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Bacteriology > Random Questions > Flashcards

Random Questions Flashcards

1
Q

What are the virulence factors of Bacillus cereus

A

-Nonhemolytic enterotoxin
-Cytotoxin K
-Hemolysin BL

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2
Q

Virulence factors of Bacillus anthracis

A
  • D-glutamate capsule
  • Exotoxin with 3 components (edema factor, lethal factor & protective antigen)
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3
Q

A screening disk for Moraxella catarrhalis

A

Butyrate disk

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4
Q

What is the third common cause of otitis media

A

M. catarrhalis

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5
Q

Causes Ophthalmia neonatorum

A

Neisseria gonorrhoeae

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6
Q

Major virulence factor of N. meningitidis

A

capsule

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7
Q

Major virulence factor of N. gonorrhoeae

A

Pili

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8
Q

The typical morphology of N. gonorrhoeae

A

kidney or coffee bean-shaped

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9
Q

The reagent used in Modified Oxidase test

A

6% trimethyl-p-phenylene diamine dihydrochloride

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10
Q

Result of
Bacitracin: Lysostaphin and Furazolidone between Micrococcus spp and Staphylococci spp

A

Micrococcus is SENSITIVE to Bacitracin and RESISTANT to Lysostaphin and Furazolidone;
Staphylococci is RESISTANT to Bacitracin and SENSITIVE to Lysostaphin and Furazolidone

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11
Q

result of Neisseria spp in Oxidase test

A

Positive

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12
Q

The agar used in CHO utilization test in Neisseria

A

Cystine Trypticase Agar

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13
Q

True or False
Neisseria spp are cold-sensitive.

A

True

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14
Q

What is the other name for gonorrhea

A

clap disease

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15
Q

What is the color of a positive Oxidase test result for Neisseria

A

Purple

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16
Q

How to destroy anthrax bacillus?

A

autoclaving at 121 deg Celsius

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17
Q

Stain used in visualization of B. anthracis

A

Schaeffer and Fulton

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18
Q

Name the primary dye and counterstain in Schaeffer and Fulton

A

Primary dye: Malachite green (stains spores)
Counterstain: Safranin (stains vegetative cells)

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19
Q

Bamboo fishing rod on microscopy

A

Bacillus anthracis

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20
Q

A selective agar used for Bacillus anthracis
colonies appear creamy white, domed

A

PLET- Polymyxin Lysozyme EDTA Thallous Acetate

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21
Q

An agar used to detect lecithinase and lipase for Bacillus cereus

A

Egg Yolk Agar

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22
Q

Klebs Loefller Bacillus

A

Corynebacterium diphtheriae

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23
Q

Bull’s neck appearance

A

Corynebacterium diphtheriae

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24
Q

Transmission pattern in C. diphtheriae

A

Person-to-person exposure to respiratory droplets

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25
Q

Picket fence/pallisade/Chinese letters, x-y-v on microscope

A

C. diphtheriae

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26
Q

The metachromatic granules of C. diphtheriae is called

A

Babes Ernst

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27
Q

Gray to black colonies selective to c. diphtheriae

A

Cystine Tellurite Blood Agar
*potassium tellurite also produces gray to black colonies

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28
Q

This agar plate produces Black colonies with brown halo for C. diphtheriae

A

Tinsdale agar

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29
Q

Hemolytic pattern of c. diphtheriae on BAP

A

beta-hemolytic

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30
Q

It shows enhance granule and pleomorphism for C. diphtheriae, which agar is this?

A

Loeffler’s medium

31
Q

What is the in vitro toxin test for Corynebacterium diphtheriae

A

Modified Elek’s test

32
Q

What is the in vivo toxin detection test for Corynebacterium diphtheriae?

