Quiz Microscopy

Question 1

1

Answer 1

Main switch on/off

Question 2

2

Answer 2

Brightness adjustment knob

Question 3

3

Answer 3

Light-path_ selection-knob.

Question 4

4

Answer 4

Specimen holder

Question 5

5

Answer 5

Y-axis knob and x-axis knob

Question 6

6

Answer 6

Revolving nosepiece

Question 7

7

Answer 7

Coarse focusing knob and fine focusing knob

Question 8

8

Answer 8

Binocular portion

Question 9

9

Answer 9

Diopter adjustment rings

Question 10

Lo

Answer 10

Aperture diaphragm lever

Question 11

11

Answer 11

Fitter holder (for darker observation)

Question 12

12

Answer 12

Filter C the filter of 45mm in diameter can be inserted)

Question 13

Description of the microsvcope.
A microscope is a magnifying glass.

Question 14

A microscope is a magnifying glass

Answer 14

The compound light microscope ( brightfield microscope) the type use in most clinical laboratories consists of two magnifying lenses:
The objective and the eyespieces (ocular)

The total magnification observed is the product of the magnifications of these two lenses.

Question 15

Resolving power is the limit of usable magnification.

Answer 15

Resolution indicates how small and how close individual objects ( dots) can be and still be recognizable.

Question 16

Numerical aperture (NA) is an index or measurement of resolving power.

Answer 16

the light gathering ability of a microscope objective is quantitatively expressed in terms of the NA, which is a measure of the number of highly diffracted image forming light rays captured by the objectgive. Higher NA values allow increasingly oblique rays to enter the objective front lens, producing a more highly resolved image.

Question 17

Focal length

Answer 17

is the distance from the object being viewed to the lens or the objective.

the magnification and NA are inscribed on each lens as a number.

Question 18

parts of the mictoscope
Framework

Answer 18

Base
Arm
Stage

Question 19

Parts of the Microscope

Illumination system components

Answer 19

◦Light source, intensity control, types of bulbs
◦The condenser directs and focuses the beam of light from the bulb onto the material under examination.
◦The aperture iris diaphragm is located at the bottom of the condenser, under the lenses but within the condenser body. By opening or closing the aperture iris diaphragm, you let more or less light into the field of view.
◦A field iris diaphragm is also present in better microscopes.

Question 20

. Magnification system components

Answer 20

Ocular is the eyepiece, a lens that magnifies the image formed by the objective.
◦Objectives: achromatic and planachromatic,
low-power (10X),
high-power (40X), and
oil-immersion (100X).
◦Objectives are responsible for primary image formation and help determine the quality of images the microscope is capable of producing.

Question 21

Focusing system

Answer 21

Interocular adjustment, left ocular adjustment, body tube motion: coarse and fine

Question 22

Passage of Light in Microscope

Lamp condenser

Answer 22

Lens projects an image of the light source onto the plane of the condenser diaphragm.

Question 23

Ocular lens

Answer 23

The eyepiece magnifies the image created by objective lens a second time and creates an image as if viewed close to the eye.

Question 24

Objective lens

Answer 24

Gathers light passing through a specimen and projects image into the body tube, the beginning of the magnification process.

Question 25

Substage lens system

Answer 25

Once light has passed through the lamp condenser and the field diaphragm, it passes through the condenser aperture and lens system below the specimen stage. The substage condenser concentrates light onto the specimen.

Question 26

Brightfield Microscopy

Brightfield illumination is

Answer 26

the common type of illumination for general laboratory work.

•Because the microscope is such a precision instrument and an important piece of equipment, it must be kept in excellent condition, optically and mechanically. It must be kept clean and aligned.

Question 27

Care and Cleaning of the Microscope

◦Cleaning the microscope exterior

Answer 27

Gauze or soft cloth moistened with cleaning agent, rubbed in circular motion, and dried immediately with Kimwipe.

Question 28

Cleaning optical lenses and oculars

◦Cleaning the objectives

Answer 28

Only use lens paper- NEVER KIMWIPES

Always begin with the 10X then 40X then Oil-immersion (100X)

Question 29

Cleaning the condenser

Answer 29

First blow away dust, lint, and dirt with an air-syringe. Polish the light source and condenser with lens paper only- KIMWIPES

Question 30

Cleaning the stage and adjustment knobs

Answer 30

Clean after each use with gauze or Kimwipe. Make sure all parts are dry.

Question 31

Maintenance and Storage of the CX33 Microscope 1

Answer 31

1. Do not leave stains or fingerprints on the lenses and filters. Cleaning is performed by gently wiping the lens or filter with a piece of clean lens paper (NEVER KIMWIPES).

Question 32

Maintenance and Storage of the CX33 Microscope 2

Answer 32

2. Wipe the portions other than the lens with a dry soft cloth (piece of gauze).

Question 33

Maintenance and Storage of the CX33 Microscope
3.

Answer 33

3. Store the microscope in a dry place or cover it with the dust cover.

Question 34

Alignment

Answer 34

When properly aligned, the microscope is adjusted in such a way that the light path through the microscope, from the light source to the eye of the observer, is correct.

Question 35

Light adjustment

Answer 35

Light adjustment is made before any focusing is done. The power supply is turned on and the light intensity adjusted to a bright but comfortable level. Light adjustment is further accomplished by raising and lowering the condenser and opening and closing the aperture iris diaphragm.

Question 36

Focusing

Answer 36

Coarse adjustment is first, then fine adjustment and additional light adjustment.

Question 37

Other Types of Microscopes
(Illumination Systems)

Darkfield microscope

Answer 37

◦Uses scattered light to view objects that appear light on a dark background

Question 38

Other Types of Microscopes
(Illumination Systems)

Differential interference-contrast microscope

Answer 38

◦Gives objects a three-dimensional appearance
Electron microscope
◦Provides greater magnification and resolution than conventional light microscopy
◦Two types: transmission and scanning electron microscopy

Question 39

Other Types of Microscopes
(Illumination Systems)

Electron microscope

Answer 39

Provides greater magnification and resolution than conventional light microscopy
◦Two types: transmission and scanning electron microscopy

Question 40

Other Types of Microscopes
(Illumination Systems)

Fluorescence microscope

Answer 40

◦Used to observe molecules that fluoresce, such as molecules coupled to antibodies for specific fluorescent labeling

Question 41

Other Types of Microscopes
(Illumination Systems)

Phase-contrast microscope

Answer 41

Used to increase the contrast in unstained specimens, such as a waxy cast in a urine specimen
◦The microscopist must be proficient in changing from brightfield to phase contrast, because in a given specimen, some structures are better visualized with phase contrast and others with brightfield.

Question 42

Other Types of Microscopes
(Illumination Systems)

Polarized and compensated polarized microscopes

Answer 42

Used to identify crystals in body fluids (such as synovial or joint)
◦As light passes through the specimen, some objects (such as crystals) can rotate the light.
◦An object that polarizes bends light so it can be visualized when viewed through crossed polarizers.
◦A compensator is added in compensated polarized microscopes to help differentiate certain crystals and for other applications.

Question 43

Digital Microscopy

Answer 43

Digital microscopy automatically locates and pre-classifies blood cells into categories; it digitizes images for electronic retrieval and sharing.
Digital cell morphology
◦New systems allow for remote review of a smear and storage of up to 20,000 slides with images in a database.

◦https://www.cellavision.com/en/