quiz 5 Flashcards
most common approach to separating complex mixtures of biological
molecules.
chromotography
Differences in net charges of biological molecules are used to separate them.
Ion exchange chromatography (IEC).
Differences in net polarity of biological molecules are used to separate them
Hydrophobic interaction chromatography (HIC).
Highly specific and strong interactions like antigen-antibody, enzyme-substrate, etc. are used for separation
Affinity chromatography.
Separation achieved due to differences in hydrodynamic radius and shape
Size exclusion chromatography (SEC, gel filtration).
Beads ( chromotographic media) have _______ inside
pores
______ molecules travel through the void and inside the
pores. They exit the column last
small
_______ molecules travel through the void volume only and
exit the column bed first
large
total volume = volume pores + volume void
Vt= Vp + Vv
The extract containing the biomolecule(s) we’re
interested in is called the
mobile phase
Size exclusion chromatography (SEC) or gel-filtration relies on the ability of molecules to pass into the interior of the chromatographic medium
T/F
T
Everything that is _________ than the pore size will enter
the pores and travel through passages in the interior of the beads.
smaller
The space where beads are packed within
a column is commonly referred as the bed volume
The space where beads are packed within
a column is commonly referred as the
bed volume
The average pore diameter is called the __________, and
molecules smaller than this size must pass through both the interior of the beads and the void volume to reach the bottom of the column
exclusion limit
