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quiz2 A Flashcards

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1
Q

This is considered as the bony tissue
a. Periosteum
b. Endosteum
c. Adipocytes
d.Trabeculae
e. None of the choices

A

Trabeculae

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2
Q

This site can be utilized for marrow aspiratión but no
core biopsy
a. Anterior superior iliac crest
b. Anterior medial surface of tibia crest
d. Sternum
c. Posterior Iliac
e. Two of the choices

A

Two of the choices

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3
Q

What part of the bone marrow apparatus prevents
bone coring?
a. Outer puncture canula
b.Obturator
c. Needle
d. Stylet
e. NOTA

A

Obturator

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4
Q

Why peripheral blood collected before BM procedure?
a.Peripheral blood is more reflective of cellularity
b. Peripheral blood depicts real time results more than
BM specimen
c. There is a false decrease of white blood cells due to
stress
d.There is a false increase of white blood cells due to
stress
e.Two of the choices

A

Two of the choices

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5
Q

All of the following statement is/are descriptive of a
confirmatory low cellularity using core biopsy?
a. Fibrotic, packed marrow, leukemic cells
b. Fibrotic, acellular, leukemic cells
c. Packed marrow & leukemic cells
d. Packed marrow & fibrotic
e.All of the choices

A

All of the choices

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6
Q

Why should blowing of BM marrow smears be
avoided?
a. Causes low cellularity
b. DDistorts morphology
c. Causes high cellularity
d. Affects staining properties
e. NOTA

A

DDistorts morphology

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7
Q

The following types of tube can be utilized for
Concentrate smears except
a. Westergreen Hematocrit Tube
b. Narrow-Bore Glass Tube
c. Wintrobe Hematocrit Tube
d. Narrow-Bore Plastic Tube
e. NOTA

A

Westergreen Hematocrit Tube

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8
Q

Majority of bone marrow cells are composed of which
type of cell?
a. Metamyelocytes
b. Lymphocytes
c. Blasts
d. Neutrophil
e. two of the choices

A

two of the choices

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9
Q

This test can indicate the availability of binding sites
on the transferrin
a. Serum Iron
b.TIBC
c. Serum Ferritin
d. % Saturation of TIBC
e. NOTA

A

TIBC

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10
Q

There will be an increase in its level in a cobalamin
deficiency
a. Hepcidin
b. Cobalamin Assay
d. Deoxyuridine Suppression Test
c.Methylmalonic Acid and Homocysteine Assays
e. NOTA

A

Methylmalonic Acid and Homocysteine Assays

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11
Q

The
following
conditions
has
an
increase
surface-volume ratio except
a. Sickle cells
b. Spherocytes
c. Target Cells
d. Reticulocytes
e. NOTA

A

Spherocytes

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12
Q

TRUE or FALSE: Anemic patients with hemoglobin
levels of <7g/dL will have a false positive result in
Dithionite Tube
Test.
a. True
b. False
c. Both A and B

A

False

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13
Q

What are the hemoglobin(s) that migrate together
with Hb A, based on the results below?
a. DGL
b. CEO
c. DGEO
d. CSAF
e. NOTA
14. This t

A

CEO

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14
Q

This test is used to differentiate fetal or neonatal
blood from maternal blood
a.Alkali Denaturation
b.Isopropanol Precipitation
c. Ascorbate Cyanide
de. Antiglobulin (Coombs) Test
e. Fluorescent Spot

A

Alkali Denaturation

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15
Q

The opening where cells enter
a.Aa. aperture
b.b.Orifice
c. Internal Electrode
d. External Electrode
e. Two of the choices

A

Aa. aperture

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16
Q

Arrange the following events in a Coulter Principle
accordingly
I.The impedance is translated into pulses
Il.A measured amount of sample volume is extracted by
the probe into a chamber
III.Dilute suspension of cells is drawn through the
aperture
IV.Individual cell passes through the electrical current
increasing the impedance (resistance)
V. Cells are suspended in an electrically conductive
diluent such as saline
16. Which of the following events comes first?
a. l
b. II
C. III
d. IV
e. V

