Reagents Used In Protein Chemistry

Question 1

Cyanogen bromide

Answer 1

It is used for breaking peptide bonds specifically on the carboxyl side that cleaves at methionine residue generating smaller peptide fragment

Question 2

Urea

Answer 2

It is used for denaturation, solubilization and purification

Question 3

Beta mercaptoethanol

Answer 3

It is used for the reduction of disulphide bond in protein

Question 4

Trypsin

Answer 4

It is used in the enzymatic digestion of protein which cleaves peptide bonds on the carboxyl side of amino acids lysine and arginine

Question 5

Performic acid

Answer 5

It enhances the solubility and ionization of proteins,peptides and other biomolecules during chromatographic separation

Question 6

Chymotrypsin

Answer 6

It is used in the enzymatic digestion of protein which target peptide bonds adjacent aromatic amino acids

Question 7

Dabsyl chloride

Answer 7

It is used for labeling and analyzing proteins enabling their chromatographic separation, detection and identification

Question 8

Fluorodinitrobenzene

Answer 8

It is used to selectively modify and label the amino group of the N terminus or the side chain of lysine residue

Question 9

6N HCl

Answer 9

It is used for the hydrolysis of protein

Question 10

Ninhydrin

Answer 10

It is used to detect and visualize amino acids and peptides. It forma colored compounds

Question 11

Hydroxylamine

Answer 11

It is used to cleave asparagine and glycine peptide bonds respectively