Regulation of cellular pH Flashcards Preview

BMS 242 - Core Phys/pharm > Regulation of cellular pH > Flashcards

Flashcards in Regulation of cellular pH Deck (39)
Loading flashcards...
1

What is the pH of the plasma?

7.4

2

What is the pH inside the cell?

7-7.1

3

What scale is pH? (equation)

What does this mean?

Logarithmic scale:
pH = -log[H+]

1 small change in H+ has a LARGE change in pH

4

How do proteins buffer pH?

1) H+ binds to protein - changes the protein charge
2) Conformational change in the protein
3) Change in protein function

5

Why can increase in H+ concentration potentially have disastrous effects?

H+ binds to proteins and changes their charge and therefore shape and FUNCTION

6

What 2 methods can be used to measure intracellular pH?

1) Microelectrodes

2) Fluorescent indicators

7

Which method is good for measuring pH of large cells?

Describe this method

Microelectrodes:

1) 2 voltage electrodes - measure ion current across the membrane

2) Measure voltage difference between V1 and V2

3) Change in voltage is proportional to pH

8

How are the electrodes in the microelectrode technique calibrated?

1) Buffer at pH 6 and measure voltage

2) Buffer at pH 8 and measure voltage

3) Construct a curve

9

How can voltage be converted into pH?

Using the curve constructed:
pH = (V-offset) / slope

10

Which method is good for measuring pH of small cells?

Describe this method

Fluorescent indicators:

1) Cells loaded with LIPID SOLUBLE, INACTIVE form of the indicator

2) Inside the cell - the inactive form is converted into an active form

3) Indicator is excited with light of a specific wavelength and the amount of EMITTED fluorescence light is measured (emits at a different wavelength)

4) Fluorescence is proportional to intracellular pH and [H+]

11

In the fluorescent indicator method to measure pH, why is the cell loaded with an INACTIVE form of the indicator?

It is positively charged - so it can cross the membrane

12

In the fluorescent indicator method to measure pH, how is the inactive form of the indicator turned into the active form?

What does this cause?

Inactive form is cleaved

Active form has a negative charge - is TRAPPED inside the cell

13

How is the fluorescent indicator method calibrated?

At the end of the experiment:

- Proton ionophore which calibrates pH inside of the cell with the pH outside

- When change pH outside of the cell, pH inside the cell becomes the same

- Look at the signal intensity when the pH changes

14

What 3 things are involved in the control of intracellular pH?

1) Buffering
2) Acid extrusion
3) Acid loading

15

What is the relationship between acid extrusion and acid loading in a cell under normal conditions?

What do these systems do?

They are balanced

These systems act to REVERSE pH changes

16

What is 'buffering power'?

The amount of strong base that must be added to a solution in order to raise the pH by a given amount

17

What buffers inside the cell?

How?

Proteins

Reversibly consume or release protons
Using their COOH and NH2 groups

18

What can buffers do and what can't they do?

Can MINIMISE pH changes

Cant REVERSE or PREVENT pH changes

19

What happens if pH increases (in regards to buffers)?

COOH in the protein buffer releases a H+
COO-

20

What happens if pH decreases (in regards to buffers)?

NH2 in the protein buffer accepts a H+
NH3+

21

Which transport protein performs acid extrusion and what does this channel rely on?

Na/H exchanger (Na in, H out)

Relies upon the inward Na gradient created by Na/K ATPase

22

What is allosteric modification?

- H+ which aren't being transported out of the exchanger BIND to the exchanger

- Leading to a conformational change of the exchanger and amplification of activity

- Increased acid extrusion

23

At normal pH conditions, what does the Na/H exchanger work on?

The Na gradient

24

What is the structure of the Na/H exchanger?

- 12 transmembrane domains

- Between transmembrane 4 and 5 is the Na+ and H+ binding and exchange region

- Large intracellular C domain which is the regulating region

25

What is the name for the Na/H exchanger?

NHE1

26

What function does NHE1 have?

Housekeeping function:

- Regulates pH inside the cell and cell volume

27

What is NHE1 (Na/H exchanger) inhibited by?

Amiloride and EIPA (analogue of amiloride)

28

Which transport protein is involved in acid loading?

How does this work?

Cl/HCO3 exchanger:

- HCO3- out and Cl into the cell
- Removal of HCO3- leaves H+ behind, causing acidification

29

What is the name of the Cl/HCO3 exchanger?

AE (anion exchanger)

30

What is an example of an anion exchanger?

Band 3