A

II

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17
Q

Arrange the following events in a Coulter Principle
accordingly
I.The impedance is translated into pulses
Il.A measured amount of sample volume is extracted by
the probe into a chamber
III.Dilute suspension of cells is drawn through the
aperture
IV.Individual cell passes through the electrical current
increasing the impedance (resistance)
V. Cells are suspended in an electrically conductive
diluent such as saline
Which of the following events comes second?
a. l
b. II
C. III
d. IV
e. V

A

V

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18
Q

Arrange the following events in a Coulter Principle
accordingly
I.The impedance is translated into pulses
Il.A measured amount of sample volume is extracted by
the probe into a chamber
III.Dilute suspension of cells is drawn through the
aperture
IV.Individual cell passes through the electrical current
increasing the impedance (resistance)
V. Cells are suspended in an electrically conductive
diluent such as saline
Which of the following events comes third?
a. l
b. II
C. III
d. IV
e. V

A

III

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19
Q

Arrange the following events in a Coulter Principle
accordingly
I.The impedance is translated into pulses
Il.A measured amount of sample volume is extracted by
the probe into a chamber
III.Dilute suspension of cells is drawn through the
aperture
IV.Individual cell passes through the electrical current
increasing the impedance (resistance)
V. Cells are suspended in an electrically conductive
diluent such as saline

Which of the following events comes fourth?
a. l
b. II
C. III
d. IV
e. V

A

IV

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20
Q

Arrange the following events in a Coulter Principle
accordingly
I.The impedance is translated into pulses
Il.A measured amount of sample volume is extracted by
the probe into a chamber
III.Dilute suspension of cells is drawn through the
aperture
IV.Individual cell passes through the electrical current
increasing the impedance (resistance)
V. Cells are suspended in an electrically conductive
diluent such as saline
Which of the following events comes last?
a. l
b. II
C. III
d. IV
e. V

A

l

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21
Q

What is the lower threshold of RBCs?
a. 24-36 fL
b. 30-35 fL
c. 2-20 fL
d. NOTA

A

24-36 fL

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22
Q

The following can cause spurious increase of WBC
except
a. Nucleated RBCS
b. Clumping
c. Clotting
d. Unlysed RBCs

A

Clotting

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23
Q

Which principle has the ability to detect the interior
density of a cell?
a. Direct Current
b.Radio Frequency
c.. Magnitude
d. Amplitude

A

Radio Frequency

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24
Q
  1. This component of Flow Cytometry is used to collect
    weaker signals produced at 90-degree angle
    a. Blocker Bars
    b. Sensing Zone
    c.Photomultiplier Tubes
    d. Limiting Aperture
    e. NOTA
A

