Random Questions

Question 1

What are the virulence factors of Bacillus cereus

Answer 1

-Nonhemolytic enterotoxin
-Cytotoxin K
-Hemolysin BL

Question 2

Virulence factors of Bacillus anthracis

Answer 2

• D-glutamate capsule
• Exotoxin with 3 components (edema factor, lethal factor & protective antigen)

Question 3

A screening disk for Moraxella catarrhalis

Answer 3

Butyrate disk

Question 4

What is the third common cause of otitis media

Answer 4

M. catarrhalis

Question 5

Causes Ophthalmia neonatorum

Answer 5

Neisseria gonorrhoeae

Question 6

Major virulence factor of N. meningitidis

Answer 6

capsule

Question 7

Major virulence factor of N. gonorrhoeae

Answer 7

Pili

Question 8

The typical morphology of N. gonorrhoeae

Answer 8

kidney or coffee bean-shaped

Question 9

The reagent used in Modified Oxidase test

Answer 9

6% trimethyl-p-phenylene diamine dihydrochloride

Question 10

Result of
Bacitracin: Lysostaphin and Furazolidone between Micrococcus spp and Staphylococci spp

Answer 10

Micrococcus is SENSITIVE to Bacitracin and RESISTANT to Lysostaphin and Furazolidone;
Staphylococci is RESISTANT to Bacitracin and SENSITIVE to Lysostaphin and Furazolidone

Question 11

result of Neisseria spp in Oxidase test

Answer 11

Positive

Question 12

The agar used in CHO utilization test in Neisseria

Answer 12

Cystine Trypticase Agar

Question 13

True or False
Neisseria spp are cold-sensitive.

Answer 13

True

Question 14

What is the other name for gonorrhea

Answer 14

clap disease

Question 15

What is the color of a positive Oxidase test result for Neisseria

Answer 15

Purple

Question 16

How to destroy anthrax bacillus?

Answer 16

autoclaving at 121 deg Celsius

Question 17

Stain used in visualization of B. anthracis

Answer 17

Schaeffer and Fulton

Question 18

Name the primary dye and counterstain in Schaeffer and Fulton

Answer 18

Primary dye: Malachite green (stains spores)
Counterstain: Safranin (stains vegetative cells)

Question 19

Bamboo fishing rod on microscopy

Answer 19

Bacillus anthracis

Question 20

A selective agar used for Bacillus anthracis
colonies appear creamy white, domed

Answer 20

PLET- Polymyxin Lysozyme EDTA Thallous Acetate

Question 21

An agar used to detect lecithinase and lipase for Bacillus cereus

Answer 21

Egg Yolk Agar

Question 22

Klebs Loefller Bacillus

Answer 22

Corynebacterium diphtheriae

Question 23

Bull’s neck appearance

Answer 23

Corynebacterium diphtheriae

Question 24

Transmission pattern in C. diphtheriae

Answer 24

Person-to-person exposure to respiratory droplets

Question 25

Picket fence/pallisade/Chinese letters, x-y-v on microscope

Answer 25

C. diphtheriae

Question 26

The metachromatic granules of C. diphtheriae is called

Answer 26

Babes Ernst

Question 27

Gray to black colonies selective to c. diphtheriae

Answer 27

Cystine Tellurite Blood Agar *potassium tellurite also produces gray to black colonies

Question 28

This agar plate produces Black colonies with brown halo for C. diphtheriae

Answer 28

Tinsdale agar

Question 29

Hemolytic pattern of c. diphtheriae on BAP

Answer 29

beta-hemolytic

Question 30

It shows enhance granule and pleomorphism for C. diphtheriae, which agar is this?

Answer 30

Loeffler's medium

Question 31

What is the in vitro toxin test for Corynebacterium diphtheriae

Answer 31

Modified Elek's test

Question 32

What is the in vivo toxin detection test for Corynebacterium diphtheriae?

