT Storage Before Processing : 24 hrs/RT for swab Plate immediately if in tube

Question 31

# Specimen handling BAL, BW

Answer 31

Container : BAL, BW Patient preparation : Sterile, screw-top container Instruction : Anaerobic culture if sheathed catheter was used Transport Time : <2hrs/RT Storage Before Processing : 24 hrs/RT

Question 32

Sputum, Tracheal Suction

Answer 32

Container : Sterile, screw-top container Patient preparation : First Morning Sx; Instruct Px to brush their teeth/rinse or gargle with water prior collection Instruction : Collect deep cough; Watery sample indicates presence of saliva * Expectorated * Induced Sputum Transport Time : <2hrs/RT Storage Before Processing : 24 hrs/RT