Photomultiplier Tubes

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25
This component of Flow Cytometry allows isolation of a specific cluster of cells for analysis a. Laser b. Fluorochromes c. Detectors d.Gating e. Sheath fluid
Gating
26
. This is considered as the bony tissue. A. Peritoneum B. Endosteum C. Adipocytes D. Trabeculae E. None of the choices
Trabeculae
27
How many percentages does adipocytes take in the bone marrow? A. 50% B. 75% C. 80% D. 100% E. None of the choices
50%
28
Which part of the bone marrow apparatus is embedded into the medullary cavity of the bone? A. Outer puncture cannula B. Obturator C. Needle D. Spilot E. None of the above
Outer puncture cannula
29
The following are composition of the Zenker fixative except. A. Potassium dichromate B. Sodium sulfate C. Sodium acetate D. Glacial acetic acid E. Two of the choices
Sodium acetate
30
All of the following are advantages of having bone marrow aspirate except A. Fast B. No need for decalcification C. Represents all cells D. Provides differential E. None of the above
Represents all cells
31
In what procedure can individual bony structures be transferred into the slide? A. Direct bone marrow aspirate smear. B. Anticoagulated aspirate smear. C. Crush smear. D. Wedge smear. E. None of the above.
Direct bone marrow aspirate smea
32
What is the ideal end time for Buffy Count Concentrate Centrifugation? A. 3,500 g for 15 minutes B. 2,500 g for 15 minutes C. 3,000 g for 10 minutes D. 2,500 g for 10 minutes E. None of the above
2,500 g for 10 minutes
33
What is the ideal stain for determination of hematopoietic cell structure. A. Prussian Blue B. Sudan Black B C. Wright Gemsa D. Acid Fast E. None of the above
Wright Gemsa
34
This is also considered as an acute phase reactant. A. serum iron B. TIBC C. serum ferritin D. percent saturation of TIBC E. none of the above
serum ferritin
35
This test will determine the index of the intravascular lifespan of the labeled RBCs. A. Erythrocyte survival studies B. Sucrose hemolysis test C. Osmotic fragility D. Autohemolysis E. None of the above
Erythrocyte survival studies
36
Which of the following has a decrease surface to volume ratio? A. Sickle Cells B. Spherocytes C. Target Cells D. Reticulocytes E. None of the above
Spherocytes
37
True or false? The use of whole blood can cause false negative and positive reaction in the cyanide tube dust. A. True B. False C. Both A and B
True
38
What are the hemoglobin that migrates together with hemoglobin S based on the results below? A. DGL B. CEO C. DGEO D. CSAF E. None of the above
None of the above
39
This test shows brown concentration in G6PD deficiency. A. Alkaline Denaturation B. Isopropanol Precipitation C. Ascorbate Cyanide D. Fluorescence Spot E. Agglutination Combs Test 14. This displays the pulses th
Ascorbate Cyanide
40
This displays the pulses that are generated by the cells. A. Voltage Pulse B. Volume Thresholds C. Oscilloscope D. Volume Distribution Histogram E. None of the above
Oscilloscope
41
What is the lower threshold for platelets? A. 24-36 fL B. 30-35 fL C. 2-20 fL D. None of the above
2-20 fL
42
The following can cause spurious decrease of RBC except A. giant platelets B. autoagglutination C. clotting D. microcytic red cells E. none of the above
giant platelets
43
What two measurements can be plotted against each other? A. Impedance vs. Conductivity B. RF vs. DC C. Magnitude vs. Impedance D. RF vs. Amplitude E. None of the above
Magnitude vs. Impedance
44
The following are characteristics of a laser light except A. enough intensity B. stable C. monochromatic D. isolated E. none of the above
none of the above
45
Leukocyte histogram flagging for Reactive Lymphocytes, Lymphoblasts, Basophils, and Clotted Samples is under what flag? A. R1 B. R2 C. R3 D. R4 E. Two of the choices
R2
46
The following are purposes for bone marrow examination except a. Stage both hematologic and non hematologic neoplasia b. Determine the cause of cytopenia c. Confirm and exclude metabolic conditions peripheral blood smear preparations d. Eradicate peripheral blood smear preparation e. Two of the choices
Eradicate peripheral blood smear preparation
47
