RBC COUNTING (LAB) Flashcards by Aleah Nicole Rosales

The number of red cell in 1 cu. mm

Red cell count

How well did you know this?

Not at all

Perfectly

The red cell count is the number of red cells in ____.

1 cubic millimeter

How well did you know this?

Not at all

Perfectly

The rbc count is one of the test that are used for the diagnosis of ___ and ____.

Anemia
Polycythemia

How well did you know this?

Not at all

Perfectly

Parts of RBC pipette

Calibrated stem (1 unit)
Bulb
Bead (red)
Short stem

How well did you know this?

Not at all

Perfectly

Materials and equipment used in RBC counting

anticoagulated blood
RBC pipette
Diluting fluid
Tally counter
Counting chamber
Gauze pad
Microscope
Test tube

How well did you know this?

Not at all

Perfectly

The RBC pipette should be filled with blood up to?

0.5 mark

How well did you know this?

Not at all

Perfectly

Used to wipe the outside walls of the pipette

Gauze pad to avoid contamination

How well did you know this?

Not at all

Perfectly

Gently rotate the pipette to mix the ____ and ____.

Diluting fluid and blood

How well did you know this?

Not at all

Perfectly

Mix for how many minutes?

5 minutes

How well did you know this?

Not at all

Perfectly

How many drops of the diluted sample must be discarded

3-4 drops

How well did you know this?

Not at all

Perfectly

Position of the hemocytometer in the microscope stage

Horizontal position

How well did you know this?

Not at all

Perfectly

1 large square is made up of how many squares?

9 (1mm x 1mm) squares

How well did you know this?

Not at all

Perfectly

Each WBC squares is divided further into how many squares?

16 squares

How well did you know this?

Not at all

Perfectly

The center square for RBC is subdivded into

25 smaller squares

How well did you know this?

Not at all

Perfectly

Distance between each counting chamber and coverslip (depth)

0.10 millimeter

How well did you know this?

Not at all

Perfectly

Total volume is?

9 cubic millimeters

How well did you know this?

Not at all

Perfectly

Calculate the number of RBC per___

Per liter of each side of the hemocytometer

How well did you know this?

Not at all

Perfectly

Total area counted for RBC counting

1/5 square millimeter (mm²)

How well did you know this?

Not at all

Perfectly

How many parts of blood and how many parts of diluting fluid is needed for the dilution?

1 part of blood to 200 parts diluting fluid
1:200

How well did you know this?

Not at all

Perfectly

To calculate the rbc count per cubic milliliter of blood, simply multiply the _____

Number of rbc counted x area correction factor x depth correction factor x dilution correction factor

How well did you know this?

Not at all

Perfectly

The answer is expressed in

Millions cells per cubic milliliters of blood sample

How well did you know this?

Not at all

Perfectly

We can convert it in SI unit which is ___

Number of cells x 0.000001 =
_____ x 10¹²/L of blood

How well did you know this?

Not at all

Perfectly

Depth counting chamber

0.1 mm

How well did you know this?

Not at all

Perfectly

Depth of counting chamber in fraction

1/10 mm

How well did you know this?

Not at all

Perfectly

Depth **correction factor**

How many parts of blood

1 part of blood

How many parts of diluting fluid

200 parts

Dilution factor for rbc ct

200

Area correction factor for RBC counting

Length of one small square

1/5 mm or 0.2 mm

Width of one small square

1/5 mm or 0.2 mm

Area of one small square

1/5 mm x 1/5 mm = 1/25 mm² or 0.4 mm²

In Rbc counting, we count in how many small squares?

5 small squares

Total area of 5 small squares

1/25mm² X 5 = **1/5 mm²**

Variation in technique

- polycythemia or erythremia - anemia

If px has polycythemia, blood is drawn to what mark? the diluent is up to what mark?

0.3 mark of the rbc pipette and diluent up to 101 mark

Final dilution if the px has polycythemia

1:333

If the px has anemia, up to what mark of the rbc pipette and diluent is aspirated?

