rate of enzyme controlled practical

Question 1

which enzyme is used in this practical and why?

Answer 1

• trypsin, as it hydrolyses peptide bonds and produced in small intestine

Question 2

what is the aim of the enzyme controlled reaction prac?

Answer 2

To investigate the effect of temperature on the rate of an enzyme-controlled reaction using trypsin and milk

Question 3

what substrate does trypsin act upon?

Answer 3

casein, protein found in milk

Question 4

whats the independent variable

Answer 4

temperature

Question 5

dependant variables?

Answer 5

time taken for milke to become colourless, rate of reaction

Question 6

control variables?

Answer 6

pH, enzyme conc, substrate conc, volume of reactants

Question 7

main purpose of the water bath

Answer 7

maintain a constant temp for reaction

Question 8

how to calculate rate of reaction?

Answer 8

1/time taken

Question 9

why do we repeat?

Answer 9

to ensure reliability and account for anomalies

Question 10

safety precautions?

Answer 10

gloves, goggles ti protect from trypsin, handle glassware accordingly

Question 11

what is the expected trend in enzyme activity as temperature increases?

Answer 11

rate of reaction increases with temp, but beyond optimum enzyme may denature, so enzyme no longer works

Question 12

how can you improve accuracy of timing

Answer 12

use colorimeter to measure change in absorbency as milk becomes colourless