A

Animal inoculation/Guinea Pig Lethal Test

33
Q

What is the positive result of Modified Elek’s test

A

Precipitin lines

34
Q

An immunity test for diphtheria

A

Schick’s test

35
Q

Lavender to lavender green on BAP

A

Stenotrophomonas maltophilia (maltose positive)

36
Q

Purple on MAC

A

Acinetobacter

37
Q

wrinkled colonies

A

P. stutzeri and B. mallei

38
Q

A key pathogen in the lungs of cystic fibrosis patients

A

Pseudomonas aeruginosa

39
Q

beta-hemolytic, taco/corn tortilla/overripe grapes odor on BAP

A

P. aeruginosa

40
Q

The pigments produced by P. aeruginosa

A

pyocyanin (water-soluble blue) and pyoverdin (green)

41
Q

Biochemical characteristics of P. aeruginosa

A
  • Nitrate reduction positive
    -LDC negative
    -Positive grown on Cetrimide agar
    -Positive growth in acetamide utilization
42
Q

Gelatin hydrolysis test on other Pseudomonas

A

Positive = P. fluorescens
Negative = P. putida

43
Q

Causes onion bulb rot

A

Burkholderia cepacia

44
Q

Agent of Glandler’s disease

A

Burkholderia mallei

45
Q

Earthy odor on Ashdown media

A

Burkholderia pseudomallei

46
Q

Corn-flower blue colonies on EMb

A

Acinetobacter baumannii

47
Q

Ammonia-like odor colonies

A

Acinetobacter baumanii

48
Q

Characteristics of Acinetobacter baumanii on MAC and BAP

A

MAC: blue colonies w peach to pink tint
BAP: gummy colonies

49
Q

Causes abortion, febrile disease

A

Brucella

50
Q

Microscopy of Brucella

A

Gram negative
Coccobacilli w sand appearance

51
Q

Media for isolation of Brucella

A

-Castañeda
-Trypticase Soy agar
-Tryptose agar
-Brucella broth agar w Wisconsin media (5% horse or rabbit serum)

52
Q

Give the corresponding animal host of the ff.
Brucella abortus
Brucella melitensis
Brucella suis
Brucella canic

A

B. abortus = cows, camels
B. melitensis = sheep
B. suis = pigs
B. canis = dogs

53
Q

Market men’s disease, deerfly fever

A

Tularemia

54
Q

Susceptibility test for F. tularensis

A

Forshay test

55
Q

Causes whooping cough

A

Bordetella pertussis

56
Q

Causes septicemia and meningitis in men and kennel cough in dogs

A

Bordetella bronchiseptica

57
Q

characteristics of Bordetella pertussis on Bordet-Gengou medium

A

Mercury droplets

58
Q

Basic characteristics of Mycobacterium

A

Gram positive, Acid-fast, aerobic, and non-motile

59
Q
A
60
Q

Most recommended plating media for B. pertussis

A

Regan-Lowe Charcoal Agar

61
Q

Composition of Regan-Lowe Chracoal agar

A

10% horse blood and cephalexin

62
Q

Hansen’s disease

A

Leprosy

63
Q

Has microscopiic characteristics of cigar packet arrangement

A

Mycobacterium leprae

64
Q

What is the gold standard for digestion and decontamination

A

N-acetyl-L cysteine for digestion
NaOH for decontamination

65
Q

What does the 3+ represent in Ziehl Neelsen-Kinyoun AF Staining?

A

More than 10 AFB/OIF in at least 20 VF

66
Q

Result of Fluorochrome staining for Mycobacterium

A

AFB: Yellow against black bbackground

67
Q

Which stains composes the Fluorochrome staining

A

Auramine rhodamine stain/TRUANT stain

68
Q

What does 3+ represent in Fluorochrome staining reporting

A

4-36 AFB/field

69
Q

What does 2+ represent in Fluorochrome reporting?

A

2-18 AFB/50 fields

70
Q

What does 4+ represent in Flurochrome reporting?

A

> 36 AFB/field

71
Q

What is the reporting if there is 1-2 AFB/ 70 fields in fluorochrome staining?

A

Doubting, Repeat using new specimen

72
Q

In which tests is Lowenstein-Jensen Media being used for identifying Mycobacterium spp.?

A

In Niacin test and Iron Uptake test
It is also used as a selective and non–selective media

73
Q

What reagent is used for the Iron Uptake Test of Mycobacterium?

A

Ferric Ammonium Citrate

74
Q

What is the color reaction of Arylsulfatase test?

A

pink

Bacteriology (4 decks)

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