Reagent Strip Parameter Flashcards

1
Q

Specific gravity reagent

A

Bromthymol blue

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2
Q

Specific gravity principle

A

Pka change of poly electrolytes

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3
Q

pH reagent

A

Methyl red and bromthymol blue

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4
Q

pH principle

A

Double indicator

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5
Q

Protein reagent

A

Tetrabromthymol blue

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6
Q

Protein principle

A

Protein error indicator

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7
Q

Glucose reagent

A

Glucose oxidase and peroxidase

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8
Q

Glucose principle

A

Double sequential enzyme

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9
Q

Blood reagent

A

Tetramethyl benzidine

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10
Q

Blood principle

A

Pseudoperoxidase activity of hemoglobin

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11
Q

Ketones reagent

A

Sodium nitroprusside

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12
Q

Ketones principle

A

Sodium nitroprusside reaction

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13
Q

Bilirubin reagent

A

Dichloroaniline diazonium salt

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14
Q

Bilirubin principle

A

Diazo reaction

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15
Q

Urobilinogen reagent

A

P-dimethylaminobenzaldehyde

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16
Q

Urobilinogen principle

A

Ehrlich’s reaction

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17
Q

Nitrite principle

A

Greiss reaction

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18
Q

Nitrite correspond

A

Nitrite correspond 100,000 CFU/mL

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19
Q

Leukocyte reagent

A

Leukocyte esterase

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20
Q

Leukocyte principle

A

Diazo reaction

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21
Q

Label in urine container

A

Name
Age/gender
Record time received

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22
Q

ml of urine needed

A

10-15ml

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23
Q

Errors of urine sample

A

Urine is insufficient
Sample insufficient

24
Q

Determine transparency

A

Clear
Hazy
Cloudy
Turbid
Milky

25
Parameters of reagent strip
Specific gravity pH Protein Glucose Blood Ketones Bilirubin Urobilinogen Nitrite Leukocyte
26
Preliminary information physical examination
Glomerular bleeding Inborn error of metabolites Urinary tract infection Liver disease
27
Time for glucose
30 sec
28
Time for bilirubin
30 sec
29
Time for ketones
40 sec
30
Time for SG
45 sec
31
Time for pH
60 sec
32
Time for protein
60 sec
33
Time for blood
60 sec
34
Time for urobilinogen
60 sec
35
Time for nitrite
60 sec
36
Time for leukocytes
120 sec
37
Presence of casts, increase amount of ________,__________ or ____________indicate urinary tract infection and/or a renal disease.
Pus cell, blood cell or bacteria
38
Presence of casts, increase amount of pus cells, blood cells or bacteria indicate ___________ and/or a _______________.
urinary tract infection and/or a renal disease
39
Centrifuge urine sample of 10-15ml in a tube for about
5 mins
40
Centrifuge urine sample of 10-15ml in a tube for about 5mins of ________ RCF
400 RCF (relative centrifugal force)
41
are identified and counted under a high power objective in at least 10 high power fields. Report as cell/hpf.
Red blood cells, pus cells
42
Examine under low power objective in at least 10 low power fields sediments with low refractive index like
Mucus thread Urates Bacteria Epithelial cells Crystal Cast
43
Report low power
Many Few Moderate
44
Report high power
Cell/hpf
45
presence of large amount of crystals, bacteria, yeast or any microorganism Perform in
Confirmatory testing
46
Bilirubin : neg (b1) Urobilinogen: +++
Hemolytic
47
Bilirubin: - or + (b1 & b2) Urobilinogen: ++
Hepatic
48
Bilirubin: +++ (b2) Urobilinogen: -/dec N
Obstructive
49
Glucose : renal threshold
160-180 mg/dl
50
Presence of intact rbc
Hematuria
51
Intravascular hemolysis
Hemoglobinuria
52
Rhabdomyolysis (increase muscle destruction)
Myoglobinuria
53
Cloudy red or smoky red
Hematuria
54
Clear red
Hemoglobinuria and myoglobinuria
55
Urobilinogen specimen urine
Afternoon urine (2-4 pm)