This is the ideal site for children under 2 years of age a. Anterior superior iliac crest b. Anterior medial surface of tibia c. Posterior iliac crest e. Two of the choices
Anterior medial surface of tibia
48
How is core biopsy obtained? a. Arterial Puncture b. venipuncture C. Aspiration d. trephination e. NOTA
trephination
49
Why should the disposal of some of the fixatives be controlled? a. They have foul odor b. They contain toxic mercury c. They can cause erosion d. They can be acidic e. Two of the choices
They contain toxic mercury
50
Anticoagulant used for BM aspirate samples for smearing a.EDTA b. Heparin c. Sodium Citrate d. No anticoagulant e. NOTA
a.EDTA
51
What can be added to the solution if the specimen is suspected to contain prolymphocytes and lymphoblasts? a. 22% Albumin b. Heparin c. 3.2% Sodium Citrate d. EDTA e. NOTA
22% Albumin
52
Which of the following stains are used for identification of marrow cell maturation and stages? a. Prussian Blue b.. Sudan Black B c. Wright-Giemsa d. Acid Fast e. Two of the choices
Sudan Black B
53
This test can be an indicator of available transport iron a. Serum Iron b. TIBC c. Serum Ferritin d. % Saturation of TIBC e. NOTA
Serum Iron
54
This is a valuable tool for iron abnormalities a a.Hepcidin b. Cobalamin Assay c. Methylmalonic Acid and Homocysteine Assays d. Deoxyuridine Suppression Test e. NOTA
Hepcidin
55
This test will allow screening for PNH a. Erythrocyte Survival Studies b.Sucrose Hemolysis Test c. Osmotic Fragility d. Autohemolysis Test e. NOTA
Sucrose Hemolysis Test
56
What is the medium used in alkaline testing for Hemoglobin Electrophoresis? a. Citrate Agar b. Sodium Citrate c. Cellulose Acetate d. Cellulose Alkaline e. Two of the choices
two of the choices
57
This test is used to confirm diagnosis of PNH a Ham's Test b. Metabisulfite Slide Test c. Donath-Landsteiner d. Dithionite tube test e. Two of the choices
Ham's Test
58
What are the hemoglobin that migrate together with Hb A based on the results below? a. DGL b. CEO c. DGEO d. CSAF e. NOTA
DGEO
59
This test detects unstable Hb through precipitation a. Alkali Denaturation b. isopropanol Precipitation c. Ascorbate Cyanide d. Fluorescent Spot e. Antiglobulin (Coombs)
isopropanol Precipitation
60
All of the following can affect volume measurement except a. Deformability of RBC b. Persistent anemic conditions c. Aperture diameter d. Recirculation of cells e.bTwo of the choices
Persistent anemic conditions
61
The following can cause spurious decrease of HCT except a. Giant platelets b. Autoagglutination c. Clotting d. Hemolysis e. NOTA
Giant platelets
62
This component of Flow Cytometry maintains the particles in the center of the flowing stream a. Laser b. Fluorochromes c. Detectors heath fluid d. Gating e. sheath fluid
sheath fluid
63
This angle of light detects cellular structure and complexity a.90 b. 10 c. 0 d.depolarized 90 e. NOTA
10
64
Leukocyte histogram flagging for gránulocytosis a. R1 b. R2 c. R3 d.R4
R4
65
25. Which principle has the ability to detect the interior density of a cell? a. Direct Current b. Radio Frequency c. Magnitude d. Amplitude e. NOTA
Radio Frequency
66
This is the most ideal site for marrow aspirate and core biopsy a. Anterior superior iliac crest b. Anterior medial surface of tibia c. Posterior iliac crest d. Sternum e. Two of the choices
Posterior iliac crest
67
The procedure of acquiring bone marrow samples is often associated with what condition(s)? a. Cytopenia b. Hemorrhage c. Thrombocytopenia d. DIC e. Two of the choices
Two of the choices
68
Which of the following describes the purpose of bone marrow aspiration? a. Identify types and proportion of hematological cells b. BM architecture c. Estimate cellularity d. Morphological variance e. Two of the choices
e. Two of the choices
69
This is known is an unsuccessful collection of BM sample wherein no ample amount of needed material is acquired. a. Hemorrhagic Tap b. Traumatic Tap c. Wet Tap d. Dry Tap e. Two of the choices
Dry Tap
70
Anticoagulant used in direct aspirate smears a. EDTA b. Heparin c. Sodium citrate d. No anticoagulant e. NOTA
EDTA
71
This is the top most layer of a centrifuged marrow sample in Buffy Coat Concentrates a. Myeloid-erythroid b. Yellowish Fat c. Plasma d. RBCs e. NOTA
Yellowish Fat
72