1 mark and the diluent to 101

Final dilution if the px has anemia

1:100

Reference values

Normal values: Female: 4.0 - 5.4x 10¹²/L Male: 4.6 - 6.0 x 10¹²/L

Physiologic Variation

1. Increased count in dehydration 2. Increased count in exercise 3. Newborn children have higher counts than adults 4. Women have lower counts than male 5. Individual living at higher altitudes have higher counts

Dehydration results in

Decreased plasma in blood

Pathologic Variation

1. Increased erythrocyte count in polycythemia 2. Increased in pulmonary tuberculosis and pulmonary fibrosis 3. Increase in acute poisoning 4. Decrease in anemia and after hemorrhages

Excessive amount of rbc makes the blood

Thicker than normal

Rules in counting

1. Observe the boundary line of the square 2. Never count cells drying preparation 3. Never count cells underfilled or overfilled chamber

2 boundary lines of the square

A. Double line - **outer line** is the boundary of the square B. Triple Line - **middle line** is the boundary of the square

Counting rule • Do not count cells touching the - - This is to **avoid double counting**

Bottom line Right line

Composition of hayem's fluid

0.5 g of mercuric chloride (antiseptic) 1 g of Sodium chloride (provides isotonicity) 5g of sodium sulphate (prevents rouleaux formation) 200 ml of distilled water (solvent)

Locate the small square using what objective lens?

Low power objective

To visualize the fine lines, adjust the ____, and switch to 10x then 40x

Light intensity

In what specific smaller squares we will count?

1st, 5th, 25th, 21st, 13th

Rule that we use in counting

L rule

5 smaller squares is equal to how many smallest squares?

80 smallest squares

1 smaller square is equal to how many smallest squares?

16 smallest squares

Conditions under Low rbc count/ erythropenia

Anemia Vitamin B deficiency Internal bleeding Kidney diseases Malnutrition

Conditons under High rbc count/ polycythemia

Smoking Congenital heart disease Dehydration Hypoxia Lung disease

Platelet count diluting solution

1% ammonium oxalate 1g ammonium oxalate in 100ml of distilled water

If platelet count is extremely low, use the ___ pipette then follow the formula for ___ count using 1% ammonium oxalate as diluting fluid.

Wbc

Normal platelet count ranges from

140,000 - 450,000 cells/cu.mm

Conditions associated in Low platelet count/ thrombocytopenia

Leukemia and certain other cancers Certain Types of anemia Certain Viral infection Certain Respiratory disorders Radio and chemotherapy Sepsis Certain autoimmune disorders Certain medications and drugs Exposure to toxic substances Alcoholism Cirrhosis Iron and folate deficiency Vitamin B12 deficiency

Conditions associated with High platelet count/thrombocytosis

Bleeding Cancer Certain infections Iron deficiency Removal of spleen Certain types of anemia Certain inflammatory diseases Surgery

Rbc pipette has ___ bulb than Wbc pipette

Larger

Wbc pipette has ___ lumen than rbc pipette

Larger

thick slide with h-shaped groove

Neubauer slide

composition of cover slip and neubauer slide

Neubauer chamber

collection of materials that we need to use

Hemocytometer

used in counting rbc and wbc

Thoma pipette

Diluting fluid for rbc

Hayem's fluid 0.85% normal saline solution Toisson's Fluid

Purpose of RBC Diluting fluid

use to preserve the integrity of the rbc

In wbc, diluting fluid is used to ___

lyse the rbc for better reading of wbcs

Function of Tally counter

Used for rbc counting and sperm analysis

Discarding 3 to 4 drops of the diluted sample just to make sure that the sample charged in the chamber is a ___.

mixed solution (rbc and diluting fluid)

one of the criteria for a quality rbc counting is

Making sure that the chamber is not overfilled or underfilled

We have half way in and half way out and it is only applicable in the

upper line and left line

RBC COUNTING (LAB) Flashcards

(74 cards)