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Microbiology lab > quiz 4-15-15 > Flashcards

quiz 4-15-15 Flashcards

(45 cards)

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1
Q

what type of charge do acid dyes have

A

a negative charge

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2
Q

what is the waxy lipid layer that some bacteria cells have in their cell walls

A

mycolic acid

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3
Q

what is the purpose of the mycolic acid

A

to protect the bacteria from drying out, protect it from disinfectants and the immune system

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4
Q

which cells do the safranin color, vegetative or capsules

A

vegetative cells

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5
Q

give an example of an acid fast bacteria

A

mycobacteria and nocardia, they are both rod shaped

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6
Q

in what phase of TB is the person coughing up bloody mucus, having night sweats, and increased fever

A

the active phase

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7
Q

in what phase of TB can become full blown in immuno compromised people, that have been exposed to the bacteria but initially were not showing any signs

A

latent TB

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8
Q

what is the oxygen requirement, arrangement and motility of mycobacterium

A

obligate aerobe, non-motile, rod shaped

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9
Q

how long can organisms remain alive in dried sputum from mycobacterium

A

6 months

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10
Q

how much of the worlds population has TB

A

1/3 of the world

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11
Q

what is the the name of the test that uses heat fixed and time to force the stain into the waxy cell walls

A

ziehl-neelsen acid fast stain

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12
Q

in the ziehl-neelsen acid fast stain what is the decolorizor used

A

acid alcohol

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13
Q

in the ziehl-neelson acid fast stain what is the name of the primary stain

A

Carbol fuchsin

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14
Q

in the ziehl-neelson acid fast stain what is the counterstain

A

methylene blue

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15
Q

how long is the slide heated for the ziehl-neelson acid fast stain

A

5 minutes

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16
Q

after the primary stain is applied and the decolorizer is used, what color are the non acid fast bacteria in the ziehl-neeson acid fast stain

A

pink

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17
Q

after the counterstain is applied in the ziehl-neelson acid fast stain, what color are the non-acid fast bacteria

A

blue

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18
Q

what color would mycobacteria be after the ziehl-neelson acid fast stain was complete

A

they would be blue, because they are non acid fast, they resist the decolorizer

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19
Q

what is used during the kinyoun method to force the carbol fuchsin into the waxy cell wall

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A

it uses a wetting agent, and a higher concentration of the carbol fuchsin

20
Q

what is the decolorizer in the kinyoun cold method

21
Q

what is the counterstain in the kinyoun acid fast stain

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A

brilliant green

22
Q

what color are the acid fast bacteria after applying the carbol fuchsin, during the kinyoun acid fast method

23
Q

what color are the non acid fast bacteria after the kinyoun acid fast stain

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A

brilliant green

24
Q

what is the route of transmission for the trypanosoma brucei

Study These Flashcards
A

bite from a tsetse fly

25
what is the mode of transmission for trypanosoma cruzi
bite from the kissing bug
26
what is the motility of trypanosoma cruzi and trypanosoma brucei
flagella
27
what is the infective stage for trypanosoma brucei and cruzi
trypomastigote
28
what is the definative host in the trypanosoma brucei and trypanosoma cruzi
the human
29
what is the intermediate host in trypanosoma brucei
the tsetse fly
30
what is the intermediate host for trypanosoma cruzi
the kissing bug
31
what disease is caused by the trypanosoma brucei
african sleeping sickness
32
what is the sickness that is caused by tropanosoma cruzi
chagas disease- south american
33
what is the mode of transmission of the leishmania donovani
flagella
34
what is the motility of the leishmania donovani
flagella
35
what is the infective stage of the leishmania donovani
promastigote
36
what is the definative host of the leishmania donovani
the human
37
what is the intermediate host of leishmania donovani
the sand fly
38
what is the disease caused by leishmania donovani
kala azar
39
what is the mode of transmission for cryptosporidium parvum
fecal oral ingestion of the oocyst
40
what is the motility of the cryptosproridium parvum
none they are amicomplexa
41
what is the infective stage of the cryptosporidium parvum
oocyst
42
what is the host of the cryptosporidium parvum
humans and other animals
43
what disease is caused by cryptosporidium parvum
cryptosproidiosis
44
what is the name of the mode of transmission for the toxoplasma gondii
ingestion of the cyst transferred from the mother to the fetus
45
what type of charge do capsules have
a negative charge

Microbiology lab (12 decks)

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