What should be the proper course of action in staining thick marrow samples? a. Decalcify bony tissue b. Add more anticoagulant c. Increase staining time d. Decrease fatty substance e. NOTA
Increase staining time
73
This test can indicate the level of storage iron a. Serum Iron b. TIBC c. Serum Ferritin d. % Saturation of TIBC e. NOTA
Serum Ferritin
74
This measures the ability of marrow cells in vitro to utilize deoxyuridine in DNA synthesis a. Hepcidin b. Cobalamine Assay c. Methylmalonic Acid and Homocysteine Assays d. Deoxyuridine Suppression Test e. NOTA
Deoxyuridine Suppression Test
75
This test will aid in identifying Hereditary spherocytosis with the use of glucose a. Erythrocyte Survival Studies b. Sucrose Hemolysis Test c. Osmotic Fragility d. Autohemolysis Test e. NOTA
Autohemolysis Test
76
This test identifies the presence of sickle cells a. Ham’s Test b. Metabisulfite Slide Test c. Donath-Landsteiner d. Dithionite tube test e. Two of the choices
Metabisulfite Slide Test
77
What is the medium used in acid testing for Hemoglobin Electrophoresis a. Citrate Agar b. Sodium Citrate c. Cellulose Acetate d. Cellulose Alkaline e. Two of the choices
Two of the choices
78
What are the hemoglobin that migrate together with Hb Ca1 based on the results below? a. DGL b. CEO c. DGEO d. CSAF e. NOTA
NOTA
79
This test is used to detect the presence of antibodies against circulating RBCs a. Alkali Denaturation b. Isopropanol Precipitation c. Ascorbate Cyanide d. Flourescent Spot e. Antiglobulin (Coombs) Test
Antiglobulin (Coombs) Test
80
This displays the pulses that are generated by the cells a. Voltage Pulse b. Volume Thresholds c. Oscilloscopes d. Volume Distribution Histogram e. NOTA
Oscilloscopes
81
Why is RBC and Platelet aperture smaller than of WBC? a. To have an increase sensitivity towards WBC b. To have an increase sensitivity towards Platelets c. To prevent recirculation d. To prevent a decrease in aperture diameter that might cause erroneous results e. Two of the choices
To have an increase sensitivity towards Platelets
82
Which of the following events can cause a spurious increase of MCV? a. Giant platelets b. Autoagglutination c. Clotting d. Hemolysis e. NOTA
Autoagglutination
83
This component of flow cytometry is attached to the particles of the cells to be excited and create incident light a. Laser b. Flourochromes c. Detectors d. Gating e. Sheath fluid
Flourochromes
84
This angle of light detects cell size a. 90 b. 10 c. 0 d. Depolarized 90 e. NOTA
0
85
Leukocyte histogram flagging for reactive lymphocytes, lymphoblasts, basophils, and clotted sample a. R1 b. R2 c. R3 d. R4 e. Two of the choices
R2
86
The ideal site for patient in supine position.
Anterior superior iliac crest
87
Disadvantage of sternum
1 cm in thickness
88
Which of the following describes the purpose of your bone marrow for biopsy
bone marrow architecture; cellularity (2 of the choices)
89
Which of the statement is/are descriptive of an unsuccessful tap in bone marrow aspirate?
fibrotic, acellular, leukemic cells, packed marrow
90
Ideal length of bone marrow aspirate
1/2 or 3/4 length; 2-3 cm
91
Consists of nucleated cells:
Myeloid erythroid layer
92
Which stain allows estimation of marrow storage iron?
Serum ferritin
93
Test can indicate the number of transferrin sites:
TIBC
94
This will aid the recognition of megaloblastic anemia
Serum cobalamin assay
95
The acetic medium for hemoglobin erythropoiesis which hemoglobin migrates together?
ADGE
96
Migrate with hemoglobin S; acidic:
none
97
98
Other name for electrical impedance
resistance
99
Positive reaction for acid dilution:
pink red
100
The following can cause spurious increase of WBC, except:
flagging
101
Which principle has the ability to detect the inferior cell density?
radio frequency
102
The following can cause spurious increase of WBC, except:
flagging
102
Components of flocytometry that use to collect bigger signals produced at 90* angle:
Photo multiplier tubes
103
This component of flocytometry allows isolation:
gating
104
105
The following are characteristics of laser light, except:
NOTC
106
Angle of light detects outside
0
107
Angle of light detects cellular structured complexity
-10

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