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Molecular Genetics > Quiz 4/18/23 > Flashcards

Quiz 4/18/23 Flashcards

1
Q

What enzyme does the chromatin remodeling complex have?

A

Contains DNA translocases

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2
Q

DNA translocases has a catalytic _______________

A

ATPase subunit

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3
Q

SWI/SNF family is a part of which complex?

A

Chromatin remodeling complex

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4
Q

ATP-dependent chromatin remodeling does what three things?

A
  1. Change in nucleosome position
  2. Histone eviction
  3. Replacement with histone variants (histone dimer exchange)
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5
Q

What are histone variants?

A

Mutated histone proteins

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6
Q

All histones have variants except

A

Histone H4

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7
Q

What happened to H1

A

Is a linker histone that is not part of the complex

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8
Q

What are the outcomes of chromatin remodeling?

A
  1. Repositioning
  2. Eviction
  3. Dimer exchange
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9
Q

Define Histone code hypothesis

A

Transcription is partly controlled by chemical modifications of histone proteins

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10
Q

The pattern of histone modifications act like a ____ or ____________

A

code, language

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11
Q

Where do covalent modifications occur?

A

On amino-terminal tails of histones

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12
Q

Histone modification directs certain ____________ in ______________________

A

alterations, chromatin structure

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13
Q

What does ChIP-Seq stand for?

A

chromatin immunoprecipitation sequence

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14
Q

The ChIP-Seq (chromatin immunoprecipitation sequence) allows for the determination of what three things?

A
  1. Nucleosome location
  2. Location of histone variants
  3. Location of where covalent modification of histones occurs
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15
Q

What is the procedure of ChIP-Seq (6 steps)

A
  1. Treat cells with formaldehyde to crosslink between DNA and histone of nucleosomes
  2. Ruptrue the cell and treat with micrococcal nuclease, which cuts linkers from nucleosomes
  3. Add histone antibody-treated bead to precipitate nucleosomes
  4. Isolate linker DNAs or nucleosome DNAs via centrifugation and electrophoresis
  5. Add short and known DNA sequences at both ends of isolated DNA
  6. Run PCR
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16
Q

The positioning of the nucleosome is observed to have what type of arrangement?

A

Typical arrangement pattern

17
Q

Transcriptional activation occurs through the modification of which protein?

A

histone protein

Molecular Genetics (5 decks)

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