Answer 32

Animal inoculation/Guinea Pig Lethal Test

Question 33

What is the positive result of Modified Elek's test

Answer 33

Precipitin lines

Question 34

An immunity test for diphtheria

Answer 34

Schick's test

Question 35

Lavender to lavender green on BAP

Answer 35

Stenotrophomonas maltophilia (maltose positive)

Question 36

Purple on MAC

Answer 36

Acinetobacter

Question 37

wrinkled colonies

Answer 37

P. stutzeri and B. mallei

Question 38

A key pathogen in the lungs of cystic fibrosis patients

Answer 38

Pseudomonas aeruginosa

Question 39

beta-hemolytic, taco/corn tortilla/overripe grapes odor on BAP

Answer 39

P. aeruginosa

Question 40

The pigments produced by P. aeruginosa

Answer 40

pyocyanin (water-soluble blue) and pyoverdin (green)

Question 41

Biochemical characteristics of P. aeruginosa

Answer 41

- Nitrate reduction positive -LDC negative -Positive grown on Cetrimide agar -Positive growth in acetamide utilization

Question 42

Gelatin hydrolysis test on other Pseudomonas

Answer 42

Positive = P. fluorescens Negative = P. putida

Question 43

Causes onion bulb rot

Answer 43

Burkholderia cepacia

Question 44

Agent of Glandler's disease

Answer 44

Burkholderia mallei

Question 45

Earthy odor on Ashdown media

Answer 45

Burkholderia pseudomallei

Question 46

Corn-flower blue colonies on EMb

Answer 46

Acinetobacter baumannii

Question 47

Ammonia-like odor colonies

Answer 47

Acinetobacter baumanii

Question 48

Characteristics of Acinetobacter baumanii on MAC and BAP

Answer 48

MAC: blue colonies w peach to pink tint BAP: gummy colonies

Question 49

Causes abortion, febrile disease

Answer 49

Brucella

Question 50

Microscopy of Brucella

Answer 50

Gram negative Coccobacilli w sand appearance

Question 51

Media for isolation of Brucella

Answer 51

-Castañeda -Trypticase Soy agar -Tryptose agar -Brucella broth agar w Wisconsin media (5% horse or rabbit serum)

Question 52

Give the corresponding animal host of the ff. Brucella abortus Brucella melitensis Brucella suis Brucella canic

Answer 52

B. abortus = cows, camels B. melitensis = sheep B. suis = pigs B. canis = dogs

Question 53

Market men's disease, deerfly fever

Answer 53

Tularemia

Question 54

Susceptibility test for F. tularensis

Answer 54

Forshay test

Question 55

Causes whooping cough

Answer 55

Bordetella pertussis

Question 56

Causes septicemia and meningitis in men and kennel cough in dogs

Answer 56

Bordetella bronchiseptica

Question 57

characteristics of Bordetella pertussis on Bordet-Gengou medium

Answer 57

Mercury droplets

Question 58

Basic characteristics of Mycobacterium

Answer 58

Gram positive, Acid-fast, aerobic, and non-motile

Question 60

Most recommended plating media for B. pertussis

Answer 60

Regan-Lowe Charcoal Agar

Question 61

Composition of Regan-Lowe Chracoal agar

Answer 61

10% horse blood and cephalexin

Question 62

Hansen's disease

Answer 62

Leprosy

Question 63

Has microscopiic characteristics of cigar packet arrangement

Answer 63

Mycobacterium leprae

Question 64

What is the gold standard for digestion and decontamination

Answer 64

N-acetyl-L cysteine for digestion NaOH for decontamination

Question 65

What does the 3+ represent in Ziehl Neelsen-Kinyoun AF Staining?

Answer 65

More than 10 AFB/OIF in at least 20 VF

Question 66

Result of Fluorochrome staining for Mycobacterium

Answer 66

AFB: Yellow against black bbackground

Question 67

Which stains composes the Fluorochrome staining

Answer 67

Auramine rhodamine stain/TRUANT stain

Question 68

What does 3+ represent in Fluorochrome staining reporting

Answer 68

4-36 AFB/field

Question 69

What does 2+ represent in Fluorochrome reporting?

Answer 69

2-18 AFB/50 fields

Question 70

What does 4+ represent in Flurochrome reporting?

Answer 70

>36 AFB/field

Question 71

What is the reporting if there is 1-2 AFB/ 70 fields in fluorochrome staining?

Answer 71

Doubting, Repeat using new specimen

Question 72

In which tests is Lowenstein-Jensen Media being used for identifying Mycobacterium spp.?

Answer 72

In Niacin test and Iron Uptake test It is also used as a selective and non--selective media

Question 73

What reagent is used for the Iron Uptake Test of Mycobacterium?

Answer 73

Ferric Ammonium Citrate

Question 74

What is the color reaction of Arylsulfatase test?

Answer 74

pink